Home page of the microscope service parts, repair and spare parts online catalog - Sermik-Oplatek Mikroskopservice Dresden f.n.n.Z

Description of this part for the microscope.

65 160/0.17
Manufacturer part number: 46 07 10-9904 (alternative notation: 460710 - 9904)
Color correction: Planachromat
Numerical aperture N.A.: 0.65
Tube length: 160 mm
Focal length: 45 mm
Cover glass: yes, 0.17 mm thickness
Immersion: no (dry objective)
Thread: RMS

Description of this part for the microscope.

bright field
- dark field
- oblique illumination
- phase contrast

It can work with phase objectives of different magnifications and from different manufacturers (it is sufficient to manually adjust the ring diameter).

Basic parameters:
Manufacturer: Leitz Wetzlar
Model: Heine condenser
Mounting diameter: 39.5 mm
Numerical aperture N.A.: variable, up to 1.4

This Heine condenser was designed for microscopes with a condenser holder in the form of a circular clamp (e.g. Leitz SM microscopes). It is not a condenser that slides into mounting rails (as found, for example, in the Leitz Orthoplan microscope).

This condenser fits microscopes from many manufacturers (e.g. PZO, Steindorff Berlin, Zeiss, Carl Zeiss Jena, Leitz, Kremp Wetzlar, Will Wetzlar and many others) equipped with a 39.5 mm condenser holder.

Description of this part for the microscope.

Carl Zeiss Jena, PZO, Meopta, Olympus, IOR and others.

This tube makes it possible to connect a camera or photographic camera to a trinocular head or directly to the microscope body, even when no trinocular head is installed.

Originally, this adapter was designed for the Lomo TRN-1 trinocular head. This head was used in Lomo Polam polarizing microscopes from the early 1970s onwards.

Several remarks regarding the model designation of the trinocular head for which this component was originally intended:
- the name Lomo TRN-1 is the Latin alphabet designation. In Cyrillic script the model designation appears as TPH (for example model TPH1Y4.2), and this designation is used in the manual for the Lomo Polam L-211 microscope
- the vast majority of these trinocular heads are not marked with any model designation
- the Envirotools website http://www.envirotools.hu/ateso_fenyu_polarizacios_mikroszkop.htm refers to this trinocular head as HU-POL

In the last photograph, an arrow indicates exactly which component is being offered and where it is located in the Lomo Polam microscope, allowing this part to be identified unambiguously.

Basic technical parameters of this tube:
Mounting method to the trinocular head or microscope: 41 mm dovetail mount (this is also the Carl Zeiss Jena standard)
Camera mounting method: male M39 thread
Alternative camera mounting method: since the M39 thread can be removed, a plain smooth tube with a diameter of 35 mm remains
Additional optics: yes, the original optics are installed inside, although they can be removed, leaving only an "empty tube".

This is not "a tube dedicated to a specific microscope model". It is a tube originally mounted on the third port (photo port) of a trinocular head. Since a trinocular head can be installed on microscopes other than the model with which it was originally supplied, this tube can be regarded as a universal accessory.

These technical parameters make it possible to use this tube with virtually any microscope manufactured by:
- Lomo (for example Biolam, Polam, MBB-1A and others)
- Carl Zeiss Jena (for example Ergaval, Amplival, Jenamed and others)
- IOR, Meopta and other manufacturers using a 41 / 41.5 mm dovetail mount

In addition, a 41 mm dovetail tube can easily be mounted on microscopes equipped with a 42 mm or 43 mm opening, for example:
- Olympus (for example CH, CH-2, BH-2 models)
- PZO (for example Biolar, Studar, MB-30 and others)

The entire list of microscope manufacturers and models given above may also be treated as a list of microscopes compatible with the Lomo TRN-1 trinocular head. Indeed, many examples can be found on the Internet showing this head being used with microscopes made by manufacturers other than Lomo.

Because this tube is equipped with an M39 thread by default, almost any camera or photographic camera can be attached by means of a suitable adapter, for example:
- devices with M42 threads
- devices with C-mount threads
- Nikon, Sony, Olympus and other camera standards

This is therefore a universal photo tube featuring a 41 mm dovetail on one side and either an M39 thread or a 35 mm tube on the other side.

In the image gallery, every photograph includes a millimetre scale, and in addition, the dimensions of the dovetail, tube and thread have been measured with calipers so that compatibility can be determined precisely.

Description of this part for the microscope.

(8)
Magnification: 20x
Field of view: 8
Mounting diameter: 23.2 mm
Designation: PK = plan compensating - recommended for use with plan achromatic objectives, but they also work perfectly with achromatic and apochromatic objectives

Due to their very high quality, these eyepieces are often used together with microscopes from other manufacturers, especially when Carl Zeiss Jena Planachromat objectives are installed.

Important: these eyepieces can be used both with finite tube microscopes (e.g. 160 mm) and infinity-corrected microscopes. This means that they can be used, for example, both on a Carl Zeiss Jena Amplival microscope and on a Carl Zeiss Jena NU-2 microscope.

Note - in my opinion, eyepieces with such a high magnification are recommended rather for "inspection" than for continuous work. I deliberately write "I recommend" because this is my personal opinion based on practical use and not on the manufacturer's documentation.
An eyepiece magnification of 20x should rather be treated as a zoom without revealing additional details (the resolution is not increased). This is important, for example, in heads with built-in magnification (e.g. Carl Zeiss Jena heads with built-in magnification of 1.5x or 1.6x) - when working with a 100x oil immersion objective, we theoretically obtain 100*1.5*20 = 3000x, while physics does not allow the human eye to magnify visible light beyond approximately 1250x - therefore this is a so-called "empty magnification".

Description of this part for the microscope.

Zeiss Jena
Model: Apl 1.4
Holder name according to the manufacturer's catalogue: Kondensor-Einhänger mo
Condenser name according to the manufacturer's catalogue: Aplanatischer Kondensor 1.4 mo / ms
Version: grey version from a Carl Zeiss Jena Ergaval microscope (as opposed to the black version used, for example, on Carl Zeiss Jena Nf microscopes)
Numerical aperture N.A.: 1.4
Aperture diaphragm: yes, mounted in the condenser holder
Wide-field lens: yes, swing-out type
Removable top lens: yes, unscrewable
Mounting diameter: the optical section (the condenser without the iris diaphragm) has a mounting diameter of 39.5 mm and can be unscrewed from the condenser holder

The complete assembly consists of the following components:
- upper Apl 1.4 lens
- removable condenser (mo / ms)
- iris diaphragm attached to the condenser holder
- condenser holder mo
- swing-out wide-field lens
- two centring screws

The last photograph in the gallery shows a page from the manufacturer's original catalogue. Arrows indicate both this exact condenser holder model and the matching condenser.

Mechanically, this condenser is mounted using a dovetail guide located beneath the microscope stage. On the Carl Zeiss Jena Ergaval this is a separate condenser mounting assembly, while on the Carl Zeiss Jena Amplival the dovetail is integrated into the microscope stage support assembly.

Description of this part for the microscope.

unscrewed. It is mounted onto the dovetail guide located underneath the microscope stage carrier and secured with a clamping screw.

Carl Zeiss Jena Ergaval microscopes were originally equipped with a condenser attached to the "MO" holder. This holder has a permanently mounted iris diaphragm and wide-field lens, while the condenser itself is screwed into the upper thread. In this configuration, the condenser cannot be exchanged.
The Carl Zeiss Jena Fluoval uses a similar arrangement, whereas the Carl Zeiss Jena Amplival was originally equipped with a panchromatic condenser.

If the user wishes to use other condensers (including those made by manufacturers other than Carl Zeiss Jena), the original condenser holder must be replaced with the MZ condenser holder. Once this has been done, any microscope condenser with a mounting diameter of 39.5 mm can be installed. Condensers with a 37 mm mounting diameter can also be used if they are fitted with a suitable adapter ring.

This is exactly such a holder for interchangeable condensers.

Basic specifications:
Manufacturer: Carl Zeiss Jena
Original documentation name and model: Kondensor-Einhänger MZ
Colour: grey, the version manufactured for the Mikroval microscope series
Internal diameter: for mounting condensers with a 39.5 mm mounting diameter (for example the Carl Zeiss Jena cardioid darkfield condenser)
Condenser retaining screw: yes, central condenser clamping screw
Centering screws: yes, two condenser centering screws
Microscope mounting: dovetail

Although this component is grey, it is also compatible with older black Carl Zeiss Jena microscopes from the 1950s and 1960s (it simply will not look completely original unless it is repainted).

This condenser holder is compatible with, among others:
- Carl Zeiss Jena Ng (black microscope)
- Carl Zeiss Jena Polmi-A (black microscope)
- Carl Zeiss Jena Nf (black microscope)
- Carl Zeiss Jena Nu (the NU-2 version is grey)
- Carl Zeiss Jena Ergaval (grey microscope)
- Carl Zeiss Jena Fluoval / Fluoval-2 (grey microscope)
- Carl Zeiss Jena Amplival and its variants (grey microscope)
- Carl Zeiss Jena Docuval (grey microscope)

Various names are used for this microscope component, including:
- condenser holder
- condenser carrier
- condenser mount
- condenser support

The last photograph includes an excerpt from the original manufacturer's documentation. An arrow indicates this exact model of condenser holder, making it easy to identify the component.

Description of this part for the microscope.

unscrewed. It is mounted onto a dovetail guide located underneath the microscope stage carrier. The condenser holder is secured to the guide with a clamping screw.

It can accommodate any microscope condenser with a mounting diameter of 39.5 mm, regardless of the manufacturer. Condensers with a 37 mm mounting diameter can also be used if they are fitted with a suitable adapter ring.

Basic specifications:
Manufacturer: Carl Zeiss Jena
Original documentation name and model: Kondensor-Einhänger MZ
Colour: black, the version manufactured for the black Carl Zeiss Jena microscopes from the 1950s and 1960s
Internal diameter: for mounting condensers with a 39.5 mm mounting diameter (for example the Carl Zeiss Jena cardioid darkfield condenser)
Condenser retaining screw: yes, central condenser clamping screw
Centering screws: yes, two condenser centering screws
Microscope mounting: dovetail

Although this microscope component is black, it is also compatible with the newer grey Carl Zeiss Jena microscopes of the Mikroval series.

This condenser holder is compatible with, among others:
- Carl Zeiss Jena Ng (black microscope)
- Carl Zeiss Jena Polmi-A (black microscope)
- Carl Zeiss Jena Nf (black microscope)
- Carl Zeiss Jena Nu (the NU-2 version is grey)
- Carl Zeiss Jena Ergaval (grey microscope)
- Carl Zeiss Jena Fluoval / Fluoval-2 (grey microscope)
- Carl Zeiss Jena Amplival and its variants (grey microscope)
- Carl Zeiss Jena Docuval (grey microscope)

Various names are used for this microscope component, including:
- condenser holder
- condenser carrier
- condenser mount
- condenser support

The last photograph includes an excerpt from the original manufacturer's documentation. An arrow indicates this exact model of the condenser holder, making it easy to identify the component.

Description of this part for the microscope.

diameter: 23.2 mm

This eyepiece allows the microscope image to be projected onto a surface such as a wall, ceiling or projection screen.

Originally, this eyepiece model was supplied with the Carl Zeiss Jena Pictoval projection microscope. It can also be installed on virtually any microscope from any manufacturer with a 23.2 mm eyepiece tube.

Important: Projecting a microscope image requires a very large amount of light, so a high-power illuminator is necessary.

Description of this part for the microscope.

head in Carl Zeiss Jena Pictoval projection microscopes (microprojectors). The adjustable tilt angle of the eyepiece tube, which held a projection eyepiece (usually f=100), allowed the position of the projected image on the projection surface to be changed.

Basic parameters:
Manufacturer: Carl Zeiss Jena
Eyepiece mounting diameter: 23.2 mm
Mounting to the microscope arm: 41 mm diameter dovetail ring

Because this microscope head has a Carl Zeiss Jena standard microscope mount (a 41 mm diameter dovetail ring), it can be mounted on most microscopes from this manufacturer (e.g. Carl Zeiss Jena Eduval, Laboval, Ergaval, Amplival, etc.), as well as on microscopes made by Lomo (e.g. Lomo Biolam), IOR and Meopta.

It seems useful to mention a few points:
- never look through this microscope head with the naked eye if a projection eyepiece is installed in it and the head is mounted in a Carl Zeiss Jena Pictoval microprojector. This may result in loss of eyesight due to the very high light output emitted by this microscope
- when mounting this microscope head on a microscope other than a Carl Zeiss Jena Pictoval, a different magnification scale may be obtained than with the original heads designed for the particular microscope model
- projecting a microscope image requires a great deal of light, i.e. a powerful illuminator. This is worth remembering if you intend to use this head to project an image from biological microscopes with standard incandescent illumination (e.g. 15 W 6 V), which is definitely not sufficient
- visual observation using this microscope head may be inconvenient because it has a different (smaller) tilt angle than standard monocular or binocular microscope heads

Description of this part for the microscope.

yes, 0.17 mm
Thread: RMS
Tube length: 160 mm
Focal length: 45 mm
Immersion: yes, oil immersion
Thread: RMS

Only towards the end of its operations did Hertel & Reuss begin producing microscopes with objectives having a 45 mm parfocal length. These were the Hertel & Reuss Labopan and Superpan models. Previously, microscopes from this company used objectives with a 37 mm parfocal length.

This objective fits practically any microscope from any manufacturer, provided that it has an RMS thread and supports objectives with TL=160 mm. Several examples of such microscopes are listed in the "Compatibility" section, but this is not a complete list.

Description of this part for the microscope.

meters:
Manufacturer: Carl Zeiss Jena
Designation: Apl. 1,4
Name of the mount according to the manufacturer's catalogue: Kondensoreinhänger mfl 2, Bestellnummer 301080:021.26/0
Name of the condenser according to the manufacturer's catalogue: Aplanatischer Kondensor 1.4 mo / ms (applies only to the upper optical part)
Name of the condenser according to the manufacturer's catalogue: Kondensor hoher Lichtstärke mit Vorschaltsystem (applies to the entire module)
Version: grey colour with a black upper lens. Variant with a "dot" in the name after "Apl"
Numerical aperture N.A.: 1.4
Aperture diaphragm: yes, attached to the condenser mount
Wide-field lens: yes, it can be swung out of the optical path. This is the version with the large lens in a housing (see description below)
Removable upper lens: yes, it can be rotated
Mounting diameter: the optical part (condenser without diaphragm) has a mounting diameter of 39.5 mm and can be unscrewed from the condenser mount
Operating modes: the condenser can be used both as a dry condenser and as an immersion condenser

The entire part consists of the following elements:
- upper Apl. 1,4 lens
- screw-on/removable condenser (mo / ms)
- iris diaphragm screwed onto the condenser mount
- condenser mount model mfl 2
- swing-out wide-field lens
- two centering screws

This condenser is mainly used in Carl Zeiss Jena Fluoval / Fluoval-2 fluorescence microscopes. It is optimal for use with high-numerical-aperture apochromatic microscope objectives. For precisely this reason, users often install it on Carl Zeiss Jena Ergaval and Carl Zeiss Jena Amplival microscopes equipped with apochromatic objectives (Carl Zeiss Jena Apochromat).

It is worth mentioning again that this condenser can be used in two modes:
- without immersion oil on the upper lens of the condenser. In this case, it operates as a "dry condenser" and its numerical aperture N.A. is 0.9.
- with immersion oil on the upper lens of the condenser (i.e. underneath the microscope slide). In this case, it operates as an "immersion condenser" and its numerical aperture N.A. is 1.4, allowing a very bright image to be obtained - this is important in fluorescence microscopy or when using high-N.A. immersion objectives.

During production, this condenser was available in several versions:
- with a black upper lens, a dot in the model name (Apl. 1,4) and a wide-field module consisting of a single lens
- with a black upper lens, without a dot in the model name (Apl 1,4) and a single wide-field module consisting of several lenses (including one with a very small diameter)
- with a grey upper lens marked Apl 1,4mo2 and a single wide-field module consisting of several lenses (including one with a very small diameter)
- with a grey upper lens marked Apl 1,4mo2 and a wide-field module consisting of two independently swing-out lenses, each on its own mount

I have not found any information anywhere as to whether the above-mentioned versions differ in their operation. From experience, I know that they are used interchangeably - but I do not know whether any differences in operation can actually be noticed.

Mechanically, this condenser is mounted on dovetail rails located underneath the microscope stage. In Carl Zeiss Jena Ergaval microscopes, this is a separate condenser mounting block, whereas in Carl Zeiss Jena Fluoval / Amplival microscopes, the dovetail is located on the microscope stage holder block.

The manufacturer describes this condenser as follows: "High-intensity condenser with an auxiliary system for illuminating large fields while imaging the field diaphragm. [...] The condenser of the FLUOVAL 2 can be used as a dry and immersion condenser of the highest aperture to achieve the highest possible excitation intensity. It is equipped with a switchable wide-field system that illuminates the large fields of low-power objectives while providing a sharp image of the field diaphragm in the object plane."

Description of this part for the microscope.

meters:
Manufacturer: Carl Zeiss Jena
Designation: Apl. 1,4
Name of the mount according to the manufacturer's catalogue: Kondensoreinhänger mfl 2, Bestellnummer 301080:021.26/0
Name of the condenser according to the manufacturer's catalogue: Aplanatischer Kondensor 1.4 mo / ms (applies only to the upper optical part)
Name of the condenser according to the manufacturer's catalogue: Kondensor hoher Lichtstärke mit Vorschaltsystem (applies to the entire module)
Version: grey colour with a black upper lens. Variant without the "dot" in the name after "Apl"
Numerical aperture N.A.: 1.4
Aperture diaphragm: yes, attached to the condenser mount
Wide-field lens: yes, it can be swung out of the optical path. This is the version with the large lens in a housing (see description below)
Removable upper lens: yes, it can be rotated
Mounting diameter: the optical part (condenser without diaphragm) has a mounting diameter of 39.5 mm and can be unscrewed from the condenser mount
Operating modes: the condenser can be used both as a dry condenser and as an immersion condenser

The entire part consists of the following elements:
- upper Apl 1,4 lens
- screw-on/removable condenser (mo / ms)
- iris diaphragm screwed onto the condenser mount
- condenser mount model mfl 2
- swing-out wide-field lens
- two centering screws

This condenser is mainly used in Carl Zeiss Jena Fluoval / Fluoval-2 fluorescence microscopes. It is optimal for use with high-numerical-aperture apochromatic microscope objectives. For precisely this reason, users often install it on Carl Zeiss Jena Ergaval and Carl Zeiss Jena Amplival microscopes equipped with apochromatic objectives (Carl Zeiss Jena Apochromat).

It is worth mentioning again that this condenser can be used in two modes:
- without immersion oil on the upper lens of the condenser. In this case, it operates as a "dry condenser" and its numerical aperture N.A. is 0.9.
- with immersion oil on the upper lens of the condenser (i.e. underneath the microscope slide). In this case, it operates as an "immersion condenser" and its numerical aperture N.A. is 1.4, allowing a very bright image to be obtained - this is important in fluorescence microscopy or when using high-N.A. immersion objectives.

During production, this condenser was available in several versions:
- with a black upper lens, a dot in the model name (Apl. 1,4) and a wide-field module consisting of a single lens
- with a black upper lens, without a dot in the model name (Apl 1,4) and a single wide-field module consisting of several lenses (including one with a very small diameter)
- with a grey upper lens marked Apl 1,4mo2 and a single wide-field module consisting of several lenses (including one with a very small diameter)
- with a grey upper lens marked Apl 1,4mo2 and a wide-field module consisting of two independently swing-out lenses, each on its own mount

I have not found any information anywhere as to whether the above-mentioned versions differ in their operation. From experience, I know that they are used interchangeably - but I do not know whether any differences in operation can actually be noticed.

Mechanically, this condenser is mounted on dovetail rails located underneath the microscope stage. In Carl Zeiss Jena Ergaval microscopes, this is a separate condenser mounting block, whereas in Carl Zeiss Jena Fluoval / Amplival microscopes, the dovetail is located on the microscope stage holder block.

The manufacturer describes this condenser as follows: "High-intensity condenser with an auxiliary system for illuminating large fields while imaging the field diaphragm. [...] The condenser of the FLUOVAL 2 can be used as a dry and immersion condenser of the highest aperture to achieve the highest possible excitation intensity. It is equipped with a switchable wide-field system that illuminates the large fields of low-power objectives while providing a sharp image of the field diaphragm in the object plane."

Description of this part for the microscope.

10
Cover glass: not required
Thread: RMS
Tube length: 160 mm
Focal length: 45 mm
Immersion: no (dry objective)
Thread: RMS

This objective can be used both:
- with biological microscopes using transmitted-light illumination
- with inverted microscopes
- with microscopes with reflected-light illumination
- for macro photography by directly connecting it to a camera

This objective fits practically any microscope from any manufacturer, provided that it has an RMS thread and supports objectives for TL=160 mm. Several examples of such microscopes are listed in the "compatibility" section, but this is not a complete list.

Several facts are worth emphasizing:
- many Hund Wetzlar microscope models are identical to models sold by Will Wetzlar due to their joint production. For example, the BX300 and Wilovert models even have the same name. Therefore, Hund Wetzlar microscope objectives can also be used with Will Wetzlar microscopes.
- the companies Eschenbach, Kremp Wetzlar, CBS Beck Kassel and Kosmos Stuttgart also used Will Wetzlar objectives, so Hund Wetzlar microscope objectives can also be used with microscopes from these manufacturers - but it is worth checking the parfocal length first (37 mm or 45 mm)
- objectives with magnifications below 16x (e.g. 4x / 5x or 10x) fit both classic biological microscopes, microscopes with reflected-light illuminators and inverted microscopes.

Description of this part for the microscope.

25
Cover glass: not required
Thread: RMS
Tube length: 160 mm
Focal length: 45 mm
Immersion: no (dry objective)
Thread: RMS

This objective can be used both:
- with biological microscopes using transmitted-light illumination
- with inverted microscopes
- with microscopes with reflected-light illumination
- for macro photography by directly connecting it to a camera

This objective fits practically any microscope from any manufacturer, provided that it has an RMS thread and supports objectives for TL=160 mm. Several examples of such microscopes are listed in the "compatibility" section, but this is not a complete list.

Several facts are worth emphasizing:
- many Hund Wetzlar microscope models are identical to models sold by Will Wetzlar due to their joint production. For example, the BX300 and Wilovert models even have the same name. Therefore, Hund Wetzlar microscope objectives can also be used with Will Wetzlar microscopes.
- the companies Eschenbach, Kremp Wetzlar, CBS Beck Kassel and Kosmos Stuttgart also used Will Wetzlar objectives, so Hund Wetzlar microscope objectives can also be used with microscopes from these manufacturers - but it is worth checking the parfocal length first (37 mm or 45 mm)
- objectives with magnifications below 16x (e.g. 4x / 5x or 10x) fit both classic biological microscopes, microscopes with reflected-light illuminators and inverted microscopes.

Description of this part for the microscope.

65
Cover glass: yes, 0,17 mm
Thread: RMS
Tube length: 160 mm
Focal length: 45 mm
Immersion: no (dry objective)
Thread: RMS

This objective fits practically any microscope from any manufacturer, provided that it has an RMS thread and supports objectives for TL=160 mm. Several examples of such microscopes are listed in the "compatibility" section, but this is not a complete list.

- many Hund Wetzlar microscope models are identical to models sold by Will Wetzlar due to their joint production. For example, the BX300 and Wilovert models even have the same name. Therefore, Hund Wetzlar microscope objectives can also be used with Will Wetzlar microscopes.
- the companies Eschenbach, Kremp Wetzlar, CBS Beck Kassel and Kosmos Stuttgart also used Will Wetzlar objectives, so Hund Wetzlar microscope objectives can also be used with microscopes from these manufacturers - but it is worth checking the parfocal length first (37 mm or 45 mm)

Description of this part for the microscope.

25
Cover glass: yes, 0.17 mm
Thread: RMS
Tube length: 160 mm
Focal length: 45 mm
Immersion: yes, oil immersion
Thread: RMS

This objective fits practically any microscope from any manufacturer, provided that it has an RMS thread and supports objectives for TL=160 mm. Several examples of such microscopes are listed in the "compatibility" section, but this is not a complete list.

- many Hund Wetzlar microscope models are identical to models sold by Will Wetzlar due to their joint production. For example, the BX300 and Wilovert models even have the same name. Therefore, Hund Wetzlar microscope objectives can also be used with Will Wetzlar microscopes.
- the companies Eschenbach, Kremp Wetzlar, CBS Beck Kassel and Kosmos Stuttgart also used Will Wetzlar objectives, so Hund Wetzlar microscope objectives can also be used with microscopes from these manufacturers - but it is worth checking the parfocal length first (37 mm or 45 mm)

Description of this part for the microscope.

3x
Model: Semiplan 6.3/0.16 160/-
Serial number: 039 562 (039562)
Color correction: Semi-Planachromat
Numerical aperture: 0.16
Tube length: 160 mm
Focal length: 45 mm
Cover glass: no
Immersion: no
Thread: RMS

This is one of the most commonly used microscope objectives for macro photography and focus stacking.

Important - this objective IS SUITABLE for taking images using the "direct-projection" technique, as it does not require plan-compensating optics.

It can be used with any microscope from any manufacturer. Of course, the microscope must be compatible, meaning it supports RMS objectives, TL=160 mm, FL=45 mm.

Description of this part for the microscope.

for inverted and upright biological microscopes, made in Japan.

Basic objective parameters:
Manufacturer: Nikon, Japan
Model: Ph3 40 DL 0.55 LWD 160/0-2
Magnification: 40x
Numerical aperture (N.A.): 0.55
Optical correction: achromat
Phase contrast: Ph3 DL - Ph3 indicates the size of the phase ring (requires a matching Ph3 annulus in the condenser), DL (Dark Low) means dark, i.e. positive phase contrast - the object appears darker than the background
Working distance (WD): ~2.1 - 2.5 mm - LWD (Long Working Distance)
Correction collar: compensates for cover glass or vessel bottom thickness in the range 0 - 2.0 mm
Cover glass: the objective can be used without a cover glass, with a standard cover glass approx. 0.17 mm thick, and also with specimens observed through the bottom of a Petri dish up to 2.0 mm thick
Immersion: no (dry objective)
Tube length: 160 mm
Parfocal length: 45 mm
Thread: RMS
Optics: CF (Nikon CF-160) - requires no compensating eyepiece and allows photography by direct projection

This objective is suitable for:
- inverted microscopes - observation through the bottom of a Petri dish or cell culture flask
- upright biological microscopes - observation of specimens with or without a cover glass

Phase contrast work requires a compatible phase contrast condenser or a slider with a phase contrast annulus.

Commonly used, inexpensive cover glasses are 0.17 mm thick mainly in theory. Even the standard for class #1.5 allows a range of 0.16 - 0.19 mm, and in cheap Chinese cover glasses you will find in practice pieces from approx. 0.13 to 0.25 mm, often not matching the thickness stated on the box. Only #1.5H (high precision) cover glasses have a guaranteed thickness of 0.170 mm with a tolerance of +/- 0.005 mm. Variation in cover glass thickness clearly degrades image contrast and resolution (spherical aberration). The correction collar fitted to the objective offered here lets you adjust the optics to the combined thickness of the cover glass and the medium layer beneath it. This compensates for most of the variation found in practice.

In addition, the correction collar of this objective can be set to "0" for work without a cover glass, or to large values (up to 2.0 mm) to adapt the optics to observation through a microscope slide (0.8 - 1.2 mm), a glass Petri dish (usually 1.5 - 2.0 mm - thicker dishes are outside the collar range) or a plastic Petri dish (usually approx. 1 mm, in the range 0.8 - 1.2 mm).

This objective fits practically any microscope from any manufacturer, provided it has an RMS thread and uses objectives for 160 mm tube length with a parfocal length of 33, 37 or 45 mm. Some example microscopes are listed in the "compatibility" section, but the list is not complete.

Description of this part for the microscope.

of view number: 12
Mounting diameter: 23.2 mm
Designation: PK = plan compensating - recommended for work with planachromatic objectives, but they also work without any trouble with achromats or apochromats

Because of their very high quality they are often used on microscopes from other manufacturers as well, especially when Carl Zeiss Jena Planachromat objectives are mounted in them. The "compatibility" section lists a long series of microscopes these eyepieces fit - but that list is definitely not complete.

Important: these eyepieces work both on microscopes with a finite tube length (160 mm, for example) and on microscopes with infinity optics. That means you can use them on the Carl Zeiss Jena Amplival microscope just as well as on the Carl Zeiss Jena NU-2 microscope.

A note - eyepieces with such a strong magnification are in my opinion better suited to a quick look than to permanent work. I write "in my opinion" on purpose, because this comes from using them and not from the manufacturer's description.
A 16x eyepiece magnification should be treated as a zoom that brings out no additional detail (the resolution is not increased). This matters for example with heads that have their own extra magnification (such as Carl Zeiss Jena heads with a built-in 1.5x or 1.6x factor) - working with a 100x oil immersion objective you then theoretically get 100*1.5*16 = 2400x, while physics does not allow light from the visible range to be usefully magnified for the human eye beyond roughly 1250x - so this is what is called empty magnification.

Description of this part for the microscope.

5x
Field of view number: 16
Mounting diameter: 23.2 mm
Designation: PK = plan compensating - recommended for work with planachromatic objectives, but they also work without any trouble with achromats or apochromats

Because of their very high quality they are often used on microscopes from other manufacturers as well, especially when Carl Zeiss Jena Planachromat objectives are mounted in them. The "compatibility" section lists a long series of microscopes these eyepieces fit - but that list is definitely not complete.

Important: these eyepieces work both on microscopes with a finite tube length (160 mm, for example) and on microscopes with infinity optics. That means you can use them on the Carl Zeiss Jena Amplival microscope just as well as on the Carl Zeiss Jena NU-2 microscope.

Description of this part for the microscope.

Gleitfett grades differ in their parameters.

Gleitfett I E10 and Gleitfett II E75 were supplied with the Carl Zeiss Jena micromanipulator.
The Gleitfett III E410 offered here was supplied with the Carl Zeiss Jena Neophot-21 microscope.

This MAY suggest (although I have no proof in the form of manufacturer documentation) that the lower numbers (I and II) and the lower values of the "E" parameter (E10 and E75) were intended for finer mechanisms - and may therefore have a lower viscosity, for example. The III E410 grease, with its high "E" value, was supplied with a heavy microscope built around massive mechanics - so it may well be thicker.

This grease can of course be used on microscopes from other manufacturers as well - a few are listed in the "compatibility" section, but that is by no means a complete list.

Description of this part for the microscope.

100x
Colour correction: achromatic
Model: E. 100 1.25 oil 160/0.17
Numerical aperture N.A.: 1.25
Cover glass: yes, 0.17 mm thick
Thread: RMS
Tube length: 160 mm
Parfocal length: 45 mm (long barrel objective)
Immersion: yes, oil
Operating mode: objectives marked "E." are already CF objectives (CFN to be precise) and need no additional correction

This objective needs no additional correction from a tube lens or from the eyepieces. That makes it possible to fit it to microscopes of other makes, and in photomicrography it works in direct projection mode (it throws the image straight onto the camera sensor, with no relay optics needed).

This is the so-called black series of objectives, named after the black plastic barrel. They were used mainly in the Nikon Alphaphot YS2 microscope. That was the period when manufacturers were experimenting with a new look for microscope parts, and exactly the same black series appeared for instance in the Leitz / Leica HM-LUX 3 microscope or in the Will Wetzlar V300 / V355 / V365 microscopes.

This objective fits practically any microscope of any make, provided it has an RMS thread and supports objectives with TL = 160 mm and parfocal lengths of 33 / 37 and 45 mm. A few such microscopes are listed in the "compatibility" section, but that list is not complete.

Description of this part for the microscope.

correction: achromatic
Model: E 40 0.65 160/0.17
Numerical aperture N.A.: 0.65
Cover glass: yes, 0.17 mm thick
Thread: RMS
Tube length: 160 mm
Parfocal length: 45 mm (long barrel objective)
Immersion: no
Operating mode: objectives marked "E." are already CF objectives (CFN to be precise) and need no additional correction

This objective needs no additional correction from a tube lens or from the eyepieces. That makes it possible to fit it to microscopes of other makes, and in photomicrography it works in direct projection mode (it throws the image straight onto the camera sensor, with no relay optics needed).

This is the so-called black series of objectives, named after the black plastic barrel. They were used mainly in the Nikon Alphaphot YS2 microscope. That was the period when manufacturers were experimenting with a new look for microscope parts, and exactly the same black series appeared for instance in the Leitz / Leica HM-LUX 3 microscope or in the Will Wetzlar V300 / V355 / V365 microscopes.

This objective fits practically any microscope of any make, provided it has an RMS thread and supports objectives with TL = 160 mm and parfocal lengths of 33 / 37 and 45 mm. A few such microscopes are listed in the "compatibility" section, but that list is not complete.

Description of this part for the microscope.

be attached to a trinocular head or directly to the microscope body, even when there is no trinocular head.

Basic technical parameters of this tube:
Manufacturer: Carl Zeiss Jena
Colour: grey
Tilt angle: none, this is a straight tube
Internal optics: none - not even a deflecting prism
Mounting to the trinocular head or microscope body: ring with a dovetail
Dovetail diameter: 41 mm
Type of connection at the output: empty tube / barrel
Tube diameter at the output: 23.2 mm internal diameter
Length of the barrel / tube: 25 mm
Length adjustment: no - this is not a tube with adjustable height

Since the adapter has an empty barrel with an internal diameter of 23.2 mm on the output side, a projection eyepiece of that diameter can be placed in it, or a so-called "USB eyepiece", that is a digital microscope camera. Adapters between the 23.2 mm barrel and the M42 thread are also widely available, and from the M42 thread you can move on to any other mount: Nikon, Canon, Sony and others.

Such a tube can be mounted directly on the microscope body instead of the head. A dovetail seat with a diameter of 41.0 - 41.5 mm is found, among others, on the microscopes:
- Carl Zeiss Jena
- LOMO
- Meopta
- IOR
- Reichert Biopan
- Olympus CH

It is also often used in microscopes with a slightly larger seat, among others Zeiss West Germany, Leitz Wetzlar, PZO, Will Wetzlar, Hund Wetzlar.

The adapter can equally well be fitted onto trinocular heads, as many of them have a seat prepared for it, among others:
- the output in the trinocular tubes of the Carl Zeiss Jena Jenamed, Jenaval, Jenalumar, Jenatech, Jenavert, Jenapol microscopes
- the LOMO TRN-1 heads (ЛОМО ТРН-1), found in the LOMO Polam microscopes
- some versions of the intermediate photo tubes for the Carl Zeiss Jena Mikroval series microscopes (others require a tube screwed onto a thread)

This is not a "tube for one specific microscope model". A trinocular head can be mounted not only on the microscope it was sold with - it is therefore correct to assume that this is a universal tube, with a 41 mm dovetail on one side and a 23.2 mm barrel on the other.

The "compatibility" section gives an example list of microscopes this tube fits, although it is not a complete list.

Description of this part for the microscope.

The tube allows a camera or a camera body to be attached to a trinocular head or directly to the microscope body, even when there is no trinocular head.

Basic technical parameters of this tube:
Manufacturer: LOMO (ЛОМО)
Colour: black, rings and barrel chrome-plated
Tilt angle: none, this is a straight tube
Internal optics: none - not even a deflecting prism
Mounting to the trinocular head or microscope body: ring dovetail (male part)
Dovetail diameter: 41 mm
Type of connection at the output: empty tube / barrel
Tube diameter at the output: 23.2 mm internal diameter
Length of the barrel / tube: variable
Length adjustment: yes - the barrel is extendable, with a scale from 140 to 200 mm and a knurled locking ring

This is the version with adjustable tube length. A scale from 140 to 200 mm is engraved on the extendable barrel, with a reference line and intermediate divisions, and the setting is locked with a knurled ring. This gives two things at once. First, a continuous change of the image scale - the longer the tube, the larger the image falling on the sensor or the film, so the framing can be adjusted without changing the objective. Second, and more importantly, the ability to work with objectives computed for different optical tube lengths: 160, 170, 180, 190 and 200 mm. Carl Zeiss Jena and LOMO objectives are computed for 160 mm, Leitz Wetzlar for 170 mm, some objectives of metallurgical and measuring microscopes for 190 or 200 mm - here you simply set the barrel to the right value and the objective works under its design conditions, instead of delivering an image with residual aberration.

Since the adapter has an empty barrel with an internal diameter of 23.2 mm on the output side, a projection eyepiece of that diameter can be placed in it, or a so-called "USB eyepiece", that is a digital microscope camera. Adapters between the 23.2 mm barrel and the M42 thread are also widely available, and from the M42 thread you can move on to any other mount: Nikon, Canon, Sony and others.

Such a tube can be mounted directly on the microscope body instead of the head. A dovetail seat with a diameter of 41.0 - 41.5 mm is found, among others, on the microscopes:
- LOMO
- Carl Zeiss Jena
- Meopta
- IOR
- Reichert Biopan
- Olympus CH

It is also often used in microscopes with a slightly larger seat, among others Zeiss West Germany, Leitz Wetzlar, PZO, Will Wetzlar, Hund Wetzlar.

The "compatibility" section gives an example list of microscopes this tube fits, although it is not a complete list.

The last photo shows a LOMO Erudit microscope with this photo tube and a projection eyepiece mounted. It is an example of use.

Description of this part for the microscope.

objective:
Manufacturer: Zeiss West Germany
Engraving on the objective: Planapo 63/1,4 Oel 160/-
Manufacturer catalogue number: 46 18 40 - 9901 (also written as 461840-9901)
Magnification: 63x
Numerical aperture (N.A.): 1.4
Optical correction: plan-apochromatic
Immersion: yes, oil
Coverslip: according to the engraving on the barrel of this objective, it can be used both without a coverslip and with a standard coverslip of 0.17 mm thickness
Tube length: 160 mm
Parfocal distance: 45 mm
Thread: RMS
Specimen protection: yes, the objective has a spring-loaded mechanism preventing the slide with the specimen from being broken
Phase contrast: no - the objective has no phase rings inside
DIC: prisms allowing work in DIC mode are available on the market for this objective (sold separately)

This objective fits practically any microscope of any manufacturer, provided it has an RMS thread and accepts objectives for a 160 mm tube length with a parfocal distance of 33, 37 or 45 mm. A few such microscopes are listed as examples in the "compatibility" section, but this is not a complete list.

Description of this part for the microscope.

catalogue number: 46 78 36 (also written as 467836)
Colour: blue matt
Diameter: 32 mm
Material: glass

This is a filter that combines the functions of a blue filter and a ground glass diffuser:
- the blue filter is used to convert the yellow, warm light of the bulb into light closer to white light
- the matt filter (called a diffuser or ground glass) is used to even out the light across the whole area of the specimen and to remove the visible filament of the bulb

This does, however, have one consequence: without a visible filament it is not possible to work with illumination set up according to the Koehler method - and Zeiss mentions this in the leaflet supplied with the filter.

Very often only one or two microscope filters can be fitted in a microscope, for example one on the illuminator and the second in the holder below the condenser. The fact that the glass filter offered here is a 2-in-1 solution makes it possible to save the possible second slot for another filter.

The diameter of 32 mm is a standard with many manufacturers, which means this filter can be used with microscopes of numerous brands. The "compatibility" section lists over a dozen such microscopes, but it is not a complete list.

Description of this part for the microscope.

camera to a trinocular head or directly to the microscope stand, even when there is no trinocular head at all.

This is a black tube with an upper element (barrel) 55 mm long. Here (https://www.microscopeparts.eu/gallery/91057) you can see a functionally identical adapter, but grey and with an upper barrel only 25 mm long.

Black adapters of this kind come from the era of the Carl Zeiss Jena L stands (models Carl Zeiss Jena Lg, Lumipan, Ng, Nf or Nu). The grey ones are newer, from the era of the Mikroval series (models Carl Zeiss Jena Laboval, Eduval, Ergaval or Amplival and Docuval).

The mounting fit is of course identical, so both can be used interchangeably.

Basic technical parameters of this tube:
Manufacturer: Carl Zeiss Jena
Colour: black
Inclination angle: none, this is a straight tube
Internal optics: none - not even a deflecting prism
Mounting to a trinocular head or microscope stand: ring dovetail
Ring dovetail diameter: 41.5 mm
Type of connection at the output: empty barrel / tube
Tube diameter at the output: 23.2 mm inner diameter
Barrel / tube length: 55 mm
Tube length adjustment: no - this is not a height-adjustable tube

Because on the output side this adapter has an empty barrel with an inner diameter of 23.2 mm, you can place in it a projection eyepiece of that diameter or a so-called USB eyepiece, that is a digital microscope camera. In addition, adapters between the 23.2 mm barrel and the M42 thread are widely available, and from the M42 thread you can already move on to any other mount: Nikon, Canon, Sony and all the rest.

Such a tube can be mounted directly on the microscope stand, in place of the head. A seat for a ring dovetail of 41.0 - 41.5 mm diameter is found among others in the microscopes:
- Carl Zeiss Jena
- LOMO
- Meopta
- IOR
- Reichert Biopan
- Olympus CH
- Gamma

It is also used in microscopes with a slightly larger seat, among others Zeiss West Germany, Leitz Wetzlar, PZO, Will Wetzlar, Hund Wetzlar.

The adapter can likewise be fitted onto trinocular heads, as many of them have a seat prepared for it, among others:
- the output in the trinocular tubes of the microscopes Carl Zeiss Jena Jenamed, Jenaval, Jenalumar, Jenatech, Jenavert, Jenapol
- the LOMO TRN-1 heads (ЛОМО ТРН-1), found in LOMO Polam microscopes
- some versions of the black intermediate photo tubes for the microscopes Carl Zeiss Jena Lg / Lumipan / Nf and of the grey ones from the Mikroval series (others require a tube that screws in on a thread)

This is not a tube for one particular microscope model. A trinocular head can be mounted not only on the microscope it was sold with, so the correct assumption is that this is a universal tube with a ring dovetail of 41.5 mm diameter on one side and a barrel of 23.2 mm diameter on the other.

In the compatibility section you will find an example list of microscopes this tube fits, although that list is not complete.

Description of this part for the microscope.

Jena
Inscription: logo "Carl Zeiss Jena" and text "Q1 MADE IN DDR"
Material: aluminium
Length: 52 mm
Width: 14 mm
Mounting: the nameplate is glued on

By default such a nameplate was fitted to most Carl Zeiss Jena microscopes from the Mikroval series onwards. There is also a smaller nameplate - with the manufacturer logo only (without the "Q1 Made in DDR" text). You can see it here: https://www.microscopeparts.eu/part/00604.

Because the adhesive used to attach these plates to the microscope body loses its properties over the years, these plates very often fall off. A nameplate like this makes it possible to restore the factory appearance of a microscope using original parts.

Description of this part for the microscope.

5x
Magnification: 2.5x
Mounting diameter: 23.2 mm

Please note: this is a "CF" projection photo eyepiece, meaning "Chrome Free" (chromatic aberration free). The eyepiece itself carries no chromatic correction - for correct results it has to sit in the microscope objective. In the case of Nikon, those are the CF (CFN) and CFI objectives.

The standard 23.2 mm mounting diameter allows it to be used in the projection tubes of many microscopes from various manufacturers - both biological and stereo ones.

It has to be said that this is a very universal projection eyepiece. In the "compatibility" section I list only a small part of the microscopes this photo eyepiece can be used with.

From the experience of people I know, only testing a projection eyepiece yourself on a specific microscope setup gives you certainty about how it will behave.

With every configuration - different objectives, a different tube length, a different photo adapter, camera, DSLR, sensor size - the user will get a different result.

This applies to tests both on microscopes with finite tube length optics (for example the Nikon Labophot with 160 mm optics) and infinity optics (for example the Nikon Eclipse E600 infinity), and both on older stereo microscopes (for example the Nikon SMZ-10) and somewhat newer ones such as the Nikon SMZ-1500.

Description of this part for the microscope.

5
Magnification: 5x
Field number F.N.: 25
Mounting diameter: 23.2 mm

Please note: PLI is a series of projection eyepieces assigned to the CFI60 system (optics corrected for infinity / "I" as in "infinity"). I have, however, personally seen eyepieces from this series used on microscopes with optics corrected for a finite tube length (160 mm, to be precise).

This eyepiece contains no chromatic correction lenses, so that correction has to be built into the objective (in the case of Nikon objectives these are the CF / CFN / CFI series).

The standard 23.2 mm mounting diameter allows it to be used in the projection tubes of many microscopes from various manufacturers - both biological and stereo ones. It therefore has to be regarded as a very universal projection eyepiece. In the "compatibility" section I list only a small part of the microscopes this photo eyepiece can be used with.

From the experience of people I know, only testing a projection eyepiece yourself on a specific microscope setup gives you certainty about how it will behave.

With every configuration - different objectives, a different tube length, a different photo adapter, camera, DSLR, sensor size - the user will get a different result.

This applies to tests both on microscopes with finite tube length optics (for example the Nikon Alphaphot with 160 mm optics) and infinity optics (for example the Nikon Eclipse TS-100 infinity), and both on older stereo microscopes (for example the Nikon SMZ-2T) and somewhat newer ones such as the Nikon SMZ-18.