Home page of the microscope service parts, repair and spare parts online catalog - Sermik-Oplatek Mikroskopservice Dresden f.n.n.Z

Description of this part for the microscope.

rare on the used microscope market. It can be used with microscopes such as:
- Carl Zeiss Jena Jenamed Fluorescence (Fluoreszenz)
- Carl Zeiss Jena Jenalumar
- Carl Zeiss Jena Fluoval
- Carl Zeiss Jena Laboval Fluorescence

Description of this part for the microscope.

filter is exceptionally rare on the used microscope market. It can be used with microscopes such as:
- Carl Zeiss Jena Jenamed Fluorescence (Fluoreszenz)
- Carl Zeiss Jena Jenalumar
- Carl Zeiss Jena Fluoval
- Carl Zeiss Jena Laboval Fluorescence

Description of this part for the microscope.

Jenalumar 50

Please note the following:
- both microscopes listed here use this illuminator for EPI fluorescence, i.e. reflected light. This is not transmitted-light fluorescence.
- this illuminator requires a Carl Zeiss Jena SH 50 power supply for operation
- the Carl Zeiss Jena Jenalumar microscope was produced in two variants - this illuminator fits the Jenalumar version with two small, separate illumination modules
- information can be found on the Internet claiming that this illuminator is suitable for Carl Zeiss Jena Jenavert microscopes. This is NOT correct.
- an HBO lamp is not the same as an XBO lamp.
- Jenamed microscopes other than the "Fluorescence" version have a differently mounted head and this illuminator CANNOT be installed on them.

Important - operating used, overheated HBO lamps, lamps with unknown operating time or from an uncertain source may result in explosion, damage to the illuminator, contamination of the room and a risk to the user's life and health. For this reason, this illuminator DOES NOT INCLUDE a light source (i.e. it does not include the HBO mercury burner).

Description of this part for the microscope.

rare on the used microscope market. It can be used with microscopes such as:
- Carl Zeiss Jena Jenamed Fluorescence (Fluoreszenz)
- Carl Zeiss Jena Jenalumar
- Carl Zeiss Jena Fluoval
- Carl Zeiss Jena Laboval Fluorescence

Description of this part for the microscope.

rare on the used microscope market. It can be used with microscopes such as:
- Carl Zeiss Jena Jenamed Fluorescence (Fluoreszenz)
- Carl Zeiss Jena Jenalumar
- Carl Zeiss Jena Fluoval
- Carl Zeiss Jena Laboval Fluorescence

Description of this part for the microscope.

rare on the used microscope market. It can be used with microscopes such as:
- Carl Zeiss Jena Jenamed Fluorescence (Fluoreszenz)
- Carl Zeiss Jena Jenalumar
- Carl Zeiss Jena Fluoval
- Carl Zeiss Jena Laboval Fluorescence

Description of this part for the microscope.

17-A
Magnification: 50x
Numerical aperture N.A.: 0.95
Optical correction: Apochromat
Additionally: cover glass thickness correction collar, range 0.12 - 0.22 mm
Tube length: infinity
Thread: M25
Cover glass: yes, required
Immersion: no (so-called dry objective)

Commonly used, cheap cover glasses are 0.17 mm thick mostly on paper. Even the standard for class #1.5 allows a range of 0.16-0.19 mm, and cheap Chinese cover glasses in practice come in anywhere from approx. 0.13 to 0.25 mm, often not matching the thickness stated on the box. Only #1.5H (high precision) cover glasses have a guaranteed thickness of 0.170 mm with a tolerance of +/- 0.005 mm. With high-aperture dry objectives such as this Apochromat 50x/0.95, variation in cover glass thickness noticeably reduces image contrast and resolution (spherical aberration). The correction collar fitted to this objective lets you adjust the optics to the combined thickness of the cover glass and the mounting medium layer beneath it, within a range of 0.12 mm to 0.22 mm. This compensates for most of the variation encountered in practice.

This objective fits, among others, the following microscopes:
- Carl Zeiss Jena Jenamed
- Carl Zeiss Jena Jenalab
- Carl Zeiss Jena Jenaval
- Carl Zeiss Jena Jenalumar

As for the Carl Zeiss Jena Jenamed microscope, this objective fits every variant:
- Carl Zeiss Jena Jenamed variant
- Carl Zeiss Jena Jenamed Fluorescence
- Carl Zeiss Jena Jenamed hematology
- Carl Zeiss Jena Jenamed cytology
- Carl Zeiss Jena Jenamed 2

Description of this part for the microscope.

Carl Zeiss Jena
Model: KP425
Type: interference excitation filter for fluorescence
Diameter: 32 mm
Mirror coating: yes

Filters 32 mm in diameter can be used in most microscopes from many manufacturers. In Carl Zeiss Jena microscopes they can be used, among others, in the following fluorescence microscopes:
- Carl Zeiss Jena Jenamed Fluorescence (Fluoreszenz)
- Carl Zeiss Jena Jenalumar
- Carl Zeiss Jena Fluoval
- Carl Zeiss Jena Laboval Fluorescence

Note - because this is an interference filter (it has a mirrored surface), the angle at which the filter sits relative to the incoming light matters and affects its parameters.

The coating on interference filters is sensitive not only to mechanical damage, for example scratches from improper storage or cleaning. Several decades of work right next to the HBO lamp can cloud it or make it delaminate, which shows as patches or rainbow islands that do not disappear when you rotate it. In that case the best solution is to replace the filter with one like the one I offer here.

Description of this part for the microscope.

Carl Zeiss Jena
Model: KP490
Type: interference excitation filter for fluorescence
Diameter: 32 mm
Mirror coating: yes

Filters 32 mm in diameter can be used in most microscopes from many manufacturers. In Carl Zeiss Jena microscopes they can be used, among others, in the following fluorescence microscopes:
- Carl Zeiss Jena Jenamed Fluorescence (Fluoreszenz)
- Carl Zeiss Jena Jenalumar
- Carl Zeiss Jena Fluoval
- Carl Zeiss Jena Laboval Fluorescence

Note - this is an interference filter, which you can tell from the mirrored surface. For that reason the angle at which the filter sits relative to the incoming light matters and affects its parameters.

The coating on interference filters is sensitive to mechanical damage, for example scratches from improper storage or cleaning. Age is a separate matter - several decades of work right next to the HBO lamp can cloud it or make it delaminate, which shows as patches or rainbow islands that do not disappear when you rotate it. In that case the best solution is to replace the filter with one like the one I offer here.

Description of this part for the microscope.

Carl Zeiss Jena
Model: KP560
Type: interference excitation filter for fluorescence
Diameter: 32 mm
Mirror coating: yes

Filters 32 mm in diameter can be used in most microscopes from many manufacturers. In Carl Zeiss Jena microscopes they can be used, among others, in the following fluorescence microscopes:
- Carl Zeiss Jena Jenamed Fluorescence (Fluoreszenz)
- Carl Zeiss Jena Jenalumar
- Carl Zeiss Jena Fluoval
- Carl Zeiss Jena Laboval Fluorescence

Note - the mirrored surface gives away that this is an interference filter. With filters of this kind the angle to the incoming light is not indifferent and affects their parameters.

The coating on interference filters is sensitive to mechanical damage, for example scratches from improper storage or cleaning. The second enemy is time - several decades of work right next to the HBO lamp can cloud it or make it delaminate, which shows as patches or rainbow islands that do not disappear when you rotate it. In that case the best solution is to replace the filter with one like the one I offer here.

Description of this part for the microscope.

Carl Zeiss Jena
Model: B226g
Filter type: red-absorbing glass filter (the manufacturer calls it Rotsperrfilter)
Diameter: 32 mm
Colour: blue
Toughened version: yes

The letter "g" at the end of the designation is short for the German gehärtet, meaning toughened - the manufacturer gave it to filters meant to work right next to high-power lamps, especially HBO mercury burners, where ordinary glass does not withstand the high temperature.

Filters 32 mm in diameter can be used in most microscopes from many manufacturers. In Carl Zeiss Jena microscopes they can be used, among others, in the following fluorescence microscopes:
- Carl Zeiss Jena Jenamed Fluorescence (Fluoreszenz)
- Carl Zeiss Jena Jenalumar
- Carl Zeiss Jena Fluoval / Fluoval-2
- Carl Zeiss Jena Laboval Fluorescence

In the excitation path this filter does not work on its own. It is added to the main filter to cut off the red passed by that filter and to narrow the excitation band further.

Description of this part for the microscope.

Carl Zeiss Jena
Model: B228g
Filter type: red-absorbing glass filter (the manufacturer calls it Rotsperrfilter)
Diameter: 32 mm
Colour: blue
Toughened version: yes

The "g" at the end of the designation stands for the German gehärtet, meaning toughened. The manufacturer gave it to filters meant to work right next to high-power lamps, especially HBO mercury burners, where ordinary glass does not withstand the high temperature.

Filters 32 mm in diameter can be used in most microscopes from many manufacturers. In Carl Zeiss Jena microscopes they can be used, among others, in the following fluorescence microscopes:
- Carl Zeiss Jena Jenamed Fluorescence (Fluoreszenz)
- Carl Zeiss Jena Jenalumar
- Carl Zeiss Jena Fluoval / Fluoval-2
- Carl Zeiss Jena Laboval Fluorescence

In the excitation path this filter does not work on its own. It is added to the main filter to cut off the red that filter passes and to narrow the excitation band further.

Description of this part for the microscope.

Carl Zeiss Jena
Model: B229g
Filter type: red-absorbing glass filter (the manufacturer calls it Rotsperrfilter)
Diameter: 32 mm
Colour: blue
Toughened version: yes

The "g" at the end of the designation stands for the German gehärtet, meaning toughened. The manufacturer gave it to filters meant to work right next to high-power lamps, especially HBO mercury burners, where ordinary glass does not withstand the high temperature.

Filters 32 mm in diameter can be used in most microscopes from many manufacturers. In Carl Zeiss Jena microscopes they can be used, among others, in the following fluorescence microscopes:
- Carl Zeiss Jena Jenamed Fluorescence (Fluoreszenz)
- Carl Zeiss Jena Jenalumar
- Carl Zeiss Jena Fluoval / Fluoval-2
- Carl Zeiss Jena Laboval Fluorescence

In the excitation path this filter does not work on its own. It is added to the main filter to cut off the red that filter passes and to narrow the excitation band further.

Description of this part for the microscope.

filter:
Manufacturer: Carl Zeiss Jena
Model: U204g
Filter type: glass excitation filter for UV fluorescence (the manufacturer calls it Erregerfilter)
Glass type: UG 1
Diameter: 32 mm
Thickness: 2 mm
Colour: black-violet, passes very little visible light
Toughened version: yes

The g at the end of the designation stands for the German gehärtet, meaning toughened. The manufacturer gave it to filters meant to work right next to high-power lamps, especially HBO mercury burners, where ordinary glass does not withstand the high temperature. With a UV excitation filter this matters especially, because it sits at the hottest point of the whole path and absorbs most of the burner energy.

Filters 32 mm in diameter can be used in most microscopes from many manufacturers. In Carl Zeiss Jena microscopes they can be used, among others, in the following fluorescence microscopes:
- Carl Zeiss Jena Jenamed Fluorescence (Fluoreszenz)
- Carl Zeiss Jena Jenalumar
- Carl Zeiss Jena Fluoval / Fluoval-2
- Carl Zeiss Jena Laboval Fluorescence

The manufacturer catalogue gives the following transmission values for this glass at 2 mm thickness:
- 0.015 at 302 nm
- 0.093 at 312 nm
- 0.435 at 334 nm
- 0.644 at 366 nm

Across the whole visible range, from 405 to 644 nm, transmission is zero. This means the filter works almost entirely on the 366 nm mercury line, exactly where the HBO burner is strongest in the ultraviolet.

One property of this glass is worth knowing: above 700 nm it starts to transmit again, and at 750 nm transmission reaches 0.210. This infrared leak is invisible to the eye, but it heats the specimen and spoils contrast in photographs.

In the excitation path U204 is also combined with a red-absorbing filter. The manufacturer documentation shows such a pair with the B226 filter, which cleans up the excitation band further.

The same UG 1 glass was supplied in two thicknesses, each with its own designation:
- The Carl Zeiss Jena U204 filter is UG 1 glass 2 mm thick
- The Carl Zeiss Jena U205 filter is UG 1 glass 4 mm thick

The thicker U205 cuts more sharply, but passes about a third less energy on the 366 nm line, so the image is noticeably darker.

A safety note: this filter passes ultraviolet, which is invisible and harmful to the eyes. When working with it, never look into the illumination path without a barrier filter in place.