This is description of this part:
Complete 4FL fluorescence reflector for Carl Zeiss fluorescence microscopes. This microscope element carries Carl Zeiss part number 446425 (alternative notation: 44 64 25).
This is a four-position fluorescence reflector / fluorescence slider / filter cube carrier used in fluorescence microscopy. The element contains four positions for fluorescence filter cubes or fluorescence filter sets.
The offered element contains the following fluorescence filter cubes:
I.
- Excitation: G365
- Beam splitter: FT395
- Barrier filter: LP420
This filter set is typically used for UV fluorescence applications including:
- DAPI
- Hoechst
- AMCA
- DyLight 350
- other UV excited fluorophores
II.
- Excitation: BP450-490
- Beam splitter: FT510
- Barrier filter: LP515
This filter set is typically used for:
- FITC
- GFP
- Fluorescein
- Alexa Fluor 488
- green fluorescence dyes
III.
- empty slot without filter cube
The free slot allows:
- installation of an additional filter set
- operation without fluorescence filters
- faster switching between fluorescence techniques
IV.
- Excitation: BP546
- Beam splitter: FT580
- Barrier filter: LP590
This filter set is typically used for:
- Rhodamine
- TRITC
- Cy3
- Texas Red
- red fluorescence dyes
Such fluorescence filters are commonly used in:
- cell biology
- histology
- cytology
- immunology
- microbiology
- laboratory diagnostics
- molecular biology
- DNA and RNA analysis
- fluorochrome analysis
- observation of fluorescently labelled antibodies
- tissue autofluorescence studies
- observation of chlorophyll and plant specimens
- analysis of FITC, DAPI, AMCA, Rhodamine and related fluorescent dyes
- fluorescence photomicrography
- material science and technical analysis
- observation of UV fluorescent minerals and substances
Such filter sets are also popular among hobbyists and fluorescence microscopy enthusiasts:
- observing insect autofluorescence
- observing UV fluorescent minerals
- fluorescence photography
- observing fungi, pollen and biological structures
- experiments with fluorescent dyes
This fluorescence reflector is compatible among others with the following Carl Zeiss microscopes:
- Carl Zeiss Axioplan
- Carl Zeiss Axioskop
- Carl Zeiss Axiophot
- Carl Zeiss Axiovert
- other Carl Zeiss fluorescence microscopes using the 4FL system
Different names are commonly used for this element, including:
- fluorescence reflector
- fluorescence slider
- fluorescence module
- filter cube carrier
- filter cube holder
- fluorescence turret
- fluorescence filter holder
- fluorescence filter cube
- fluorescence cassette
- fluorescence insert
- fluorescence block
What is this part needed for?
- when additional fluorescence channels are required
- when a damaged fluorescence reflector must be replaced
- when a microscope is being upgraded for fluorescence microscopy
- when fast switching between UV, green and red fluorescence is required
- when an additional free slot for another filter set is needed
- when a used microscope from the secondary market is incomplete
- when existing filter cubes are damaged or scratched
