Home page of the microscope service parts, repair and spare parts online catalog - Sermik-Oplatek Mikroskopservice Dresden f.n.n.Z

Description of this part for the microscope.

supply:
Manufacturer: Carl Zeiss
Model: VWH 200/C 250 f-3b
Manufacturer P/N: 39 26 34 (also written as 392634)
Supported fluorescence lamps: HBO 200 W/4 L1, HBO 200 W/4 L2 and CSI 250W
Input voltage: 220 V – 240 V
Power rating: 900 VA

This power supply was supplied as standard with Zeiss Photomicroscope microscopes (commonly known as the Zeiss Phomi) whenever the microscope was equipped with an HBO high-pressure fluorescence illuminator (also referred to as a burner or lamp).

The same or very similar illuminators with identical electrical specifications were used in, among others:
- Zeiss Standard WL
- Zeiss Photomicroscope
- Zeiss Universal
- Zeiss Ultraphot
- such illuminators were also occasionally adapted for use with other microscope models, including those made by other manufacturers
Therefore, this power supply is suitable for all of the applications listed above.

Please note: the last photograph shows a dent in the housing. This does not affect the operation of the unit. Since the dent is located on the underside of the housing, it is not visible when the power supply is standing on a laboratory bench.

IMPORTANT: Always protect your eyesight when working with UV illumination. Never exceed the recommended service life of HBO high-pressure lamps. Always follow the operating instructions supplied with the fluorescence illuminator.

Description of this part for the microscope.

phase contrast and darkfield) allow a phase contrast condenser to be configured individually. This makes it possible to use exactly the observation techniques and exactly the microscope objectives required.

Basic parameters of this phase annulus:
Manufacturer: Carl Zeiss (Oberkochen, West Germany)
Condenser marking: "2"
For objectives: marked "Ph2", i.e. magnifications from 16x to 40x
Material: glass mounted in a metal retaining ring with flange
Outside diameter of the retaining ring: 22 mm
Phase annulus glass diameter: 16 mm
Height of the retaining ring: 3.5 mm

Important: this microscope component (phase annulus / phase diaphragm) is not intended for any specific microscope model. It is a component used to upgrade or repair a microscope phase contrast condenser. It is not intended for upgrading or repairing one specific condenser model. Many Carl Zeiss condenser models have a suitable opening into which this phase annulus can be inserted.

This particular phase annulus was removed from a fluorescence phase contrast condenser — an older version of the Zeiss condenser P/N 46 52 78. Before installing it in your condenser, carefully compare the shape of its housing and the dimensions of the retaining ring.

The last photograph in the gallery shows the turret disc of a Zeiss phase contrast condenser. An arrow indicates this very phase annulus — the one offered here — already installed.

Description of this part for the microscope.

condensers.

Basic parameters of this clear glass insert:
Manufacturer: Carl Zeiss (Oberkochen, West Germany)
Material: glass mounted in a metal retaining ring with flange
Outside diameter of the retaining ring: 22 mm
Diameter of the clear glass disc: 16 mm
Height of the retaining ring: 3.5 mm

Below are some example applications for this clear glass blank. It can be printed or fitted with, among others:
- a phase annulus or darkfield annulus matching the original one that was installed in the condenser but has become damaged
- an annulus missing from the condenser
- coloured Rheinberg rings
- oblique illumination diaphragms producing a DIC-like effect
- a Hoffmann modulation contrast pattern
- phase annuli for objectives made by other manufacturers, for example Carl Zeiss Jena PhV
- any completely custom DIY design

This particular clear glass ring insert was removed from a fluorescence phase contrast condenser — an older version of the Zeiss condenser P/N 46 52 78. Before installing it in your condenser, carefully compare the shape of its housing and the dimensions of the retaining ring.

Interchangeable diaphragm carriers (whether for phase contrast or, for example, darkfield) make it possible to configure a phase contrast condenser exactly as required. This allows the condenser to support precisely the observation techniques and microscope objectives needed.

The clear glass blank presented here is not a component intended for any specific microscope model. Instead, it is used to upgrade or repair a microscope phase contrast condenser. Likewise, it is not dedicated to any specific condenser model. Many Carl Zeiss condenser models have a suitable opening into which this complete insert can be fitted and customised with your own pattern.

The last photograph in the gallery shows the turret disc of a Zeiss phase contrast condenser. An arrow indicates the clear glass ring insert offered here, already installed.

Description of this part for the microscope.

applications.

Replaceable diaphragms (both for phase contrast and darkfield) for phase contrast condensers allow the condenser to be configured individually. Thanks to this, it can support exactly the observation techniques and exactly the microscope objectives that are required.

Important: by installing the fluorescence-polarization diaphragm presented here, a condenser currently equipped with standard phase contrast diaphragms can be upgraded with this unique function. A detailed description of the capabilities offered by this technique is provided below.

Basic parameters of this phase contrast diaphragm:
Manufacturer: Carl Zeiss (Oberkochen, West Germany)
Condenser marking: "2". On the Zeiss condenser P/N 46 52 78 this position is marked in blue
For microscope objectives: to obtain phase contrast operation, use microscope objectives marked "Ph2", i.e. objectives with magnifications from 16x to 40x
Substrate material: glass mounted in a metal holder with a flange
Ring material: polarizing film
Outside diameter of the retaining ring: 22 mm
Glass disc diameter: 16 mm
Retaining ring height: 3.5 mm

Important: this component (phase contrast annulus / phase contrast diaphragm) is not intended for a specific microscope model. It is a component for upgrading or repairing a microscope phase contrast condenser. It is not intended for upgrading or repairing any specific condenser model. Many Carl Zeiss condenser models have a matching opening into which this phase contrast annulus can be installed.

In the Carl Zeiss condenser P/N 46 52 78, the phase annuli in positions 2 and 3 are not ordinary openings in an opaque mask but openings in a polarizing film. The polarization direction of these annuli is N-S, so the direct light beam that forms the phase contrast image is linearly polarized. Since it is polarized, its intensity can be adjusted continuously with a second polarizer – without any mechanical parts, diaphragms or shutters.

This second polarizer can be any pair of rotatable polarizing filters. In the original Zeiss design, this is the Carl Zeiss light control unit P/N 47 72 20 with two independently rotatable polarizers mounted beneath the condenser. This makes it possible to switch on phase contrast while simultaneously reducing the illumination intensity to a safe level.

- when the polarizer is aligned parallel to the N-S axis of the annulus – full transmission, maximum phase contrast beam, full-strength phase contrast image;
- when the polarizer is crossed (E-W) – the annulus "goes dark", the objective phase plate no longer receives the direct beam, and the phase contrast image disappears;
- when the polarizer is set to intermediate positions – smooth adjustment of the contribution of the phase contrast image.

When the illumination source is a fluorescence lamp (or, of course, an LED FL light source), simply rotating the polarizer beneath the condenser (together with removing the excitation filter) allows the user to switch from a fluorescence image to a conventional phase contrast image. With the polarizer crossed and the excitation filter installed, the phase annulus is extinguished and only pure fluorescence is visible; as the polarizer is rotated, the phase contrast image gradually increases and is superimposed on the fluorescence image. The second polarizer in unit P/N 47 72 20 is additionally used to attenuate the overall illumination intensity (to protect the specimen or to control the specimen's exposure to fluorescence light).

And now an interesting note from the Ultraphot-shop website https://www.microscopeparts.eu/(http://www.the-ultraphot-shop.org.uk/bicolor.htm), © Spike Walker 2007:

Because the annulus is a linear N-S polarizer, it can be used to produce coloured phase contrast: when a two-colour polarizing filter (Cokin type) is placed between the microscope polarizer and the condenser annulus, the specimen takes on the colour of the illuminating annulus against the background colour of the remaining part of the disc – for example, an orange specimen on a green background when using a red-green filter.

This particular phase contrast annulus was removed from a fluorescence phase contrast condenser – an older version of the Zeiss condenser P/N 46 52 78. Before deciding to install it in your condenser, carefully compare the shape of its housing and the dimensions of the retaining ring.

The last photograph in the gallery shows the turret from a Zeiss phase contrast condenser. The arrow indicates the phase contrast annulus offered here, already installed.

Description of this part for the microscope.

applications.

Replaceable diaphragms (both for phase contrast and darkfield) for phase contrast condensers allow the condenser to be configured individually. Thanks to this, it can support exactly the observation techniques and exactly the microscope objectives that are required.

Important: by installing the fluorescence-polarization diaphragm presented here, a condenser currently equipped with standard phase contrast diaphragms can be upgraded with this unique function. A detailed description of the capabilities offered by this technique is provided below.

Basic parameters of this phase contrast diaphragm:
Manufacturer: Carl Zeiss (Oberkochen, West Germany)
Condenser marking: "3". On the Zeiss condenser P/N 46 52 78 this position is marked in blue
For microscope objectives: to obtain phase contrast operation, use microscope objectives marked "Ph3", i.e. objectives with magnifications from 40x to 100x
Substrate material: glass mounted in a metal holder with a flange
Ring material: polarizing film
Outside diameter of the retaining ring: 28 mm
Retaining ring height: 6 mm

Important: this component (phase contrast annulus / phase contrast diaphragm) is not intended for a specific microscope model. It is a component for upgrading or repairing a microscope phase contrast condenser. It is not intended for upgrading or repairing any specific condenser model. Many Carl Zeiss condenser models have a matching opening into which this phase contrast annulus can be installed.

In the Carl Zeiss condenser P/N 46 52 78, the phase annuli in positions 2 and 3 are not ordinary openings in an opaque mask but openings in a polarizing film. The polarization direction of these annuli is N-S, so the direct light beam that forms the phase contrast image is linearly polarized. Since it is polarized, its intensity can be adjusted continuously with a second polarizer – without any mechanical parts, diaphragms or shutters.

This second polarizer can be any pair of rotatable polarizing filters. In the original Zeiss design, this is the Carl Zeiss light control unit P/N 47 72 20 with two independently rotatable polarizers mounted beneath the condenser. This makes it possible to switch on phase contrast while simultaneously reducing the illumination intensity to a safe level.

- when the polarizer is aligned parallel to the N-S axis of the annulus – full transmission, maximum phase contrast beam, full-strength phase contrast image;
- when the polarizer is crossed (E-W) – the annulus "goes dark", the objective phase plate no longer receives the direct beam, and the phase contrast image disappears;
- when the polarizer is set to intermediate positions – smooth adjustment of the contribution of the phase contrast image.

When the illumination source is a fluorescence lamp (or, of course, an LED FL light source), simply rotating the polarizer beneath the condenser (together with removing the excitation filter) allows the user to switch from a fluorescence image to a conventional phase contrast image. With the polarizer crossed and the excitation filter installed, the phase annulus is extinguished and only pure fluorescence is visible; as the polarizer is rotated, the phase contrast image gradually increases and is superimposed on the fluorescence image. The second polarizer in unit P/N 47 72 20 is additionally used to attenuate the overall illumination intensity (to protect the specimen or to control the specimen's exposure to fluorescence light).

And now an interesting note from the Ultraphot-shop website https://www.microscopeparts.eu/(http://www.the-ultraphot-shop.org.uk/bicolor.htm), © Spike Walker 2007:

Because the annulus is a linear N-S polarizer, it can be used to produce coloured phase contrast: when a two-colour polarizing filter (Cokin type) is placed between the microscope polarizer and the condenser annulus, the specimen takes on the colour of the illuminating annulus against the background colour of the remaining part of the disc – for example, an orange specimen on a green background when using a red-green filter.

This particular phase contrast annulus was removed from a fluorescence phase contrast condenser – an older version of the Zeiss condenser P/N 46 52 78. Before deciding to install it in your condenser, carefully compare the shape of its housing and the dimensions of the retaining ring.

The last photograph in the gallery shows the turret from a Zeiss phase contrast condenser. The arrow indicates the phase contrast annulus offered here, already installed.

Description of this part for the microscope.

47 20 03 - 9906 (alternative notation: 472003).

Several names are used for this microscope model, including:
- Carl Zeiss Photomicroscope III
- Carl Zeiss Phomi III
- Carl Zeiss PM III
- Zeiss Photomicroscope III RS
- Zeiss Phomi 3
- Zeiss PM3 RS

The photographs in the gallery include an arrow indicating exactly which component is being offered and where it is located inside the microscope. This makes it possible to identify the component unambiguously.

Description of this part for the microscope.

in the microscope base. In the operating manual (available here: https://www.science-info.net/docs/zeiss/Photomicroscope3-Transmitted-Light.pdf) this component is identified as item 27 (figure on page 4, description on page 5).

Several names are used for this microscope model, including:
- Carl Zeiss Photomicroscope III
- Carl Zeiss Phomi III
- Carl Zeiss PM III
- Zeiss Photomicroscope III RS
- Zeiss Phomi 3
- Zeiss PM3 RS

The photographs in the gallery include an arrow indicating exactly which component is being offered and where it is located inside the microscope. This makes it possible to identify the component unambiguously.

Description of this part for the microscope.

be Zeiss catalog number P/N 25 444

Several names are used for this microscope model, including:
- Carl Zeiss Photomicroscope III
- Carl Zeiss Phomi III
- Carl Zeiss PM III
- Zeiss Photomicroscope III RS
- Zeiss Phomi 3
- Zeiss PM3 RS

The photographs in the gallery include an arrow indicating exactly which component is being offered and where it is located inside the microscope. This makes it possible to identify the component unambiguously.

Description of this part for the microscope.

hand side of the microscope base. In the operating manual (available here: https://www.science-info.net/docs/zeiss/Photomicroscope3-Transmitted-Light.pdf) they are identified as items 18 and 30 (figure on page 4).

According to the operating manual, the "!" button "has several functions", while the "A" button is described as "camera shutter ready for photography".

Several names are used for this microscope model, including:
- Carl Zeiss Photomicroscope III
- Carl Zeiss Phomi III
- Carl Zeiss PM III
- Zeiss Photomicroscope III RS
- Zeiss Phomi 3
- Zeiss PM3 RS

The photographs in the gallery include an arrow indicating exactly which component is being offered and where it is located inside the microscope. This makes it possible to identify the component unambiguously.

Description of this part for the microscope.

left-hand side of the microscope base. In the operating manual (available here: https://www.science-info.net/docs/zeiss/Photomicroscope3-Transmitted-Light.pdf) they are identified as items 14 and 15 (figure on page 4).

According to the operating manual, the "B" button opens the camera shutter electrically for as long as it is pressed. Releasing the button closes the shutter.

The "T" button opens the camera shutter and keeps it open. Pressing it again releases the mechanism and closes the shutter.

Several names are used for this microscope model, including:
- Carl Zeiss Photomicroscope III
- Carl Zeiss Phomi III
- Carl Zeiss PM III
- Zeiss Photomicroscope III RS
- Zeiss Phomi 3
- Zeiss PM3 RS

The photographs in the gallery include an arrow indicating exactly which component is being offered and where it is located inside the microscope. This makes it possible to identify the component unambiguously.

Description of this part for the microscope.

manual (available here: https://www.science-info.net/docs/zeiss/Photomicroscope3-Transmitted-Light.pdf) it is identified as item 13 (figure on page 4).

The indicator displays the supply voltage of the microscope illumination. The scale provides readings in the ranges of 0–12 V and 0–6 V.

Several names are used for this microscope model, including:
- Carl Zeiss Photomicroscope III
- Carl Zeiss Phomi III
- Carl Zeiss PM III
- Zeiss Photomicroscope III RS
- Zeiss Phomi 3
- Zeiss PM3 RS

The photographs in the gallery include an arrow indicating exactly which component is being offered and where it is located inside the microscope. This makes it possible to identify the component unambiguously.

Description of this part for the microscope.

In the operating manual (available here: https://www.science-info.net/docs/zeiss/Photomicroscope3-Transmitted-Light.pdf) it is identified as item 16 (figure on page 4).

According to the manufacturer's operating manual, this is a brightness indicator used during photomicrography.

Several names are used for this microscope model, including:
- Carl Zeiss Photomicroscope III
- Carl Zeiss Phomi III
- Carl Zeiss PM III
- Zeiss Photomicroscope III RS
- Zeiss Phomi 3
- Zeiss PM3 RS

The photographs in the gallery include an arrow indicating exactly which component is being offered and where it is located inside the microscope. This makes it possible to identify the component unambiguously.

Description of this part for the microscope.

In the operating manual (available here: https://www.science-info.net/docs/zeiss/Photomicroscope3-Transmitted-Light.pdf) it is identified as item 17 (illustration on page 4).

According to the manufacturer's manual, this microscope component is used to adapt the automatic exposure system to the sensitivity of the film in use. The selector adjusts the setting in 3 DIN increments (from DIN 6 to DIN 39) or doubles the ASA values (from ASA 3.2 to ASA 6300).

Several names are used for this microscope model, including:
- Carl Zeiss Photomicroscope III
- Carl Zeiss Phomi III
- Carl Zeiss PM III
- Zeiss Photomicroscope III RS
- Zeiss Phomi 3
- Zeiss PM3 RS

The photographs in the gallery include an arrow indicating exactly which component is being offered and where it is located on the microscope. This allows the part to be identified unambiguously.