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Description of this part for the microscope.

rare on the used microscope market. It can be used with microscopes such as:
- Carl Zeiss Jena Jenamed Fluorescence (Fluoreszenz)
- Carl Zeiss Jena Jenalumar
- Carl Zeiss Jena Fluoval
- Carl Zeiss Jena Laboval Fluorescence

Description of this part for the microscope.

filter is exceptionally rare on the used microscope market. It can be used with microscopes such as:
- Carl Zeiss Jena Jenamed Fluorescence (Fluoreszenz)
- Carl Zeiss Jena Jenalumar
- Carl Zeiss Jena Fluoval
- Carl Zeiss Jena Laboval Fluorescence

Description of this part for the microscope.

Description of this part for the microscope.

a glass frosted filter (so-called ground glass) is located in the optical light beam path. It is mounted on a steel rod ending with a handle positioned outside the microscope base - at the rear, next to the place where the illuminator is inserted. This handle is used to insert and withdraw the frosted filter from the illumination beam path.

This design often leads to damage:
- the glass frosted filter is mounted on the rod in such a way that the glass frequently cracks
- the handle located at the rear of the microscope base very often breaks or crumbles
- the metal rod often jams and as a result of attempts to move it - bends

Here I present and offer this exact frosted filter with the complete movement mechanism:
- glass frosted filter (ground glass)
- holder for mounting the filter to the rod
- all necessary screws to attach this set to the microscope base
- plastic control knob for operating the ground glass

Description of this part for the microscope.

rare on the used microscope market. It can be used with microscopes such as:
- Carl Zeiss Jena Jenamed Fluorescence (Fluoreszenz)
- Carl Zeiss Jena Jenalumar
- Carl Zeiss Jena Fluoval
- Carl Zeiss Jena Laboval Fluorescence

Description of this part for the microscope.

rare on the used microscope market. It can be used with microscopes such as:
- Carl Zeiss Jena Jenamed Fluorescence (Fluoreszenz)
- Carl Zeiss Jena Jenalumar
- Carl Zeiss Jena Fluoval
- Carl Zeiss Jena Laboval Fluorescence

Description of this part for the microscope.

rare on the used microscope market. It can be used with microscopes such as:
- Carl Zeiss Jena Jenamed Fluorescence (Fluoreszenz)
- Carl Zeiss Jena Jenalumar
- Carl Zeiss Jena Fluoval
- Carl Zeiss Jena Laboval Fluorescence

Description of this part for the microscope.

filter holder of Carl Zeiss Jena Vertival microscopes.

Description of this part for the microscope.

catalog number 451495-0100 (45 14 59 - 0100).

The basic versions of the Zeiss Axioskop 20 microscope, intended only for transmitted light operation, do not include any filter cube. In such cases, this type of blanking cover is required to prevent dust from entering the optics.

Description of this part for the microscope.

25).

This is a four-position fluorescence reflector / fluorescence slider / filter cube carrier used in fluorescence microscopy. The element contains four positions for fluorescence filter cubes or fluorescence filter sets.

The offered element contains the following fluorescence filter cubes:

I.
- Excitation: G365
- Beam splitter: FT395
- Barrier filter: LP420

This filter set is typically used for UV fluorescence applications including:
- DAPI
- Hoechst
- AMCA
- DyLight 350
- other UV excited fluorophores

II.
- Excitation: BP450-490
- Beam splitter: FT510
- Barrier filter: LP515

This filter set is typically used for:
- FITC
- GFP
- Fluorescein
- Alexa Fluor 488
- green fluorescence dyes

III.
- empty slot without filter cube

The free slot allows:
- installation of an additional filter set
- operation without fluorescence filters
- faster switching between fluorescence techniques

IV.
- Excitation: BP546
- Beam splitter: FT580
- Barrier filter: LP590

This filter set is typically used for:
- Rhodamine
- TRITC
- Cy3
- Texas Red
- red fluorescence dyes

Such fluorescence filters are commonly used in:
- cell biology
- histology
- cytology
- immunology
- microbiology
- laboratory diagnostics
- molecular biology
- DNA and RNA analysis
- fluorochrome analysis
- observation of fluorescently labelled antibodies
- tissue autofluorescence studies
- observation of chlorophyll and plant specimens
- analysis of FITC, DAPI, AMCA, Rhodamine and related fluorescent dyes
- fluorescence photomicrography
- material science and technical analysis
- observation of UV fluorescent minerals and substances

Such filter sets are also popular among hobbyists and fluorescence microscopy enthusiasts:
- observing insect autofluorescence
- observing UV fluorescent minerals
- fluorescence photography
- observing fungi, pollen and biological structures
- experiments with fluorescent dyes

This fluorescence reflector is compatible among others with the following Carl Zeiss microscopes:
- Carl Zeiss Axioplan
- Carl Zeiss Axioskop
- Carl Zeiss Axiophot
- Carl Zeiss Axiovert
- other Carl Zeiss fluorescence microscopes using the 4FL system

Different names are commonly used for this element, including:
- fluorescence reflector
- fluorescence slider
- fluorescence module
- filter cube carrier
- filter cube holder
- fluorescence turret
- fluorescence filter holder
- fluorescence filter cube
- fluorescence cassette
- fluorescence insert
- fluorescence block

Description of this part for the microscope.

parameters:
Filter colour: blue, matt
Function: NCB – converts the yellow light of the halogen lamp into naturally white light
Mounting diameter: adjustable from 25.5 mm to 26 mm

Mechanically, this filter is mounted on the front end of the halogen illuminator (over the light output opening). Its effect is to produce light with a colour similar to that emitted by a cool white LED.

Description of this part for the microscope.

inside the microscope base. It includes a mechanism that allows these filters to be inserted into or removed from the light path using control buttons. Since more than one filter can be placed in the light path at the same time, the mechanism also includes an additional (seventh) button that removes all filters from the light path simultaneously. Because of this seventh button, some descriptions incorrectly state that the microscope base contains seven filters.

The following filters are installed in this filter cassette:
- NCB filter (blue conversion filter that transforms yellow incandescent lamp light into naturally white light), Zeiss P/N 46 78 50 (yellow stripe on the push button)
- VG 9 green filter, Zeiss P/N 46 78 05 (green stripe on the push button)
- two neutral density filters 0.5, Zeiss P/N 46 78 40 (light grey stripes on the push button)
- neutral density filter 0.12, Zeiss P/N 46 78 41 (grey stripe on the push button)
- neutral density filter 0.03, Zeiss P/N 46 78 42 (dark grey stripe on the push button)

Combinations of the neutral density filters provide stepwise light transmission values ranging from 1.5% to 100%.

Each of these filters can be removed from its holder, resulting in a standard 32 mm round microscope filter that can also be used in microscopes made by other manufacturers. In addition, any other 32 mm round filter can be installed in this filter cassette, allowing the filter set of the microscope to be configured freely.

This filter cassette with its complete control mechanism fits all versions of Carl Zeiss Photomicroscope microscopes (also known as Zeiss PM or Zeiss Phomi) as well as Zeiss Universal microscopes. However, there are also some less common variants of the Zeiss Universal that do not include this filter mechanism inside the microscope base. Importantly, the Carl Zeiss Ultraphot microscope does not use this component.

The last photographs in the gallery indicate with an arrow exactly which component is being offered and where it is located inside the microscope. This allows unambiguous identification of the part.

Description of this part for the microscope.

reflector, performs two functions:
- it contains a barrier filter (emission filter) for the 450 nm wavelength
- it acts as an angular mirror, reflecting the light beam from the EPI fluorescence illuminator vertically downward through the objective toward the specimen.

This fluorescence filter cube fits the following EPI fluorescence microscopes:
- Carl Zeiss Jena Fluoval Fluoreszenz
- Carl Zeiss Jena Fluoval-2 (built on the Carl Zeiss Jena Amplival microscope platform)

Important – this microscope component does not fit the first version of the Carl Zeiss Jena Fluoval microscope. The Fluoval microscope (without "2") does not use a filter cube. Instead, it has a simple permanently mounted swing-out barrier filter.

The filter cube has two positions:
- empty, for normal transmitted-light operation
- 450 nm for reflected-light fluorescence (EPI) operation

Mechanically, this filter cube is installed between the objective nosepiece and the microscope head. It slides into a dedicated slot and moves along a dovetail guide. On the Carl Zeiss Jena Laboval Fluoreszenz microscope, this slot is located in the additional upper horizontal arm of the EPI fluorescence illuminator. On the Carl Zeiss Jena Fluoval-2 microscope, the slot is located in the EPI fluorescence arm, which is an integral part of the microscope. The Fluoval-2 is based on the Carl Zeiss Jena Amplival microscope, which has a modular design. Mechanically, the Fluoval-2 is essentially an Amplival equipped with an upper EPI fluorescence arm.

Sliding the cube along this dovetail guide switches between the empty position (transmitted light) and EPI fluorescence with the 450 nm filter.

Description of this part for the microscope.

This filter is mounted in the sheet-metal housing of the large HBO 250W fluorescence illuminator. This illuminator is part of a large mirror light beam splitter used in Carl Zeiss Jena Jenalumar microscopes. The KG filter is designed to absorb heat (infrared radiation) emitted by the high-pressure HBO mercury lamp.

Please note that there is also a version of the Carl Zeiss Jena Jenalumar microscopes with a small 50W HBO illuminator. This KG filter glass was removed from a large 250W illuminator.

On the used market, in microscopy forums and in microscopy groups, several names are used for this filter, all referring to the same type of filter:
- KG filter / KG glass
- heat filter
- heat absorbing filter
- heat absorption filter
- heat-absorbing glass
- heat absorption glass
- absorption filter
- IR filter
- infrared blocking filter
- infrared cut filter
- KG3 heat absorption filter

This microscope element is highly susceptible to damage. When this happens, the filter needs to be replaced, for example with the filter presented here. Here are examples of damage that can occur during use of the fluorescence illuminator and reasons why replacement of the filter may be necessary:
- the KG filter may crack or become cloudy due to overheating
- the filter may also chip or break as a result of mechanical damage, for example during transport
- the filter may sometimes be damaged during improper maintenance

High-performance heat filters such as this KG filter used with a 250W lamp are very often used by people building their own DIY projects. The housing with screw mounting makes this particularly convenient.

The last photo in the gallery shows the housing of the HBO 250W illuminator (lampenhaus), with an arrow indicating which element is being described and where it is located inside the illuminator. This makes it possible to identify the element unambiguously.