Home page of the microscope service parts, repair and spare parts online catalog - Sermik-Oplatek Mikroskopservice Dresden f.n.n.Z

Description of this part for the microscope.

This filter is mounted in the sheet-metal housing of the large HBO 250W fluorescence illuminator. This illuminator is part of a large mirror light beam splitter used in Carl Zeiss Jena Jenalumar microscopes. The KG filter is designed to absorb heat (infrared radiation) emitted by the high-pressure HBO mercury lamp.

Please note that there is also a version of the Carl Zeiss Jena Jenalumar microscopes with a small 50W HBO illuminator. This KG filter glass was removed from a large 250W illuminator.

On the used market, in microscopy forums and in microscopy groups, several names are used for this filter, all referring to the same type of filter:
- KG filter / KG glass
- heat filter
- heat absorbing filter
- heat absorption filter
- heat-absorbing glass
- heat absorption glass
- absorption filter
- IR filter
- infrared blocking filter
- infrared cut filter
- KG3 heat absorption filter

This microscope element is highly susceptible to damage. When this happens, the filter needs to be replaced, for example with the filter presented here. Here are examples of damage that can occur during use of the fluorescence illuminator and reasons why replacement of the filter may be necessary:
- the KG filter may crack or become cloudy due to overheating
- the filter may also chip or break as a result of mechanical damage, for example during transport
- the filter may sometimes be damaged during improper maintenance

High-performance heat filters such as this KG filter used with a 250W lamp are very often used by people building their own DIY projects. The housing with screw mounting makes this particularly convenient.

The last photo in the gallery shows the housing of the HBO 250W illuminator (lampenhaus), with an arrow indicating which element is being described and where it is located inside the illuminator. This makes it possible to identify the element unambiguously.

Description of this part for the microscope.

reflector, performs two functions:
- it contains a barrier filter (emission filter) for the 450 nm wavelength
- it acts as an angular mirror, reflecting the light beam from the EPI fluorescence illuminator vertically downward through the objective toward the specimen.

This fluorescence filter cube fits the following EPI fluorescence microscopes:
- Carl Zeiss Jena Fluoval Fluoreszenz
- Carl Zeiss Jena Fluoval-2 (built on the Carl Zeiss Jena Amplival microscope platform)

Important – this microscope component does not fit the first version of the Carl Zeiss Jena Fluoval microscope. The Fluoval microscope (without "2") does not use a filter cube. Instead, it has a simple permanently mounted swing-out barrier filter.

The filter cube has two positions:
- empty, for normal transmitted-light operation
- 450 nm for reflected-light fluorescence (EPI) operation

Mechanically, this filter cube is installed between the objective nosepiece and the microscope head. It slides into a dedicated slot and moves along a dovetail guide. On the Carl Zeiss Jena Laboval Fluoreszenz microscope, this slot is located in the additional upper horizontal arm of the EPI fluorescence illuminator. On the Carl Zeiss Jena Fluoval-2 microscope, the slot is located in the EPI fluorescence arm, which is an integral part of the microscope. The Fluoval-2 is based on the Carl Zeiss Jena Amplival microscope, which has a modular design. Mechanically, the Fluoval-2 is essentially an Amplival equipped with an upper EPI fluorescence arm.

Sliding the cube along this dovetail guide switches between the empty position (transmitted light) and EPI fluorescence with the 450 nm filter.

Description of this part for the microscope.

inside the microscope base. It includes a mechanism that allows these filters to be inserted into or removed from the light path using control buttons. Since more than one filter can be placed in the light path at the same time, the mechanism also includes an additional (seventh) button that removes all filters from the light path simultaneously. Because of this seventh button, some descriptions incorrectly state that the microscope base contains seven filters.

The following filters are installed in this filter cassette:
- NCB filter (blue conversion filter that transforms yellow incandescent lamp light into naturally white light), Zeiss P/N 46 78 50 (yellow stripe on the push button)
- VG 9 green filter, Zeiss P/N 46 78 05 (green stripe on the push button)
- two neutral density filters 0.5, Zeiss P/N 46 78 40 (light grey stripes on the push button)
- neutral density filter 0.12, Zeiss P/N 46 78 41 (grey stripe on the push button)
- neutral density filter 0.03, Zeiss P/N 46 78 42 (dark grey stripe on the push button)

Combinations of the neutral density filters provide stepwise light transmission values ranging from 1.5% to 100%.

Each of these filters can be removed from its holder, resulting in a standard 32 mm round microscope filter that can also be used in microscopes made by other manufacturers. In addition, any other 32 mm round filter can be installed in this filter cassette, allowing the filter set of the microscope to be configured freely.

This filter cassette with its complete control mechanism fits all versions of Carl Zeiss Photomicroscope microscopes (also known as Zeiss PM or Zeiss Phomi) as well as Zeiss Universal microscopes. However, there are also some less common variants of the Zeiss Universal that do not include this filter mechanism inside the microscope base. Importantly, the Carl Zeiss Ultraphot microscope does not use this component.

The last photographs in the gallery indicate with an arrow exactly which component is being offered and where it is located inside the microscope. This allows unambiguous identification of the part.

Description of this part for the microscope.

parameters:
Filter colour: blue, matt
Function: NCB – converts the yellow light of the halogen lamp into naturally white light
Mounting diameter: adjustable from 25.5 mm to 26 mm

Mechanically, this filter is mounted on the front end of the halogen illuminator (over the light output opening). Its effect is to produce light with a colour similar to that emitted by a cool white LED.

Description of this part for the microscope.

light from the lamp into homogeneous illumination, makes the lamp filament invisible and ensures that the entire specimen is illuminated evenly.

The filter is mounted on a swing-out holder, which is a separate module installed below the condenser. This allows the entire frosted diffuser to be swung out of the optical path. In this position, for example, illumination according to the Koehler standard can be adjusted.

Users employ various names for the matt filter mounted below the condenser. These include:
- matt filter
- frosted filter
- ground glass diffuser
- diffuser
- diffuser filter
- frost filter
- opal diffuser
- diffusing screen
- condenser diffuser
- swing-out diffuser

In the last photograph, an arrow indicates which microscope element is being offered and where it is located in the microscope, allowing this part to be identified unambiguously.

The holder with the frosted diffuser fits every model of the Lomo Polam microscope series (the model designation written in Cyrillic is given in parentheses):
- Lomo Polam S-111 (the Cyrillic designation looks like C-111)
- Lomo Polam S-112 (C-112)
- Lomo Polam R-111 (P-111)
- Lomo Polam R-112 (P-112)
- Lomo Polam R-113 (P-113)
- Lomo Polam L-211
- Lomo Polam L-213
- Lomo Polam LMPR
- also Lomo Polam R-312 and Lomo Polam R-311 (the Cyrillic designation is P-312 and P-311), although in these models the matt filter below the condenser is usually not required because these microscopes are intended primarily for reflected light work

The final photograph, in which I indicate with an arrow the mounting position of the diffuser below the condenser, is a poster containing a comparison table of various Lomo Polam microscope models. It will help you easily identify which model of Lomo Polam polarizing microscope you own.

Description of this part for the microscope.

diffuser)
- blue
- yellow
- green

New (usually Chinese) microscope filters are sometimes not even made of glass. And even if they are made of glass - you may encounter scandalous examples where the glass is only surface-colored. Unfortunately, cheap colored glass filters may not be internally homogeneous and can cause light refraction distortions.

This filter set by PZO was originally sold together with the PZO Biolar microscope. It was manufactured in the 1970s (and certainly before 1977), so these are optical glasses that are about half a century old! They have never been used until now (this is so-called NOS - New Old Stock), therefore these are excellent old-class optical glasses of the highest quality.

Filters with a 32 mm diameter are used - and can be used - in microscopes from various manufacturers:
- Carl Zeiss Jena
- Zeiss West Germany
- Eschenbach
- Meopta
- Nikon
- ROW
- Leitz Wetzlar
- Wild
- Hund
- Kosmos
- and many others

Important - popular microscopes by Lomo (for example Lomo Biolam) have a 33 mm filter holder, so the filters shown here have too small a diameter and may fall out from some Lomo holders.

Description of this part for the microscope.

25).

This is a four-position fluorescence reflector / fluorescence slider / filter cube carrier used in fluorescence microscopy. The element contains four positions for fluorescence filter cubes or fluorescence filter sets.

The offered element contains the following fluorescence filter cubes:

I.
- Excitation: G365
- Beam splitter: FT395
- Barrier filter: LP420

This filter set is typically used for UV fluorescence applications including:
- DAPI
- Hoechst
- AMCA
- DyLight 350
- other UV excited fluorophores

II.
- Excitation: BP450-490
- Beam splitter: FT510
- Barrier filter: LP515

This filter set is typically used for:
- FITC
- GFP
- Fluorescein
- Alexa Fluor 488
- green fluorescence dyes

III.
- empty slot without filter cube

The free slot allows:
- installation of an additional filter set
- operation without fluorescence filters
- faster switching between fluorescence techniques

IV.
- Excitation: BP546
- Beam splitter: FT580
- Barrier filter: LP590

This filter set is typically used for:
- Rhodamine
- TRITC
- Cy3
- Texas Red
- red fluorescence dyes

Such fluorescence filters are commonly used in:
- cell biology
- histology
- cytology
- immunology
- microbiology
- laboratory diagnostics
- molecular biology
- DNA and RNA analysis
- fluorochrome analysis
- observation of fluorescently labelled antibodies
- tissue autofluorescence studies
- observation of chlorophyll and plant specimens
- analysis of FITC, DAPI, AMCA, Rhodamine and related fluorescent dyes
- fluorescence photomicrography
- material science and technical analysis
- observation of UV fluorescent minerals and substances

Such filter sets are also popular among hobbyists and fluorescence microscopy enthusiasts:
- observing insect autofluorescence
- observing UV fluorescent minerals
- fluorescence photography
- observing fungi, pollen and biological structures
- experiments with fluorescent dyes

This fluorescence reflector is compatible among others with the following Carl Zeiss microscopes:
- Carl Zeiss Axioplan
- Carl Zeiss Axioskop
- Carl Zeiss Axiophot
- Carl Zeiss Axiovert
- other Carl Zeiss fluorescence microscopes using the 4FL system

Different names are commonly used for this element, including:
- fluorescence reflector
- fluorescence slider
- fluorescence module
- filter cube carrier
- filter cube holder
- fluorescence turret
- fluorescence filter holder
- fluorescence filter cube
- fluorescence cassette
- fluorescence insert
- fluorescence block

Description of this part for the microscope.

metal plate which presses these filters against the microscope housing. It consists of a thin frosted filter and a thick filter which, in my opinion, performs light color correction and also absorbs heat from the halogen bulb.

This is a part removed from the stand of an Olympus BX40 microscope. It has been tested and cleaned - and is suitable for use as a service element for repairing a damaged microscope. Many smaller Olympus service elements do not have their own catalog numbers or commercial names, therefore in the last photos in the gallery I indicate with an arrow exactly which element is being offered and where it is located inside the microscope. In this description some names used are descriptive rather than official technical commercial names.

Olympus BX series microscopes use identical assemblies in many different models, therefore there is a high probability that this element - although removed from a BX40 model - is also compatible with other BX series models (for example BX41, BX43, BX50, BX51 etc.). However, before deciding to install such an element in an Olympus BX microscope, it is advisable to first carefully compare shapes and dimensions or ask appropriate questions on forums and in Olympus BX microscope user groups.

Description of this part for the microscope.

catalog number 451495-0100 (45 14 59 - 0100).

The basic versions of the Zeiss Axioskop 20 microscope, intended only for transmitted light operation, do not include any filter cube. In such cases, this type of blanking cover is required to prevent dust from entering the optics.

Description of this part for the microscope.

filter holder of Carl Zeiss Jena Vertival microscopes.

Description of this part for the microscope.

rare on the used microscope market. It can be used with microscopes such as:
- Carl Zeiss Jena Jenamed Fluorescence (Fluoreszenz)
- Carl Zeiss Jena Jenalumar
- Carl Zeiss Jena Fluoval
- Carl Zeiss Jena Laboval Fluorescence

Description of this part for the microscope.

rare on the used microscope market. It can be used with microscopes such as:
- Carl Zeiss Jena Jenamed Fluorescence (Fluoreszenz)
- Carl Zeiss Jena Jenalumar
- Carl Zeiss Jena Fluoval
- Carl Zeiss Jena Laboval Fluorescence

Description of this part for the microscope.

rare on the used microscope market. It can be used with microscopes such as:
- Carl Zeiss Jena Jenamed Fluorescence (Fluoreszenz)
- Carl Zeiss Jena Jenalumar
- Carl Zeiss Jena Fluoval
- Carl Zeiss Jena Laboval Fluorescence

Description of this part for the microscope.

be used with any microscope from any manufacturer. It is most often used as a polarizer.

Note - in commonly used terminology there is some inconsistency and foreign as well as incorrect names are often used:
- polaryzator
- polarisator
- polarizer
- polariser
- filtr polaryzacyjny
- filtr POL
- circular polariser
- CPL Filter
- POL filter
- polarisations Filter
- polarization Filter
- anaizator
- analisaor
- Analysator
- POL analizer
- polarization analyser

This part may be the answer to the following problems:
- How do I achieve polarization in a microscope?
- I'm looking for a polarizer for my Lomo microscope
- My Lomo polarizer has delamination
- I have a discolored polarizer in my microscope
- Where can I buy a polarizing filter for my microscope?

Description of this part for the microscope.

type: GREEN channel

Typically used for fluorophores such as:
- FITC
- GFP
- Alexa Fluor 488

excitation filter: approx. 470-495 nm
dichroic mirror: approx. 505 nm
emission filter: approx. 515-550 nm

Applications:
- GFP imaging
- FITC immunofluorescence
- general green fluorescence

Description of this part for the microscope.

DAPI

Typically used for fluorophores such as:
- DAPI
- Hoechst
- other DNA stains

excitation filter: approx. 350 +/- 25 nm
dichroic mirror: approx. 400 nm LP
emission filter: approx. 460 +/- 25 nm

excitation: UV
emission: blue fluorescence from DNA

Applications:
- nuclear staining
- histology
- cytogenetics
- cell microscopy

Description of this part for the microscope.

Orange#1 FISH
FISH = Fluorescence In Situ Hybridization

Typically used for Orange-1 / TRITC fluorophores such as:
- Cy3
- Orange dyes
- Rhodamine
- FISH probes

excitation filter: approx. 540-565 nm
dichroic mirror: 565 nm
emission filter: approx. 590 +/- 33 nm

Applications:
- genetic diagnostics
- chromosome detection
- DNA research

Description of this part for the microscope.

ET
Catalogue number: 11504171
Channel type: Y5ET is a filter designed for fluorophores in the yellow-green excitation range.

Typical fluorophores:
- GFP variants
- YFP
- Alexa Fluor 514
- FITC-like dyes

Applications:
- immunofluorescence
- cell microscopy
- cell culture research

Description of this part for the microscope.

a glass frosted filter (so-called ground glass) is located in the optical light beam path. It is mounted on a steel rod ending with a handle positioned outside the microscope base - at the rear, next to the place where the illuminator is inserted. This handle is used to insert and withdraw the frosted filter from the illumination beam path.

This design often leads to damage:
- the glass frosted filter is mounted on the rod in such a way that the glass frequently cracks
- the handle located at the rear of the microscope base very often breaks or crumbles
- the metal rod often jams and as a result of attempts to move it - bends

Here I present and offer this exact frosted filter with the complete movement mechanism:
- glass frosted filter (ground glass)
- holder for mounting the filter to the rod
- all necessary screws to attach this set to the microscope base
- plastic control knob for operating the ground glass

Description of this part for the microscope.

This element is in perfect condition and shows no signs of use.

Note - this is not a flat slide like most "sliders". It is a cube (in German "Würfel") with a knob for rotating the polarizer and mirrors inside.

Description of this part for the microscope.

mm
Thickness: 2 mm

This filter is originally glued into the filter wheel located in the microscope above the objective turret.

Description of this part for the microscope.

"larger" circle with mirror filters that is built into the head.

Installed filters:
- yellow
- red
- green
- blue
- empty slot

Description of this part for the microscope.

rare on the used microscope market. It can be used with microscopes such as:
- Carl Zeiss Jena Jenamed Fluorescence (Fluoreszenz)
- Carl Zeiss Jena Jenalumar
- Carl Zeiss Jena Fluoval
- Carl Zeiss Jena Laboval Fluorescence

Description of this part for the microscope.

filter is exceptionally rare on the used microscope market. It can be used with microscopes such as:
- Carl Zeiss Jena Jenamed Fluorescence (Fluoreszenz)
- Carl Zeiss Jena Jenalumar
- Carl Zeiss Jena Fluoval
- Carl Zeiss Jena Laboval Fluorescence

Description of this part for the microscope.

and other fluorescence microscopes from this company.

Description of this part for the microscope.

Description of this part for the microscope.

PZO logo and a circular illumination filter - the one offered here is colorless.

Some newer versions of the PZO Biolar microscope included a blue illumination filter mounted in the microscope base. This filter cut out the yellow color of the light emitted by the bulb, providing the user with a pleasant, near-white light.

Unfortunately, this filter is not replaceable, and when the light source is changed to a halogen or cold LED, the blue light filter becomes annoying, as the light color changes from white to blue.

If the user tries to remove the original light filter themselves, they often break either the filter or its black housing. This brings us to the point where it's worth having this part on hand - you can easily swap the blue filter for a colorless one.

NOTE - this part is originally heat-attached and held in place by melted plastic legs. After replacement, it's best to glue it in place.

This item fits the following versions of the Biolar PZO microscope:
- B
- Pol
- DIC
- EPI
- Fluoreszenz

Note - it likely will not fit the B2 or GF versions.