Home page of the microscope service parts, repair and spare parts online catalog - Sermik-Oplatek Mikroskopservice Dresden f.n.n.Z

Description of this part for the microscope.

rare on the used microscope market. It can be used with microscopes such as:
- Carl Zeiss Jena Jenamed Fluorescence (Fluoreszenz)
- Carl Zeiss Jena Jenalumar
- Carl Zeiss Jena Fluoval
- Carl Zeiss Jena Laboval Fluorescence

Description of this part for the microscope.

filter is exceptionally rare on the used microscope market. It can be used with microscopes such as:
- Carl Zeiss Jena Jenamed Fluorescence (Fluoreszenz)
- Carl Zeiss Jena Jenalumar
- Carl Zeiss Jena Fluoval
- Carl Zeiss Jena Laboval Fluorescence

Description of this part for the microscope.

rare on the used microscope market. It can be used with microscopes such as:
- Carl Zeiss Jena Jenamed Fluorescence (Fluoreszenz)
- Carl Zeiss Jena Jenalumar
- Carl Zeiss Jena Fluoval
- Carl Zeiss Jena Laboval Fluorescence

Description of this part for the microscope.

rare on the used microscope market. It can be used with microscopes such as:
- Carl Zeiss Jena Jenamed Fluorescence (Fluoreszenz)
- Carl Zeiss Jena Jenalumar
- Carl Zeiss Jena Fluoval
- Carl Zeiss Jena Laboval Fluorescence

Description of this part for the microscope.

rare on the used microscope market. It can be used with microscopes such as:
- Carl Zeiss Jena Jenamed Fluorescence (Fluoreszenz)
- Carl Zeiss Jena Jenalumar
- Carl Zeiss Jena Fluoval
- Carl Zeiss Jena Laboval Fluorescence

Description of this part for the microscope.

diffuser)
- blue
- yellow
- green

New (usually Chinese) microscope filters are sometimes not even made of glass. And even if they are made of glass - you may encounter scandalous examples where the glass is only surface-colored. Unfortunately, cheap colored glass filters may not be internally homogeneous and can cause light refraction distortions.

This filter set by PZO was originally sold together with the PZO Biolar microscope. It was manufactured in the 1970s (and certainly before 1977), so these are optical glasses that are about half a century old! They have never been used until now (this is so-called NOS - New Old Stock), therefore these are excellent old-class optical glasses of the highest quality.

Filters with a 32 mm diameter are used - and can be used - in microscopes from various manufacturers:
- Carl Zeiss Jena
- Zeiss West Germany
- Eschenbach
- Meopta
- Nikon
- ROW
- Leitz Wetzlar
- Wild
- Hund
- Kosmos
- and many others

Important - popular microscopes by Lomo (for example Lomo Biolam) have a 33 mm filter holder, so the filters shown here have too small a diameter and may fall out from some Lomo holders.

Description of this part for the microscope.

reflector, performs two functions:
- it contains a barrier filter (emission filter) for the 450 nm wavelength
- it acts as an angular mirror, reflecting the light beam from the EPI fluorescence illuminator vertically downward through the objective toward the specimen.

This fluorescence filter cube fits the following EPI fluorescence microscopes:
- Carl Zeiss Jena Fluoval Fluoreszenz
- Carl Zeiss Jena Fluoval-2 (built on the Carl Zeiss Jena Amplival microscope platform)

Important - this microscope component does not fit the first version of the Carl Zeiss Jena Fluoval microscope. The Fluoval microscope (without "2") does not use a filter cube. Instead, it has a simple permanently mounted swing-out barrier filter.

The filter cube has two positions:
- empty, for normal transmitted-light operation
- 450 nm for reflected-light fluorescence (EPI) operation

Mechanically, this filter cube is installed between the objective nosepiece and the microscope head. It slides into a dedicated slot and moves along a dovetail guide. On the Carl Zeiss Jena Laboval Fluoreszenz microscope, this slot is located in the additional upper horizontal arm of the EPI fluorescence illuminator. On the Carl Zeiss Jena Fluoval-2 microscope, the slot is located in the EPI fluorescence arm, which is an integral part of the microscope. The Fluoval-2 is based on the Carl Zeiss Jena Amplival microscope, which has a modular design. Mechanically, the Fluoval-2 is essentially an Amplival equipped with an upper EPI fluorescence arm.

Sliding the cube along this dovetail guide switches between the empty position (transmitted light) and EPI fluorescence with the 450 nm filter.

Description of this part for the microscope.

catalogue number: 46 78 36 (also written as 467836)
Colour: blue matt
Diameter: 32 mm
Material: glass

This is a filter that combines the functions of a blue filter and a ground glass diffuser:
- the blue filter is used to convert the yellow, warm light of the bulb into light closer to white light
- the matt filter (called a diffuser or ground glass) is used to even out the light across the whole area of the specimen and to remove the visible filament of the bulb

This does, however, have one consequence: without a visible filament it is not possible to work with illumination set up according to the Koehler method - and Zeiss mentions this in the leaflet supplied with the filter.

Very often only one or two microscope filters can be fitted in a microscope, for example one on the illuminator and the second in the holder below the condenser. The fact that the glass filter offered here is a 2-in-1 solution makes it possible to save the possible second slot for another filter.

The diameter of 32 mm is a standard with many manufacturers, which means this filter can be used with microscopes of numerous brands. The "compatibility" section lists over a dozen such microscopes, but it is not a complete list.

Description of this part for the microscope.

Carl Zeiss Jena
Model: KP425
Type: interference excitation filter for fluorescence
Diameter: 32 mm
Mirror coating: yes

Filters 32 mm in diameter can be used in most microscopes from many manufacturers. In Carl Zeiss Jena microscopes they can be used, among others, in the following fluorescence microscopes:
- Carl Zeiss Jena Jenamed Fluorescence (Fluoreszenz)
- Carl Zeiss Jena Jenalumar
- Carl Zeiss Jena Fluoval
- Carl Zeiss Jena Laboval Fluorescence

Note - because this is an interference filter (it has a mirrored surface), the angle at which the filter sits relative to the incoming light matters and affects its parameters.

The coating on interference filters is sensitive not only to mechanical damage, for example scratches from improper storage or cleaning. Several decades of work right next to the HBO lamp can cloud it or make it delaminate, which shows as patches or rainbow islands that do not disappear when you rotate it. In that case the best solution is to replace the filter with one like the one I offer here.

Description of this part for the microscope.

Carl Zeiss Jena
Model: KP490
Type: interference excitation filter for fluorescence
Diameter: 32 mm
Mirror coating: yes

Filters 32 mm in diameter can be used in most microscopes from many manufacturers. In Carl Zeiss Jena microscopes they can be used, among others, in the following fluorescence microscopes:
- Carl Zeiss Jena Jenamed Fluorescence (Fluoreszenz)
- Carl Zeiss Jena Jenalumar
- Carl Zeiss Jena Fluoval
- Carl Zeiss Jena Laboval Fluorescence

Note - this is an interference filter, which you can tell from the mirrored surface. For that reason the angle at which the filter sits relative to the incoming light matters and affects its parameters.

The coating on interference filters is sensitive to mechanical damage, for example scratches from improper storage or cleaning. Age is a separate matter - several decades of work right next to the HBO lamp can cloud it or make it delaminate, which shows as patches or rainbow islands that do not disappear when you rotate it. In that case the best solution is to replace the filter with one like the one I offer here.

Description of this part for the microscope.

Carl Zeiss Jena
Model: KP560
Type: interference excitation filter for fluorescence
Diameter: 32 mm
Mirror coating: yes

Filters 32 mm in diameter can be used in most microscopes from many manufacturers. In Carl Zeiss Jena microscopes they can be used, among others, in the following fluorescence microscopes:
- Carl Zeiss Jena Jenamed Fluorescence (Fluoreszenz)
- Carl Zeiss Jena Jenalumar
- Carl Zeiss Jena Fluoval
- Carl Zeiss Jena Laboval Fluorescence

Note - the mirrored surface gives away that this is an interference filter. With filters of this kind the angle to the incoming light is not indifferent and affects their parameters.

The coating on interference filters is sensitive to mechanical damage, for example scratches from improper storage or cleaning. The second enemy is time - several decades of work right next to the HBO lamp can cloud it or make it delaminate, which shows as patches or rainbow islands that do not disappear when you rotate it. In that case the best solution is to replace the filter with one like the one I offer here.

Description of this part for the microscope.

Carl Zeiss Jena
Model: B226g
Filter type: red-absorbing glass filter (the manufacturer calls it Rotsperrfilter)
Diameter: 32 mm
Colour: blue
Toughened version: yes

The letter "g" at the end of the designation is short for the German gehärtet, meaning toughened - the manufacturer gave it to filters meant to work right next to high-power lamps, especially HBO mercury burners, where ordinary glass does not withstand the high temperature.

Filters 32 mm in diameter can be used in most microscopes from many manufacturers. In Carl Zeiss Jena microscopes they can be used, among others, in the following fluorescence microscopes:
- Carl Zeiss Jena Jenamed Fluorescence (Fluoreszenz)
- Carl Zeiss Jena Jenalumar
- Carl Zeiss Jena Fluoval / Fluoval-2
- Carl Zeiss Jena Laboval Fluorescence

In the excitation path this filter does not work on its own. It is added to the main filter to cut off the red passed by that filter and to narrow the excitation band further.

Description of this part for the microscope.

Carl Zeiss Jena
Model: B228g
Filter type: red-absorbing glass filter (the manufacturer calls it Rotsperrfilter)
Diameter: 32 mm
Colour: blue
Toughened version: yes

The "g" at the end of the designation stands for the German gehärtet, meaning toughened. The manufacturer gave it to filters meant to work right next to high-power lamps, especially HBO mercury burners, where ordinary glass does not withstand the high temperature.

Filters 32 mm in diameter can be used in most microscopes from many manufacturers. In Carl Zeiss Jena microscopes they can be used, among others, in the following fluorescence microscopes:
- Carl Zeiss Jena Jenamed Fluorescence (Fluoreszenz)
- Carl Zeiss Jena Jenalumar
- Carl Zeiss Jena Fluoval / Fluoval-2
- Carl Zeiss Jena Laboval Fluorescence

In the excitation path this filter does not work on its own. It is added to the main filter to cut off the red that filter passes and to narrow the excitation band further.

Description of this part for the microscope.

Carl Zeiss Jena
Model: B229g
Filter type: red-absorbing glass filter (the manufacturer calls it Rotsperrfilter)
Diameter: 32 mm
Colour: blue
Toughened version: yes

The "g" at the end of the designation stands for the German gehärtet, meaning toughened. The manufacturer gave it to filters meant to work right next to high-power lamps, especially HBO mercury burners, where ordinary glass does not withstand the high temperature.

Filters 32 mm in diameter can be used in most microscopes from many manufacturers. In Carl Zeiss Jena microscopes they can be used, among others, in the following fluorescence microscopes:
- Carl Zeiss Jena Jenamed Fluorescence (Fluoreszenz)
- Carl Zeiss Jena Jenalumar
- Carl Zeiss Jena Fluoval / Fluoval-2
- Carl Zeiss Jena Laboval Fluorescence

In the excitation path this filter does not work on its own. It is added to the main filter to cut off the red that filter passes and to narrow the excitation band further.

Description of this part for the microscope.

filter:
Manufacturer: Carl Zeiss Jena
Model: U204g
Filter type: glass excitation filter for UV fluorescence (the manufacturer calls it Erregerfilter)
Glass type: UG 1
Diameter: 32 mm
Thickness: 2 mm
Colour: black-violet, passes very little visible light
Toughened version: yes

The g at the end of the designation stands for the German gehärtet, meaning toughened. The manufacturer gave it to filters meant to work right next to high-power lamps, especially HBO mercury burners, where ordinary glass does not withstand the high temperature. With a UV excitation filter this matters especially, because it sits at the hottest point of the whole path and absorbs most of the burner energy.

Filters 32 mm in diameter can be used in most microscopes from many manufacturers. In Carl Zeiss Jena microscopes they can be used, among others, in the following fluorescence microscopes:
- Carl Zeiss Jena Jenamed Fluorescence (Fluoreszenz)
- Carl Zeiss Jena Jenalumar
- Carl Zeiss Jena Fluoval / Fluoval-2
- Carl Zeiss Jena Laboval Fluorescence

The manufacturer catalogue gives the following transmission values for this glass at 2 mm thickness:
- 0.015 at 302 nm
- 0.093 at 312 nm
- 0.435 at 334 nm
- 0.644 at 366 nm

Across the whole visible range, from 405 to 644 nm, transmission is zero. This means the filter works almost entirely on the 366 nm mercury line, exactly where the HBO burner is strongest in the ultraviolet.

One property of this glass is worth knowing: above 700 nm it starts to transmit again, and at 750 nm transmission reaches 0.210. This infrared leak is invisible to the eye, but it heats the specimen and spoils contrast in photographs.

In the excitation path U204 is also combined with a red-absorbing filter. The manufacturer documentation shows such a pair with the B226 filter, which cleans up the excitation band further.

The same UG 1 glass was supplied in two thicknesses, each with its own designation:
- The Carl Zeiss Jena U204 filter is UG 1 glass 2 mm thick
- The Carl Zeiss Jena U205 filter is UG 1 glass 4 mm thick

The thicker U205 cuts more sharply, but passes about a third less energy on the 366 nm line, so the image is noticeably darker.

A safety note: this filter passes ultraviolet, which is invisible and harmful to the eyes. When working with it, never look into the illumination path without a barrier filter in place.