Home page of the microscope service parts, repair and spare parts online catalog - Sermik-Oplatek Mikroskopservice Dresden f.n.n.Z

Description of this part for the microscope.

supply:
Manufacturer: Carl Zeiss
Model: VWH 200/C 250 f-3b
Manufacturer P/N: 39 26 34 (also written as 392634)
Supported fluorescence lamps: HBO 200 W/4 L1, HBO 200 W/4 L2 and CSI 250W
Input voltage: 220 V – 240 V
Power rating: 900 VA

This power supply was supplied as standard with Zeiss Photomicroscope microscopes (commonly known as the Zeiss Phomi) whenever the microscope was equipped with an HBO high-pressure fluorescence illuminator (also referred to as a burner or lamp).

The same or very similar illuminators with identical electrical specifications were used in, among others:
- Zeiss Standard WL
- Zeiss Photomicroscope
- Zeiss Universal
- Zeiss Ultraphot
- such illuminators were also occasionally adapted for use with other microscope models, including those made by other manufacturers
Therefore, this power supply is suitable for all of the applications listed above.

Please note: the last photograph shows a dent in the housing. This does not affect the operation of the unit. Since the dent is located on the underside of the housing, it is not visible when the power supply is standing on a laboratory bench.

IMPORTANT: Always protect your eyesight when working with UV illumination. Never exceed the recommended service life of HBO high-pressure lamps. Always follow the operating instructions supplied with the fluorescence illuminator.

Description of this part for the microscope.

phase contrast and darkfield) allow a phase contrast condenser to be configured individually. This makes it possible to use exactly the observation techniques and exactly the microscope objectives required.

Basic parameters of this phase annulus:
Manufacturer: Carl Zeiss (Oberkochen, West Germany)
Condenser marking: "2"
For objectives: marked "Ph2", i.e. magnifications from 16x to 40x
Material: glass mounted in a metal retaining ring with flange
Outside diameter of the retaining ring: 22 mm
Phase annulus glass diameter: 16 mm
Height of the retaining ring: 3.5 mm

Important: this microscope component (phase annulus / phase diaphragm) is not intended for any specific microscope model. It is a component used to upgrade or repair a microscope phase contrast condenser. It is not intended for upgrading or repairing one specific condenser model. Many Carl Zeiss condenser models have a suitable opening into which this phase annulus can be inserted.

This particular phase annulus was removed from a fluorescence phase contrast condenser — an older version of the Zeiss condenser P/N 46 52 78. Before installing it in your condenser, carefully compare the shape of its housing and the dimensions of the retaining ring.

The last photograph in the gallery shows the turret disc of a Zeiss phase contrast condenser. An arrow indicates this very phase annulus — the one offered here — already installed.

Description of this part for the microscope.

condensers.

Basic parameters of this clear glass insert:
Manufacturer: Carl Zeiss (Oberkochen, West Germany)
Material: glass mounted in a metal retaining ring with flange
Outside diameter of the retaining ring: 22 mm
Diameter of the clear glass disc: 16 mm
Height of the retaining ring: 3.5 mm

Below are some example applications for this clear glass blank. It can be printed or fitted with, among others:
- a phase annulus or darkfield annulus matching the original one that was installed in the condenser but has become damaged
- an annulus missing from the condenser
- coloured Rheinberg rings
- oblique illumination diaphragms producing a DIC-like effect
- a Hoffmann modulation contrast pattern
- phase annuli for objectives made by other manufacturers, for example Carl Zeiss Jena PhV
- any completely custom DIY design

This particular clear glass ring insert was removed from a fluorescence phase contrast condenser — an older version of the Zeiss condenser P/N 46 52 78. Before installing it in your condenser, carefully compare the shape of its housing and the dimensions of the retaining ring.

Interchangeable diaphragm carriers (whether for phase contrast or, for example, darkfield) make it possible to configure a phase contrast condenser exactly as required. This allows the condenser to support precisely the observation techniques and microscope objectives needed.

The clear glass blank presented here is not a component intended for any specific microscope model. Instead, it is used to upgrade or repair a microscope phase contrast condenser. Likewise, it is not dedicated to any specific condenser model. Many Carl Zeiss condenser models have a suitable opening into which this complete insert can be fitted and customised with your own pattern.

The last photograph in the gallery shows the turret disc of a Zeiss phase contrast condenser. An arrow indicates the clear glass ring insert offered here, already installed.

Description of this part for the microscope.

applications.

Replaceable diaphragms (both for phase contrast and darkfield) for phase contrast condensers allow the condenser to be configured individually. Thanks to this, it can support exactly the observation techniques and exactly the microscope objectives that are required.

Important: by installing the fluorescence-polarization diaphragm presented here, a condenser currently equipped with standard phase contrast diaphragms can be upgraded with this unique function. A detailed description of the capabilities offered by this technique is provided below.

Basic parameters of this phase contrast diaphragm:
Manufacturer: Carl Zeiss (Oberkochen, West Germany)
Condenser marking: "2". On the Zeiss condenser P/N 46 52 78 this position is marked in blue
For microscope objectives: to obtain phase contrast operation, use microscope objectives marked "Ph2", i.e. objectives with magnifications from 16x to 40x
Substrate material: glass mounted in a metal holder with a flange
Ring material: polarizing film
Outside diameter of the retaining ring: 22 mm
Glass disc diameter: 16 mm
Retaining ring height: 3.5 mm

Important: this component (phase contrast annulus / phase contrast diaphragm) is not intended for a specific microscope model. It is a component for upgrading or repairing a microscope phase contrast condenser. It is not intended for upgrading or repairing any specific condenser model. Many Carl Zeiss condenser models have a matching opening into which this phase contrast annulus can be installed.

In the Carl Zeiss condenser P/N 46 52 78, the phase annuli in positions 2 and 3 are not ordinary openings in an opaque mask but openings in a polarizing film. The polarization direction of these annuli is N-S, so the direct light beam that forms the phase contrast image is linearly polarized. Since it is polarized, its intensity can be adjusted continuously with a second polarizer – without any mechanical parts, diaphragms or shutters.

This second polarizer can be any pair of rotatable polarizing filters. In the original Zeiss design, this is the Carl Zeiss light control unit P/N 47 72 20 with two independently rotatable polarizers mounted beneath the condenser. This makes it possible to switch on phase contrast while simultaneously reducing the illumination intensity to a safe level.

- when the polarizer is aligned parallel to the N-S axis of the annulus – full transmission, maximum phase contrast beam, full-strength phase contrast image;
- when the polarizer is crossed (E-W) – the annulus "goes dark", the objective phase plate no longer receives the direct beam, and the phase contrast image disappears;
- when the polarizer is set to intermediate positions – smooth adjustment of the contribution of the phase contrast image.

When the illumination source is a fluorescence lamp (or, of course, an LED FL light source), simply rotating the polarizer beneath the condenser (together with removing the excitation filter) allows the user to switch from a fluorescence image to a conventional phase contrast image. With the polarizer crossed and the excitation filter installed, the phase annulus is extinguished and only pure fluorescence is visible; as the polarizer is rotated, the phase contrast image gradually increases and is superimposed on the fluorescence image. The second polarizer in unit P/N 47 72 20 is additionally used to attenuate the overall illumination intensity (to protect the specimen or to control the specimen's exposure to fluorescence light).

And now an interesting note from the Ultraphot-shop website https://www.microscopeparts.eu/(http://www.the-ultraphot-shop.org.uk/bicolor.htm), © Spike Walker 2007:

Because the annulus is a linear N-S polarizer, it can be used to produce coloured phase contrast: when a two-colour polarizing filter (Cokin type) is placed between the microscope polarizer and the condenser annulus, the specimen takes on the colour of the illuminating annulus against the background colour of the remaining part of the disc – for example, an orange specimen on a green background when using a red-green filter.

This particular phase contrast annulus was removed from a fluorescence phase contrast condenser – an older version of the Zeiss condenser P/N 46 52 78. Before deciding to install it in your condenser, carefully compare the shape of its housing and the dimensions of the retaining ring.

The last photograph in the gallery shows the turret from a Zeiss phase contrast condenser. The arrow indicates the phase contrast annulus offered here, already installed.

Description of this part for the microscope.

applications.

Replaceable diaphragms (both for phase contrast and darkfield) for phase contrast condensers allow the condenser to be configured individually. Thanks to this, it can support exactly the observation techniques and exactly the microscope objectives that are required.

Important: by installing the fluorescence-polarization diaphragm presented here, a condenser currently equipped with standard phase contrast diaphragms can be upgraded with this unique function. A detailed description of the capabilities offered by this technique is provided below.

Basic parameters of this phase contrast diaphragm:
Manufacturer: Carl Zeiss (Oberkochen, West Germany)
Condenser marking: "3". On the Zeiss condenser P/N 46 52 78 this position is marked in blue
For microscope objectives: to obtain phase contrast operation, use microscope objectives marked "Ph3", i.e. objectives with magnifications from 40x to 100x
Substrate material: glass mounted in a metal holder with a flange
Ring material: polarizing film
Outside diameter of the retaining ring: 28 mm
Retaining ring height: 6 mm

Important: this component (phase contrast annulus / phase contrast diaphragm) is not intended for a specific microscope model. It is a component for upgrading or repairing a microscope phase contrast condenser. It is not intended for upgrading or repairing any specific condenser model. Many Carl Zeiss condenser models have a matching opening into which this phase contrast annulus can be installed.

In the Carl Zeiss condenser P/N 46 52 78, the phase annuli in positions 2 and 3 are not ordinary openings in an opaque mask but openings in a polarizing film. The polarization direction of these annuli is N-S, so the direct light beam that forms the phase contrast image is linearly polarized. Since it is polarized, its intensity can be adjusted continuously with a second polarizer – without any mechanical parts, diaphragms or shutters.

This second polarizer can be any pair of rotatable polarizing filters. In the original Zeiss design, this is the Carl Zeiss light control unit P/N 47 72 20 with two independently rotatable polarizers mounted beneath the condenser. This makes it possible to switch on phase contrast while simultaneously reducing the illumination intensity to a safe level.

- when the polarizer is aligned parallel to the N-S axis of the annulus – full transmission, maximum phase contrast beam, full-strength phase contrast image;
- when the polarizer is crossed (E-W) – the annulus "goes dark", the objective phase plate no longer receives the direct beam, and the phase contrast image disappears;
- when the polarizer is set to intermediate positions – smooth adjustment of the contribution of the phase contrast image.

When the illumination source is a fluorescence lamp (or, of course, an LED FL light source), simply rotating the polarizer beneath the condenser (together with removing the excitation filter) allows the user to switch from a fluorescence image to a conventional phase contrast image. With the polarizer crossed and the excitation filter installed, the phase annulus is extinguished and only pure fluorescence is visible; as the polarizer is rotated, the phase contrast image gradually increases and is superimposed on the fluorescence image. The second polarizer in unit P/N 47 72 20 is additionally used to attenuate the overall illumination intensity (to protect the specimen or to control the specimen's exposure to fluorescence light).

And now an interesting note from the Ultraphot-shop website https://www.microscopeparts.eu/(http://www.the-ultraphot-shop.org.uk/bicolor.htm), © Spike Walker 2007:

Because the annulus is a linear N-S polarizer, it can be used to produce coloured phase contrast: when a two-colour polarizing filter (Cokin type) is placed between the microscope polarizer and the condenser annulus, the specimen takes on the colour of the illuminating annulus against the background colour of the remaining part of the disc – for example, an orange specimen on a green background when using a red-green filter.

This particular phase contrast annulus was removed from a fluorescence phase contrast condenser – an older version of the Zeiss condenser P/N 46 52 78. Before deciding to install it in your condenser, carefully compare the shape of its housing and the dimensions of the retaining ring.

The last photograph in the gallery shows the turret from a Zeiss phase contrast condenser. The arrow indicates the phase contrast annulus offered here, already installed.

Description of this part for the microscope.

are equipped with a base illuminator using a 6 V 15 W lamp. This can be replaced with:
a) a large 60-watt illuminator using a 12 V 60 W lamp
b) a halogen illuminator

This listing presents the illuminator described in option a), namely the 60 W incandescent lamp version.

Basic specifications of this illuminator:
Manufacturer: Carl Zeiss (Oberkochen, West Germany)
Model: 60-watt illuminator
Lamp voltage: 12 V
Lamp house mounting method: circular dovetail
Cooling: passive. The entire large lamp house also serves as a heat sink, dissipating the heat generated by the 60-watt lamp
Lamp socket centering screws: yes, the lamp socket is equipped with centering screws
Diffuser: yes, inside the lamp house, in front of the lamp condenser lens (the so-called illumination collector), there is a matte diffuser mounted in a flip-out holder

This particular unit was removed from a Carl Zeiss Photomicroscope II. However, it is compatible with most microscopes of the Zeiss Standard series.

This illuminator fits the following microscopes:
- Zeiss Standard 16 / GFL
- Zeiss Standard WL / WL 89
- Zeiss Universal
- Zeiss Photomicroscope
- Zeiss Photomicroscope II
- Zeiss Photomicroscope III RS
- and also the Zeiss IM 35 / ICM 405 inverted microscope

The following is a quotation from the Zeiss Photomicroscope II instruction manual:

60-watt illuminator

Instead of the 6-v. 15-w base illuminator, the 60-watt illuminator may be firmly connected to the microscope stand. For this purpose, the collector tube with lamp condenser, 46 70 50, must be removed after unscrewing the bottom plate and replaced by the connecting tube, 46 70 41 (Fig. 15). The 60-watt illuminator is mounted in the usual manner with the aid of a dovetail ring, like a body tube, for example.

The variable transformer is identical to the one for the base illuminator and supplies voltages from 0 ... 20 volts. When this equipment is used, the red scale of the voltmeter applies.

Several important points should be mentioned:
- if your microscope is currently equipped with the standard 6 V 15 W illumination system, the illuminator mounting adapter must also be replaced (this is described in the instruction manual quoted above)
- the lamp house of this illuminator is finished in grey. Some Carl Zeiss Standard series microscopes have black, cream or hammered-finish housings. This illuminator is mechanically compatible with those models, although the colour may not be an aesthetically perfect match
- power supplies with a rated output of exactly 60 W are virtually unavailable on the second-hand market. In practice, 12 V 100 W power supplies are used to operate this light source
- please note that exactly the same (!) illuminator is also used on the Zeiss IM 35 / ICM 405 inverted microscopes. Please also refer to the description of illuminator No. 00697 in this database. YES – these are exactly the same illuminators. However, that particular unit was removed from an inverted microscope and therefore has its own separate description. You will also find additional photographs there. In the Zeiss IM 35 instruction manual, the lamp socket is referred to as "Lamp socket 60 P/N 46 80 15", while the lamp house is referred to as "Lamp housing with lamp condenser P/N 46 72 27"
- this illuminator can be used for both transmitted-light and reflected-light (EPI) illumination
- if you would like to manufacture your own DIY illuminator mounting adapter because you do not have the original Zeiss part (P/N 46 70 41), the following information may be useful: the circular dovetail on this illuminator has a diameter of 43 mm, which is the same as on many microscope condensers and mono-, binocular and trinocular microscope heads. This means that, for example, a microscope head mounting adapter can be used as the basis for manufacturing a custom DIY illuminator adapter

Important: The photographs show a lamp installed in the lamp socket. This lamp DOES NOT LIGHT because it is burned out. It is shown only so that you can see which lamp model is used in this illuminator.