This is description of this part:
Leica SFL100 530 nm - LED fluorescence light source for Leica DMi8 / DM1000-DM3000 microscopes
Basic parameters:
Manufacturer: Leica
Model: Leica SFL100 530 nm
Manufacturer part number: 11504195
Light source type: LED
Wavelength: 530 nm
Light colour: green (TRITC)
Microscope mounting method: dovetail mount
Mounting diameter: 48 mm
Compatible microscopes: Leica DMi8 / DM1000 / DM2000 / DM2500 / DM3000
Compatible filter cube: 530 nm LED (for example P/N 11504156)
ON/OFF switch: integrated into the illuminator together with a potentiometer for light intensity adjustment
This illuminator provides intense green fluorescence illumination for:
- Leica DMi8 inverted microscope
- Leica DM1000-DM3000 routine microscopes
Similar illuminators with different light colours (wavelengths) include:
- P/N 11504196 Leica SFL100, 365 nm
- P/N 11504138 Leica SFL100, 470 nm
Important:
- the included power supply is not an original Leica power supply - it is a compatible unit manufactured by another company
- to work effectively with this wavelength, the microscope must be equipped with a suitable optical filter set (a fluorescence filter cube)
- due to legal safety regulations, this LED illuminator is not available within the U.S.A.
- when working with fluorescence illumination, care should always be taken to avoid eye damage
Light at 530 nm is commonly used in two optical configurations.
1. As excitation light (Excitation). It excites fluorescent dyes with yellow, orange and red emission spectra:
- Cy3 / Alexa Fluor 546 / Alexa Fluor 555 - widely used markers in immunofluorescence
- TRITC / Rhodamine - classic rhodamine dyes used for antibody and cytoskeleton (actin) labelling
- dTomato / mStrawberry - red fluorescent proteins used in in vivo studies
2. As an emission detection band (Emission). In the classic blue-light excitation configuration (for example with a 488 nm laser), 530 nm corresponds to the emission maximum (green fluorescence) of:
- GFP (Green Fluorescent Protein) - a standard marker in genetic engineering and cell biology
- FITC / Alexa Fluor 488 - the most commonly used dyes for labelling cellular structures
- Fluo-4 / Calcein - indicators used to measure calcium ion concentration and cell viability
This illuminator does not use any unusual microscope mounting method - it employs a standard 48 mm dovetail mount. As a result, it may be possible to adapt it to other Leitz or Leica microscopes and even to microscopes manufactured by other companies.
What is this part needed for?
- if you own a compatible fluorescence microscope but do not have a light source
- if you need a 530 nm green fluorescence illuminator for your microscope
- if you would like to install this illuminator on a microscope model other than those listed in the description or on a microscope from another manufacturer with a similar illuminator mounting diameter
- if you would like to use this LED light source in your own DIY projects
