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Description of this part for the microscope.

yes, 0.17 mm
Thread: RMS
Tube length: 160 mm
Focal length: 45 mm
Immersion: yes, oil immersion
Thread: RMS

Only towards the end of its operations did Hertel & Reuss begin producing microscopes with objectives having a 45 mm parfocal length. These were the Hertel & Reuss Labopan and Superpan models. Previously, microscopes from this company used objectives with a 37 mm parfocal length.

This objective fits practically any microscope from any manufacturer, provided that it has an RMS thread and supports objectives with TL=160 mm. Several examples of such microscopes are listed in the "Compatibility" section, but this is not a complete list.

Description of this part for the microscope.

10
Cover glass: not required
Thread: RMS
Tube length: 160 mm
Focal length: 45 mm
Immersion: no (dry objective)
Thread: RMS

This objective can be used both:
- with biological microscopes using transmitted-light illumination
- with inverted microscopes
- with microscopes with reflected-light illumination
- for macro photography by directly connecting it to a camera

This objective fits practically any microscope from any manufacturer, provided that it has an RMS thread and supports objectives for TL=160 mm. Several examples of such microscopes are listed in the "compatibility" section, but this is not a complete list.

Several facts are worth emphasizing:
- many Hund Wetzlar microscope models are identical to models sold by Will Wetzlar due to their joint production. For example, the BX300 and Wilovert models even have the same name. Therefore, Hund Wetzlar microscope objectives can also be used with Will Wetzlar microscopes.
- the companies Eschenbach, Kremp Wetzlar, CBS Beck Kassel and Kosmos Stuttgart also used Will Wetzlar objectives, so Hund Wetzlar microscope objectives can also be used with microscopes from these manufacturers - but it is worth checking the parfocal length first (37 mm or 45 mm)
- objectives with magnifications below 16x (e.g. 4x / 5x or 10x) fit both classic biological microscopes, microscopes with reflected-light illuminators and inverted microscopes.

Description of this part for the microscope.

25
Cover glass: not required
Thread: RMS
Tube length: 160 mm
Focal length: 45 mm
Immersion: no (dry objective)
Thread: RMS

This objective can be used both:
- with biological microscopes using transmitted-light illumination
- with inverted microscopes
- with microscopes with reflected-light illumination
- for macro photography by directly connecting it to a camera

This objective fits practically any microscope from any manufacturer, provided that it has an RMS thread and supports objectives for TL=160 mm. Several examples of such microscopes are listed in the "compatibility" section, but this is not a complete list.

Several facts are worth emphasizing:
- many Hund Wetzlar microscope models are identical to models sold by Will Wetzlar due to their joint production. For example, the BX300 and Wilovert models even have the same name. Therefore, Hund Wetzlar microscope objectives can also be used with Will Wetzlar microscopes.
- the companies Eschenbach, Kremp Wetzlar, CBS Beck Kassel and Kosmos Stuttgart also used Will Wetzlar objectives, so Hund Wetzlar microscope objectives can also be used with microscopes from these manufacturers - but it is worth checking the parfocal length first (37 mm or 45 mm)
- objectives with magnifications below 16x (e.g. 4x / 5x or 10x) fit both classic biological microscopes, microscopes with reflected-light illuminators and inverted microscopes.

Description of this part for the microscope.

65
Cover glass: yes, 0,17 mm
Thread: RMS
Tube length: 160 mm
Focal length: 45 mm
Immersion: no (dry objective)
Thread: RMS

This objective fits practically any microscope from any manufacturer, provided that it has an RMS thread and supports objectives for TL=160 mm. Several examples of such microscopes are listed in the "compatibility" section, but this is not a complete list.

- many Hund Wetzlar microscope models are identical to models sold by Will Wetzlar due to their joint production. For example, the BX300 and Wilovert models even have the same name. Therefore, Hund Wetzlar microscope objectives can also be used with Will Wetzlar microscopes.
- the companies Eschenbach, Kremp Wetzlar, CBS Beck Kassel and Kosmos Stuttgart also used Will Wetzlar objectives, so Hund Wetzlar microscope objectives can also be used with microscopes from these manufacturers - but it is worth checking the parfocal length first (37 mm or 45 mm)

Description of this part for the microscope.

25
Cover glass: yes, 0.17 mm
Thread: RMS
Tube length: 160 mm
Focal length: 45 mm
Immersion: yes, oil immersion
Thread: RMS

This objective fits practically any microscope from any manufacturer, provided that it has an RMS thread and supports objectives for TL=160 mm. Several examples of such microscopes are listed in the "compatibility" section, but this is not a complete list.

- many Hund Wetzlar microscope models are identical to models sold by Will Wetzlar due to their joint production. For example, the BX300 and Wilovert models even have the same name. Therefore, Hund Wetzlar microscope objectives can also be used with Will Wetzlar microscopes.
- the companies Eschenbach, Kremp Wetzlar, CBS Beck Kassel and Kosmos Stuttgart also used Will Wetzlar objectives, so Hund Wetzlar microscope objectives can also be used with microscopes from these manufacturers - but it is worth checking the parfocal length first (37 mm or 45 mm)

Description of this part for the microscope.

for inverted and upright biological microscopes, made in Japan.

Basic objective parameters:
Manufacturer: Nikon, Japan
Model: Ph3 40 DL 0.55 LWD 160/0-2
Magnification: 40x
Numerical aperture (N.A.): 0.55
Optical correction: achromat
Phase contrast: Ph3 DL - Ph3 indicates the size of the phase ring (requires a matching Ph3 annulus in the condenser), DL (Dark Low) means dark, i.e. positive phase contrast - the object appears darker than the background
Working distance (WD): ~2.1 - 2.5 mm - LWD (Long Working Distance)
Correction collar: compensates for cover glass or vessel bottom thickness in the range 0 - 2.0 mm
Cover glass: the objective can be used without a cover glass, with a standard cover glass approx. 0.17 mm thick, and also with specimens observed through the bottom of a Petri dish up to 2.0 mm thick
Immersion: no (dry objective)
Tube length: 160 mm
Parfocal length: 45 mm
Thread: RMS
Optics: CF (Nikon CF-160) - requires no compensating eyepiece and allows photography by direct projection

This objective is suitable for:
- inverted microscopes - observation through the bottom of a Petri dish or cell culture flask
- upright biological microscopes - observation of specimens with or without a cover glass

Phase contrast work requires a compatible phase contrast condenser or a slider with a phase contrast annulus.

Commonly used, inexpensive cover glasses are 0.17 mm thick mainly in theory. Even the standard for class #1.5 allows a range of 0.16 - 0.19 mm, and in cheap Chinese cover glasses you will find in practice pieces from approx. 0.13 to 0.25 mm, often not matching the thickness stated on the box. Only #1.5H (high precision) cover glasses have a guaranteed thickness of 0.170 mm with a tolerance of +/- 0.005 mm. Variation in cover glass thickness clearly degrades image contrast and resolution (spherical aberration). The correction collar fitted to the objective offered here lets you adjust the optics to the combined thickness of the cover glass and the medium layer beneath it. This compensates for most of the variation found in practice.

In addition, the correction collar of this objective can be set to "0" for work without a cover glass, or to large values (up to 2.0 mm) to adapt the optics to observation through a microscope slide (0.8 - 1.2 mm), a glass Petri dish (usually 1.5 - 2.0 mm - thicker dishes are outside the collar range) or a plastic Petri dish (usually approx. 1 mm, in the range 0.8 - 1.2 mm).

This objective fits practically any microscope from any manufacturer, provided it has an RMS thread and uses objectives for 160 mm tube length with a parfocal length of 33, 37 or 45 mm. Some example microscopes are listed in the "compatibility" section, but the list is not complete.

Description of this part for the microscope.

objective:
Manufacturer: Zeiss West Germany
Engraving on the objective: Planapo 63/1,4 Oel 160/-
Manufacturer catalogue number: 46 18 40 - 9901 (also written as 461840-9901)
Magnification: 63x
Numerical aperture (N.A.): 1.4
Optical correction: plan-apochromatic
Immersion: yes, oil
Coverslip: according to the engraving on the barrel of this objective, it can be used both without a coverslip and with a standard coverslip of 0.17 mm thickness
Tube length: 160 mm
Parfocal distance: 45 mm
Thread: RMS
Specimen protection: yes, the objective has a spring-loaded mechanism preventing the slide with the specimen from being broken
Phase contrast: no - the objective has no phase rings inside
DIC: prisms allowing work in DIC mode are available on the market for this objective (sold separately)

This objective fits practically any microscope of any manufacturer, provided it has an RMS thread and accepts objectives for a 160 mm tube length with a parfocal distance of 33, 37 or 45 mm. A few such microscopes are listed as examples in the "compatibility" section, but this is not a complete list.