Home page of the microscope service parts, repair and spare parts online catalog - Sermik-Oplatek Mikroskopservice Dresden f.n.n.Z

Description of this part for the microscope.

rare on the used microscope market. It can be used with microscopes such as:
- Carl Zeiss Jena Jenamed Fluorescence (Fluoreszenz)
- Carl Zeiss Jena Jenalumar
- Carl Zeiss Jena Fluoval
- Carl Zeiss Jena Laboval Fluorescence

Description of this part for the microscope.

filter is exceptionally rare on the used microscope market. It can be used with microscopes such as:
- Carl Zeiss Jena Jenamed Fluorescence (Fluoreszenz)
- Carl Zeiss Jena Jenalumar
- Carl Zeiss Jena Fluoval
- Carl Zeiss Jena Laboval Fluorescence

Description of this part for the microscope.

and other fluorescence microscopes from this company.

Description of this part for the microscope.

Description of this part for the microscope.

a glass frosted filter (so-called ground glass) is located in the optical light beam path. It is mounted on a steel rod ending with a handle positioned outside the microscope base - at the rear, next to the place where the illuminator is inserted. This handle is used to insert and withdraw the frosted filter from the illumination beam path.

This design often leads to damage:
- the glass frosted filter is mounted on the rod in such a way that the glass frequently cracks
- the handle located at the rear of the microscope base very often breaks or crumbles
- the metal rod often jams and as a result of attempts to move it - bends

Here I present and offer this exact frosted filter with the complete movement mechanism:
- glass frosted filter (ground glass)
- holder for mounting the filter to the rod
- all necessary screws to attach this set to the microscope base
- plastic control knob for operating the ground glass

Description of this part for the microscope.

rare on the used microscope market. It can be used with microscopes such as:
- Carl Zeiss Jena Jenamed Fluorescence (Fluoreszenz)
- Carl Zeiss Jena Jenalumar
- Carl Zeiss Jena Fluoval
- Carl Zeiss Jena Laboval Fluorescence

Description of this part for the microscope.

rare on the used microscope market. It can be used with microscopes such as:
- Carl Zeiss Jena Jenamed Fluorescence (Fluoreszenz)
- Carl Zeiss Jena Jenalumar
- Carl Zeiss Jena Fluoval
- Carl Zeiss Jena Laboval Fluorescence

Description of this part for the microscope.

rare on the used microscope market. It can be used with microscopes such as:
- Carl Zeiss Jena Jenamed Fluorescence (Fluoreszenz)
- Carl Zeiss Jena Jenalumar
- Carl Zeiss Jena Fluoval
- Carl Zeiss Jena Laboval Fluorescence

Description of this part for the microscope.

filter holder of Carl Zeiss Jena Vertival microscopes.

Description of this part for the microscope.

catalog number 451495-0100 (45 14 59 - 0100).

The basic versions of the Zeiss Axioskop 20 microscope, intended only for transmitted light operation, do not include any filter cube. In such cases, this type of blanking cover is required to prevent dust from entering the optics.

Description of this part for the microscope.

25).

This is a four-position fluorescence reflector / fluorescence slider / filter cube carrier used in fluorescence microscopy. The element contains four positions for fluorescence filter cubes or fluorescence filter sets.

The offered element contains the following fluorescence filter cubes:

I.
- Excitation: G365
- Beam splitter: FT395
- Barrier filter: LP420

This filter set is typically used for UV fluorescence applications including:
- DAPI
- Hoechst
- AMCA
- DyLight 350
- other UV excited fluorophores

II.
- Excitation: BP450-490
- Beam splitter: FT510
- Barrier filter: LP515

This filter set is typically used for:
- FITC
- GFP
- Fluorescein
- Alexa Fluor 488
- green fluorescence dyes

III.
- empty slot without filter cube

The free slot allows:
- installation of an additional filter set
- operation without fluorescence filters
- faster switching between fluorescence techniques

IV.
- Excitation: BP546
- Beam splitter: FT580
- Barrier filter: LP590

This filter set is typically used for:
- Rhodamine
- TRITC
- Cy3
- Texas Red
- red fluorescence dyes

Such fluorescence filters are commonly used in:
- cell biology
- histology
- cytology
- immunology
- microbiology
- laboratory diagnostics
- molecular biology
- DNA and RNA analysis
- fluorochrome analysis
- observation of fluorescently labelled antibodies
- tissue autofluorescence studies
- observation of chlorophyll and plant specimens
- analysis of FITC, DAPI, AMCA, Rhodamine and related fluorescent dyes
- fluorescence photomicrography
- material science and technical analysis
- observation of UV fluorescent minerals and substances

Such filter sets are also popular among hobbyists and fluorescence microscopy enthusiasts:
- observing insect autofluorescence
- observing UV fluorescent minerals
- fluorescence photography
- observing fungi, pollen and biological structures
- experiments with fluorescent dyes

This fluorescence reflector is compatible among others with the following Carl Zeiss microscopes:
- Carl Zeiss Axioplan
- Carl Zeiss Axioskop
- Carl Zeiss Axiophot
- Carl Zeiss Axiovert
- other Carl Zeiss fluorescence microscopes using the 4FL system

Different names are commonly used for this element, including:
- fluorescence reflector
- fluorescence slider
- fluorescence module
- filter cube carrier
- filter cube holder
- fluorescence turret
- fluorescence filter holder
- fluorescence filter cube
- fluorescence cassette
- fluorescence insert
- fluorescence block

Description of this part for the microscope.

diffuser)
- blue
- yellow
- green

New (usually Chinese) microscope filters are sometimes not even made of glass. And even if they are made of glass - you may encounter scandalous examples where the glass is only surface-colored. Unfortunately, cheap colored glass filters may not be internally homogeneous and can cause light refraction distortions.

This filter set by PZO was originally sold together with the PZO Biolar microscope. It was manufactured in the 1970s (and certainly before 1977), so these are optical glasses that are about half a century old! They have never been used until now (this is so-called NOS - New Old Stock), therefore these are excellent old-class optical glasses of the highest quality.

Filters with a 32 mm diameter are used - and can be used - in microscopes from various manufacturers:
- Carl Zeiss Jena
- Zeiss West Germany
- Eschenbach
- Meopta
- Nikon
- ROW
- Leitz Wetzlar
- Wild
- Hund
- Kosmos
- and many others

Important - popular microscopes by Lomo (for example Lomo Biolam) have a 33 mm filter holder, so the filters shown here have too small a diameter and may fall out from some Lomo holders.

Description of this part for the microscope.

parameters:
Filter colour: blue, matt
Function: NCB - converts the yellow light of the halogen lamp into naturally white light
Mounting diameter: adjustable from 25.5 mm to 26 mm

Mechanically, this filter is mounted on the front end of the halogen illuminator (over the light output opening). Its effect is to produce light with a colour similar to that emitted by a cool white LED.

Description of this part for the microscope.

inside the microscope base. It includes a mechanism that allows these filters to be inserted into or removed from the light path using control buttons. Since more than one filter can be placed in the light path at the same time, the mechanism also includes an additional (seventh) button that removes all filters from the light path simultaneously. Because of this seventh button, some descriptions incorrectly state that the microscope base contains seven filters.

The following filters are installed in this filter cassette:
- NCB filter (blue conversion filter that transforms yellow incandescent lamp light into naturally white light), Zeiss P/N 46 78 50 (yellow stripe on the push button)
- VG 9 green filter, Zeiss P/N 46 78 05 (green stripe on the push button)
- two neutral density filters 0.5, Zeiss P/N 46 78 40 (light grey stripes on the push button)
- neutral density filter 0.12, Zeiss P/N 46 78 41 (grey stripe on the push button)
- neutral density filter 0.03, Zeiss P/N 46 78 42 (dark grey stripe on the push button)

Combinations of the neutral density filters provide stepwise light transmission values ranging from 1.5% to 100%.

Each of these filters can be removed from its holder, resulting in a standard 32 mm round microscope filter that can also be used in microscopes made by other manufacturers. In addition, any other 32 mm round filter can be installed in this filter cassette, allowing the filter set of the microscope to be configured freely.

This filter cassette with its complete control mechanism fits all versions of Carl Zeiss Photomicroscope microscopes (also known as Zeiss PM or Zeiss Phomi) as well as Zeiss Universal microscopes. However, there are also some less common variants of the Zeiss Universal that do not include this filter mechanism inside the microscope base. Importantly, the Carl Zeiss Ultraphot microscope does not use this component.

The last photographs in the gallery indicate with an arrow exactly which component is being offered and where it is located inside the microscope. This allows unambiguous identification of the part.

Description of this part for the microscope.

reflector, performs two functions:
- it contains a barrier filter (emission filter) for the 450 nm wavelength
- it acts as an angular mirror, reflecting the light beam from the EPI fluorescence illuminator vertically downward through the objective toward the specimen.

This fluorescence filter cube fits the following EPI fluorescence microscopes:
- Carl Zeiss Jena Fluoval Fluoreszenz
- Carl Zeiss Jena Fluoval-2 (built on the Carl Zeiss Jena Amplival microscope platform)

Important - this microscope component does not fit the first version of the Carl Zeiss Jena Fluoval microscope. The Fluoval microscope (without "2") does not use a filter cube. Instead, it has a simple permanently mounted swing-out barrier filter.

The filter cube has two positions:
- empty, for normal transmitted-light operation
- 450 nm for reflected-light fluorescence (EPI) operation

Mechanically, this filter cube is installed between the objective nosepiece and the microscope head. It slides into a dedicated slot and moves along a dovetail guide. On the Carl Zeiss Jena Laboval Fluoreszenz microscope, this slot is located in the additional upper horizontal arm of the EPI fluorescence illuminator. On the Carl Zeiss Jena Fluoval-2 microscope, the slot is located in the EPI fluorescence arm, which is an integral part of the microscope. The Fluoval-2 is based on the Carl Zeiss Jena Amplival microscope, which has a modular design. Mechanically, the Fluoval-2 is essentially an Amplival equipped with an upper EPI fluorescence arm.

Sliding the cube along this dovetail guide switches between the empty position (transmitted light) and EPI fluorescence with the 450 nm filter.

Description of this part for the microscope.

This filter is mounted in the sheet-metal housing of the large HBO 250W fluorescence illuminator. This illuminator is part of a large mirror light beam splitter used in Carl Zeiss Jena Jenalumar microscopes. The KG filter is designed to absorb heat (infrared radiation) emitted by the high-pressure HBO mercury lamp.

Please note that there is also a version of the Carl Zeiss Jena Jenalumar microscopes with a small 50W HBO illuminator. This KG filter glass was removed from a large 250W illuminator.

On the used market, in microscopy forums and in microscopy groups, several names are used for this filter, all referring to the same type of filter:
- KG filter / KG glass
- heat filter
- heat absorbing filter
- heat absorption filter
- heat-absorbing glass
- heat absorption glass
- absorption filter
- IR filter
- infrared blocking filter
- infrared cut filter
- KG3 heat absorption filter

This microscope element is highly susceptible to damage. When this happens, the filter needs to be replaced, for example with the filter presented here. Here are examples of damage that can occur during use of the fluorescence illuminator and reasons why replacement of the filter may be necessary:
- the KG filter may crack or become cloudy due to overheating
- the filter may also chip or break as a result of mechanical damage, for example during transport
- the filter may sometimes be damaged during improper maintenance

High-performance heat filters such as this KG filter used with a 250W lamp are very often used by people building their own DIY projects. The housing with screw mounting makes this particularly convenient.

The last photo in the gallery shows the housing of the HBO 250W illuminator (lampenhaus), with an arrow indicating which element is being described and where it is located inside the illuminator. This makes it possible to identify the element unambiguously.

Description of this part for the microscope.

Photomicroscope microscopes.

In the base of Zeiss Universal / Zeiss Photomicroscope class microscopes there is a set of lenses that collimate the light beam. Together they form the collector of the Köhler illumination, even though they are not mechanically connected to each other.

Here I present a module consisting of:
- the second collector lens in the light path
- the heat filter (heat absorbing KG filter)
- the metal mount holding these two glass elements
- the screws used to fasten this mount with the lens and filter to the microscope base

The last photos in the gallery show the complete base of a Zeiss Photomicroscope III microscope, with an arrow indicating which element this is and exactly where it is located, which allows unambiguous identification of this part.

This lens-filter assembly fits the bases of the following microscope models:
- Zeiss Universal
- Zeiss Photomicroscope I
- Zeiss Photomicroscope II
- Zeiss Photomicroscope III / III RS

This is a service part for those who need to replace this component in their microscope. It is not a complete illumination collector.

Replacing this part can remedy, among others, the following problems:
- the KG filter is cracked or chipped, which often happens due to overheating or a shock during transport
- the collimator lens (i.e. the second lens in the Köhler system) is cracked, chipped or damaged by fungus
- one of the metal parts is bent or broken: the filter mount, the lens mount or the mount to the microscope base

Over the years, Carl Zeiss microscopes of the Photomicroscope series have acquired various names that users commonly call them by. These include, among others:
- Zeiss Photomikroskop
- PM, e.g. Zeiss PM II or Zeiss PM III
- Zeiss Phomi, e.g. Zeiss Phomi II
It should also be mentioned that Zeiss Photomicroscope microscopes were also sold under the Opton brand.

Description of this part for the microscope.

catalogue number: 46 78 36 (also written as 467836)
Colour: blue matt
Diameter: 32 mm
Material: glass

This is a filter that combines the functions of a blue filter and a ground glass diffuser:
- the blue filter is used to convert the yellow, warm light of the bulb into light closer to white light
- the matt filter (called a diffuser or ground glass) is used to even out the light across the whole area of the specimen and to remove the visible filament of the bulb

This does, however, have one consequence: without a visible filament it is not possible to work with illumination set up according to the Koehler method - and Zeiss mentions this in the leaflet supplied with the filter.

Very often only one or two microscope filters can be fitted in a microscope, for example one on the illuminator and the second in the holder below the condenser. The fact that the glass filter offered here is a 2-in-1 solution makes it possible to save the possible second slot for another filter.

The diameter of 32 mm is a standard with many manufacturers, which means this filter can be used with microscopes of numerous brands. The "compatibility" section lists over a dozen such microscopes, but it is not a complete list.

Description of this part for the microscope.

Carl Zeiss Jena
Model: KP425
Type: interference excitation filter for fluorescence
Diameter: 32 mm
Mirror coating: yes

Filters 32 mm in diameter can be used in most microscopes from many manufacturers. In Carl Zeiss Jena microscopes they can be used, among others, in the following fluorescence microscopes:
- Carl Zeiss Jena Jenamed Fluorescence (Fluoreszenz)
- Carl Zeiss Jena Jenalumar
- Carl Zeiss Jena Fluoval
- Carl Zeiss Jena Laboval Fluorescence

Note - because this is an interference filter (it has a mirrored surface), the angle at which the filter sits relative to the incoming light matters and affects its parameters.

The coating on interference filters is sensitive not only to mechanical damage, for example scratches from improper storage or cleaning. Several decades of work right next to the HBO lamp can cloud it or make it delaminate, which shows as patches or rainbow islands that do not disappear when you rotate it. In that case the best solution is to replace the filter with one like the one I offer here.

Description of this part for the microscope.

Carl Zeiss Jena
Model: KP490
Type: interference excitation filter for fluorescence
Diameter: 32 mm
Mirror coating: yes

Filters 32 mm in diameter can be used in most microscopes from many manufacturers. In Carl Zeiss Jena microscopes they can be used, among others, in the following fluorescence microscopes:
- Carl Zeiss Jena Jenamed Fluorescence (Fluoreszenz)
- Carl Zeiss Jena Jenalumar
- Carl Zeiss Jena Fluoval
- Carl Zeiss Jena Laboval Fluorescence

Note - this is an interference filter, which you can tell from the mirrored surface. For that reason the angle at which the filter sits relative to the incoming light matters and affects its parameters.

The coating on interference filters is sensitive to mechanical damage, for example scratches from improper storage or cleaning. Age is a separate matter - several decades of work right next to the HBO lamp can cloud it or make it delaminate, which shows as patches or rainbow islands that do not disappear when you rotate it. In that case the best solution is to replace the filter with one like the one I offer here.

Description of this part for the microscope.

Carl Zeiss Jena
Model: KP560
Type: interference excitation filter for fluorescence
Diameter: 32 mm
Mirror coating: yes

Filters 32 mm in diameter can be used in most microscopes from many manufacturers. In Carl Zeiss Jena microscopes they can be used, among others, in the following fluorescence microscopes:
- Carl Zeiss Jena Jenamed Fluorescence (Fluoreszenz)
- Carl Zeiss Jena Jenalumar
- Carl Zeiss Jena Fluoval
- Carl Zeiss Jena Laboval Fluorescence

Note - the mirrored surface gives away that this is an interference filter. With filters of this kind the angle to the incoming light is not indifferent and affects their parameters.

The coating on interference filters is sensitive to mechanical damage, for example scratches from improper storage or cleaning. The second enemy is time - several decades of work right next to the HBO lamp can cloud it or make it delaminate, which shows as patches or rainbow islands that do not disappear when you rotate it. In that case the best solution is to replace the filter with one like the one I offer here.

Description of this part for the microscope.

Carl Zeiss Jena
Model: B226g
Filter type: red-absorbing glass filter (the manufacturer calls it Rotsperrfilter)
Diameter: 32 mm
Colour: blue
Toughened version: yes

The letter "g" at the end of the designation is short for the German gehärtet, meaning toughened - the manufacturer gave it to filters meant to work right next to high-power lamps, especially HBO mercury burners, where ordinary glass does not withstand the high temperature.

Filters 32 mm in diameter can be used in most microscopes from many manufacturers. In Carl Zeiss Jena microscopes they can be used, among others, in the following fluorescence microscopes:
- Carl Zeiss Jena Jenamed Fluorescence (Fluoreszenz)
- Carl Zeiss Jena Jenalumar
- Carl Zeiss Jena Fluoval / Fluoval-2
- Carl Zeiss Jena Laboval Fluorescence

In the excitation path this filter does not work on its own. It is added to the main filter to cut off the red passed by that filter and to narrow the excitation band further.

Description of this part for the microscope.

Carl Zeiss Jena
Model: B228g
Filter type: red-absorbing glass filter (the manufacturer calls it Rotsperrfilter)
Diameter: 32 mm
Colour: blue
Toughened version: yes

The "g" at the end of the designation stands for the German gehärtet, meaning toughened. The manufacturer gave it to filters meant to work right next to high-power lamps, especially HBO mercury burners, where ordinary glass does not withstand the high temperature.

Filters 32 mm in diameter can be used in most microscopes from many manufacturers. In Carl Zeiss Jena microscopes they can be used, among others, in the following fluorescence microscopes:
- Carl Zeiss Jena Jenamed Fluorescence (Fluoreszenz)
- Carl Zeiss Jena Jenalumar
- Carl Zeiss Jena Fluoval / Fluoval-2
- Carl Zeiss Jena Laboval Fluorescence

In the excitation path this filter does not work on its own. It is added to the main filter to cut off the red that filter passes and to narrow the excitation band further.

Description of this part for the microscope.

Carl Zeiss Jena
Model: B229g
Filter type: red-absorbing glass filter (the manufacturer calls it Rotsperrfilter)
Diameter: 32 mm
Colour: blue
Toughened version: yes

The "g" at the end of the designation stands for the German gehärtet, meaning toughened. The manufacturer gave it to filters meant to work right next to high-power lamps, especially HBO mercury burners, where ordinary glass does not withstand the high temperature.

Filters 32 mm in diameter can be used in most microscopes from many manufacturers. In Carl Zeiss Jena microscopes they can be used, among others, in the following fluorescence microscopes:
- Carl Zeiss Jena Jenamed Fluorescence (Fluoreszenz)
- Carl Zeiss Jena Jenalumar
- Carl Zeiss Jena Fluoval / Fluoval-2
- Carl Zeiss Jena Laboval Fluorescence

In the excitation path this filter does not work on its own. It is added to the main filter to cut off the red that filter passes and to narrow the excitation band further.

Description of this part for the microscope.

filter:
Manufacturer: Carl Zeiss Jena
Model: U204g
Filter type: glass excitation filter for UV fluorescence (the manufacturer calls it Erregerfilter)
Glass type: UG 1
Diameter: 32 mm
Thickness: 2 mm
Colour: black-violet, passes very little visible light
Toughened version: yes

The g at the end of the designation stands for the German gehärtet, meaning toughened. The manufacturer gave it to filters meant to work right next to high-power lamps, especially HBO mercury burners, where ordinary glass does not withstand the high temperature. With a UV excitation filter this matters especially, because it sits at the hottest point of the whole path and absorbs most of the burner energy.

Filters 32 mm in diameter can be used in most microscopes from many manufacturers. In Carl Zeiss Jena microscopes they can be used, among others, in the following fluorescence microscopes:
- Carl Zeiss Jena Jenamed Fluorescence (Fluoreszenz)
- Carl Zeiss Jena Jenalumar
- Carl Zeiss Jena Fluoval / Fluoval-2
- Carl Zeiss Jena Laboval Fluorescence

The manufacturer catalogue gives the following transmission values for this glass at 2 mm thickness:
- 0.015 at 302 nm
- 0.093 at 312 nm
- 0.435 at 334 nm
- 0.644 at 366 nm

Across the whole visible range, from 405 to 644 nm, transmission is zero. This means the filter works almost entirely on the 366 nm mercury line, exactly where the HBO burner is strongest in the ultraviolet.

One property of this glass is worth knowing: above 700 nm it starts to transmit again, and at 750 nm transmission reaches 0.210. This infrared leak is invisible to the eye, but it heats the specimen and spoils contrast in photographs.

In the excitation path U204 is also combined with a red-absorbing filter. The manufacturer documentation shows such a pair with the B226 filter, which cleans up the excitation band further.

The same UG 1 glass was supplied in two thicknesses, each with its own designation:
- The Carl Zeiss Jena U204 filter is UG 1 glass 2 mm thick
- The Carl Zeiss Jena U205 filter is UG 1 glass 4 mm thick

The thicker U205 cuts more sharply, but passes about a third less energy on the 366 nm line, so the image is noticeably darker.

A safety note: this filter passes ultraviolet, which is invisible and harmful to the eyes. When working with it, never look into the illumination path without a barrier filter in place.

Description of this part for the microscope.

following microscopes:
- Carl Zeiss Jena Jenaval
- Carl Zeiss Jena Jenalumar
- Carl Zeiss Jena Jenapol
- Carl Zeiss Jena Jenavert

Each of these discs has 5 seats that take filters of 32 mm diameter. The filters are locked in place by a springy retaining ring (a so-called Sprengring) so that they cannot fall out. The set offered here comes with 10 such rings. Since each wheel can be loaded with five filters, the total number of possible combinations is 25.

At the moment these filter seats are empty. Neither wheel has any filters fitted - you are free to load them with whatever filters you like.

This two-wheel module fits halogen, HBO and XBO lamphouses.

Parts made by Carl Zeiss Jena, cast or injection moulded from ZnAl alloy, carry their own numbers by which they can be identified. This double filter revolver module consists of 3 cast components:
- 2672 Si10 CZ - the frame carrying both wheels. It is at the same time the outer cover plate with which the whole module is screwed onto the lamphouse
- 2957 Si10 CZ - the thick filter wheel
- and note: the thin filter wheel also carries the number 2957 Si10 CZ, which is unusual for this manufacturer - that two parts differing in dimensions should share the same number

This double filter wheel is sometimes also called:
- double filter holder
- double filter turret
- dual filter carrier
- double filter slider
- double filter changer

Here https://www.microscopeparts.eu/part/99693 you can see what a complete 6V 25W halogen illuminator looks like with a single filter wheel fitted. The double set offered here fits such an illuminator among others, so the single filter wheel from the illuminator of a Carl Zeiss Jena Jenaval microscope can, for example, be replaced by the double one.