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Description of this part for the microscope.

Description of this part for the microscope.

inside which the power cables to the lamp are routed. These cables are covered inside the arm by a metal cover. This is exactly the element (metal cover) shown and offered here.

Description of this part for the microscope.

for inverted and upright biological microscopes, made in Japan.

Basic objective parameters:
Manufacturer: Nikon, Japan
Model: Ph3 40 DL 0.55 LWD 160/0-2
Magnification: 40x
Numerical aperture (N.A.): 0.55
Optical correction: achromat
Phase contrast: Ph3 DL - Ph3 indicates the size of the phase ring (requires a matching Ph3 annulus in the condenser), DL (Dark Low) means dark, i.e. positive phase contrast - the object appears darker than the background
Working distance (WD): ~2.1 - 2.5 mm - LWD (Long Working Distance)
Correction collar: compensates for cover glass or vessel bottom thickness in the range 0 - 2.0 mm
Cover glass: the objective can be used without a cover glass, with a standard cover glass approx. 0.17 mm thick, and also with specimens observed through the bottom of a Petri dish up to 2.0 mm thick
Immersion: no (dry objective)
Tube length: 160 mm
Parfocal length: 45 mm
Thread: RMS
Optics: CF (Nikon CF-160) - requires no compensating eyepiece and allows photography by direct projection

This objective is suitable for:
- inverted microscopes - observation through the bottom of a Petri dish or cell culture flask
- upright biological microscopes - observation of specimens with or without a cover glass

Phase contrast work requires a compatible phase contrast condenser or a slider with a phase contrast annulus.

Commonly used, inexpensive cover glasses are 0.17 mm thick mainly in theory. Even the standard for class #1.5 allows a range of 0.16 - 0.19 mm, and in cheap Chinese cover glasses you will find in practice pieces from approx. 0.13 to 0.25 mm, often not matching the thickness stated on the box. Only #1.5H (high precision) cover glasses have a guaranteed thickness of 0.170 mm with a tolerance of +/- 0.005 mm. Variation in cover glass thickness clearly degrades image contrast and resolution (spherical aberration). The correction collar fitted to the objective offered here lets you adjust the optics to the combined thickness of the cover glass and the medium layer beneath it. This compensates for most of the variation found in practice.

In addition, the correction collar of this objective can be set to "0" for work without a cover glass, or to large values (up to 2.0 mm) to adapt the optics to observation through a microscope slide (0.8 - 1.2 mm), a glass Petri dish (usually 1.5 - 2.0 mm - thicker dishes are outside the collar range) or a plastic Petri dish (usually approx. 1 mm, in the range 0.8 - 1.2 mm).

This objective fits practically any microscope from any manufacturer, provided it has an RMS thread and uses objectives for 160 mm tube length with a parfocal length of 33, 37 or 45 mm. Some example microscopes are listed in the "compatibility" section, but the list is not complete.

Description of this part for the microscope.

5x
Magnification: 2.5x
Mounting diameter: 23.2 mm

Please note: this is a "CF" projection photo eyepiece, meaning "Chrome Free" (chromatic aberration free). The eyepiece itself carries no chromatic correction - for correct results it has to sit in the microscope objective. In the case of Nikon, those are the CF (CFN) and CFI objectives.

The standard 23.2 mm mounting diameter allows it to be used in the projection tubes of many microscopes from various manufacturers - both biological and stereo ones.

It has to be said that this is a very universal projection eyepiece. In the "compatibility" section I list only a small part of the microscopes this photo eyepiece can be used with.

From the experience of people I know, only testing a projection eyepiece yourself on a specific microscope setup gives you certainty about how it will behave.

With every configuration - different objectives, a different tube length, a different photo adapter, camera, DSLR, sensor size - the user will get a different result.

This applies to tests both on microscopes with finite tube length optics (for example the Nikon Labophot with 160 mm optics) and infinity optics (for example the Nikon Eclipse E600 infinity), and both on older stereo microscopes (for example the Nikon SMZ-10) and somewhat newer ones such as the Nikon SMZ-1500.

Description of this part for the microscope.

5
Magnification: 5x
Field number F.N.: 25
Mounting diameter: 23.2 mm

Please note: PLI is a series of projection eyepieces assigned to the CFI60 system (optics corrected for infinity / "I" as in "infinity"). I have, however, personally seen eyepieces from this series used on microscopes with optics corrected for a finite tube length (160 mm, to be precise).

This eyepiece contains no chromatic correction lenses, so that correction has to be built into the objective (in the case of Nikon objectives these are the CF / CFN / CFI series).

The standard 23.2 mm mounting diameter allows it to be used in the projection tubes of many microscopes from various manufacturers - both biological and stereo ones. It therefore has to be regarded as a very universal projection eyepiece. In the "compatibility" section I list only a small part of the microscopes this photo eyepiece can be used with.

From the experience of people I know, only testing a projection eyepiece yourself on a specific microscope setup gives you certainty about how it will behave.

With every configuration - different objectives, a different tube length, a different photo adapter, camera, DSLR, sensor size - the user will get a different result.

This applies to tests both on microscopes with finite tube length optics (for example the Nikon Alphaphot with 160 mm optics) and infinity optics (for example the Nikon Eclipse TS-100 infinity), and both on older stereo microscopes (for example the Nikon SMZ-2T) and somewhat newer ones such as the Nikon SMZ-18.