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Description of this part for the microscope.

08 160/-
Color correction: Planachromat
Numerical aperture: 0.08
Tube length: 160 mm
Focal length: 45 mm
Cover glass: no
Immersion: no
Thread: RMS

This lens provides an excellent, wide image, and because it doesn't require a cover glass, it's often used for macro photography and stacking.

Important - this lens is not suitable for taking photos using the "direct-projection" technique, as it requires plan-compensation optics.

A microscope objective with such low magnification is used, among others, in the following fields:
- tissue overview examination in pathomorphology
- observation of insects
- observation of minerals
- observation of inclusions in amber
- handwriting analysis
- observation of micromollusks
- and many others

Description of this part for the microscope.

Length: 45 mm
Cover Glass: yes, 0,17 mm
Immersion: no
Thread: RMS
Info: Ph = phase contrast

It can be used with any microscope from any manufacturer, along with a compatible phase contrast condenser. The microscope must be compatible, meaning it supports RMS objectives, TL = 160 mm, FL = 45 mm.

Description of this part for the microscope.

mm
Focal Length: 45 mm
Cover Glass: yes, 0,17 mm
Immersion: yes, oil
Thread: RMS
Info: Ph3 = phase contrast

It can be used with any microscope from any manufacturer, along with a compatible phase contrast condenser. The microscope must be compatible, meaning it supports RMS objectives, TL = 160 mm, FL = 45 mm.

Description of this part for the microscope.

10x
Correction: Achromat
Numerical aperture: 0.24
Tube length: 160 mm
Focal length: 45 mm
Cover glass: no
Immersion: no
Thread: RMS
Info: PI = polarization-interference objective (red ring).

Compatible with the following PZO condensers:
- PZO KPI 2
- slit condenser
PI objectives are among the components of the PZO UPI set (i.e. the so-called DIC).

VERY IMPORTANT: the marking on the objective is "PI" (in lowercase: "pi"), meaning [P]-polarization [I]-interference.
In many descriptions available on the Internet, you can find information that this is a PLAN objective. This is an ERROR caused by misinterpreting the marking as PL.

Note: another INCORRECT piece of information often found online is that these are DIC objectives. This is not true.
DIC according to Pluta (niem: DIC nach Pluta), sold by PZO, achieves the DIC effect (i.e. a 3D relief image) using standard achromatic PZO objectives (and not only those). Objectives marked PI with a red ring are not required for this.

It can be used with any microscope from any manufacturer that supports RMS objectives, TL=160 mm, FL=45 mm.

Description of this part for the microscope.

10 160/-
Color correction: Planachromat
Numerical aperture: 0.10
Tube length: 160 mm
Focal length: 45 mm
Cover glass: no
Immersion: no
Thread: RMS

This lens provides an excellent, wide image, and because it doesn't require a cover glass, it's often used for macro photography and stacking.

Important - this lens is not suitable for taking photos using the "direct-projection" technique, as it requires plan-compensation optics.

A microscope objective with such low magnification is used, among others, in the following fields:
- tissue overview examination in pathomorphology
- observation of insects
- observation of minerals
- observation of inclusions in amber
- handwriting analysis
- observation of micromollusks
- and many others

It can be used with any microscope from any manufacturer. Of course, the microscope must be compatible, i.e. support RMS objectives, TL=160 mm, FL=45 mm.

Description of this part for the microscope.

Color correction: Planachromat
Numerical aperture: 0.25
Tube length: 160 mm
Focal length: 45 mm
Cover glass: no
Immersion: no
Thread: RMS

It can be used with any microscope from any manufacturer. Of course, the microscope must be compatible, i.e. support RMS objectives, TL=160 mm, FL=45 mm.

Description of this part for the microscope.

17
Color correction: Planachromat
Numerical aperture: 0.32
Tube length: 160 mm
Focal length: 45 mm
Cover glass: yes, 0,17 mm
Immersion: no
Thread: RMS

It can be used with any microscope from any manufacturer. Of course, the microscope must be compatible, i.e. support RMS objectives, TL=160 mm, FL=45 mm.

Description of this part for the microscope.

17
Color correction: Planachromat
Numerical aperture: 0.65
Tube length: 160 mm
Focal length: 45 mm
Cover glass: yes, 0,17 mm
Immersion: no
Thread: RMS

It can be used with any microscope from any manufacturer. Of course, the microscope must be compatible, i.e. support RMS objectives, TL=160 mm, FL=45 mm.

Description of this part for the microscope.

17
Color correction: Planachromat
Numerical aperture: 1,25
Tube length: 160 mm
Focal length: 45 mm
Cover glass: yes, 0,17 mm
Immersion: yes, oil
Thread: RMS
Info: HI = Homogene Immersion, also oil immersion

It can be used with any microscope from any manufacturer. Of course, the microscope must be compatible, i.e. support RMS objectives, TL=160 mm, FL=45 mm.

Description of this part for the microscope.

3x
Model: Semiplan 6.3/0.16 160/-
Color correction: Semi-Planachromat
Numerical aperture: 0.16
Tube length: 160 mm
Focal length: 45 mm
Cover glass: no
Immersion: no
Thread: RMS

This is one of the most commonly used microscope objectives for macro photography and focus stacking.

Important - this objective IS SUITABLE for taking images using the "direct-projection" technique, as it does not require plan-compensating optics.

It can be used with any microscope from any manufacturer. Of course, the microscope must be compatible, meaning it supports RMS objectives, TL=160 mm, FL=45 mm.

Description of this part for the microscope.

Manufacturer: Lomo
Magnification: 20x
Model: 20 0.40 P
Numerical aperture: 0.40
Tube length: 160 mm
Focal length: 33 mm
Cover glass: yes, 0.17 mm
Thread: RMS + Pol adapter
Info: P = Polarization

This is an objective designed for transmitted light work with a cover glass.

The RMS thread objective is mounted on a dedicated slide-in adapter that allows it to be used in Lomo MIN series and Lomo Polam series microscopes. Of course, the objective can be unscrewed and another objective can be mounted in the adapter.

The objective itself, once removed from the adapter, is a standard objective that can be used in virtually any microscope with an RMS thread. It is a so-called "short objective" (focal length 33 mm). When a 12 mm adapter is added, the total length becomes 45 mm, allowing it to be used with newer microscopes from any manufacturer.

Lomo polarization objectives with RMS thread can be installed in an RMS-M27 adapter and then used in microscopes such as Lomo Biolam M, Lomo Biolam I, Lomo Lumam, or Lomo ML-2. I have also frequently seen such objectives used in Zeiss Axio series microscopes with M27 thread.

The Pol adapter also allows precise centering of the objective relative to the entire optical path - this requires tools (keys / tools).

Info: I have repeatedly seen information stating that this RMS-Pol adapter is compatible with Leitz Wetzlar polarization microscopes - however, I have not personally verified this.

In summary, this objective is dedicated to Lomo MIN-1, MIN-4, MIN-5, MIN-6, MIN-8, Lomo Polam R-113, Polam L-211, Polam L-213, Polam R-311, Polam LMPR microscopes - but it is practically compatible with any microscope from any manufacturer that supports 160 mm objectives.

Description of this part for the microscope.

Note - this model of microscope objective is very often used for macro photography and focus stacking. More information on this can be found at the end of this description.

Manufacturer: Lomo
Magnification: 9x
Model: PLANACHR 9x0.20 P
Numerical aperture: 0.20
Optical correction: planachromatic (flat field)
Tube length: 160 mm
Focal length: 33 mm
Cover glass: optional, can be used but is not required
Thread: RMS + Pol adapter
Info: P = Polarization

The RMS thread objective is mounted on a dedicated slide-in adapter that allows it to be used in Lomo MIN series and Lomo Polam series microscopes. Of course, the objective can be unscrewed and another objective can be mounted into the adapter.

The objective itself, once removed from the adapter, is a standard objective that can be used in virtually any microscope with an RMS thread. It is a so-called "short objective" (focal length 33 mm). When a 12 mm adapter is added, the total length becomes 45 mm, allowing it to be used with newer microscopes from any manufacturer.

Lomo polarization objectives with RMS thread can be installed in an RMS-M27 adapter and then used in microscopes such as Lomo Biolam M, Lomo Biolam I, Lomo Lumam, or Lomo ML-2. I have also frequently seen such objectives used in Zeiss Axio series microscopes with M27 thread.

The Pol adapter also allows precise centering of the objective relative to the entire optical path - this requires tools (keys / tools).

Info: I have repeatedly seen information stating that this RMS-Pol adapter is compatible with Leitz Wetzlar polarization microscopes - however, I have not personally verified this.

In summary, this objective is dedicated to Lomo MIN-1, MIN-4, MIN-5, MIN-6, MIN-8, Lomo Polam R-113, Polam L-211, Polam L-213, Polam R-311, Polam LMPR microscopes - but it is practically compatible with any microscope from any manufacturer that supports 160 mm objectives.

Additionally, this objective is very often used for macro photography even without a microscope. This objective does not require a cover glass and works in the "direct-projection" technique, meaning it can be connected directly to a camera without additional optics. This allows achieving a very high magnification scale and great depth of field across the entire image when using focus stacking. The fact that this is a polarization objective (i.e. stress-free optics without internal glass tensions) further improves image quality at high magnifications.

Description of this part for the microscope.

Note - this model of microscope objective is very often used for macro photography and focus stacking. More information on this can be found at the end of this description.

Manufacturer: Lomo
Magnification: 3,7x
Model: 3,7 0,11
Numerical aperture: 0.11
Tube length: 160 mm
Focal length: 33 mm
Cover glass: optional, can be used but is not required
Thread: RMS + Pol adapter
Info: P = Polarization

The RMS thread objective is mounted on a dedicated slide-in adapter that allows it to be used in Lomo MIN series and Lomo Polam series microscopes. Of course, the objective can be unscrewed and another objective can be mounted into the adapter.

The objective itself, once removed from the adapter, is a standard objective that can be used in virtually any microscope with an RMS thread. It is a so-called "short objective" (focal length 33 mm). When a 12 mm adapter is added, the total length becomes 45 mm, allowing it to be used with newer microscopes from any manufacturer.

Lomo polarization objectives with RMS thread can be installed in an RMS-M27 adapter and then used in microscopes such as Lomo Biolam M, Lomo Biolam I, Lomo Lumam, or Lomo ML-2. I have also frequently seen such objectives used in Zeiss Axio series microscopes with M27 thread.

The Pol adapter also allows precise centering of the objective relative to the entire optical path - this requires tools (keys / tools).

Info: I have repeatedly seen information stating that this RMS-Pol adapter is compatible with Leitz Wetzlar polarization microscopes - however, I have not personally verified this.

In summary, this objective is dedicated to Lomo MIN-1, MIN-4, MIN-5, MIN-6, MIN-8, Lomo Polam R-113, Polam L-211, Polam L-213, Polam R-311, Polam LMPR microscopes - but it is practically compatible with any microscope from any manufacturer that supports 160 mm objectives.

Additionally, this objective is very often used for macro photography even without a microscope. This objective does not require a cover glass and works in the "direct-projection" technique, meaning it can be connected directly to a camera without additional optics. This allows achieving a very high magnification scale and great depth of field across the entire image when using focus stacking. The fact that this is a polarization objective (i.e. stress-free optics without internal glass tensions) further improves image quality at high magnifications. Info: https://www.closeuphotography.com/lomo-3-7x-objective/2017/12/24/lomo-37x-011-microscope-objective

Description of this part for the microscope.

1017
Magnification: 4x
Numerical aperture N.A.: 0.12
Cover glass: none
Thread: RMS
Tube length: infinity
Immersion: no

This objective is part of the standard equipment of the AO One-fifty microscope, but it is fully compatible with the AO One-ten model and allows the AO One-ten to be upgraded with plan achromatic optics. The AO Reichert Microstar 110 microscope is in fact an AO One-ten sold under a different name.

Description of this part for the microscope.

1023
Magnification: 40x
Numerical aperture N.A.: 0.66
Cover glass: yes, 0,17 mm
Thread: RMS
Tube length: infinity
Immersion: no

This objective is part of the standard equipment of the AO One-fifty microscope, but it is fully compatible with the AO One-ten model and allows the AO One-ten to be upgraded with plan achromatic optics. The AO Reichert Microstar 110 microscope is in fact an AO One-ten sold under a different name.

Description of this part for the microscope.

35 160/0.17
Manufacturer part number: 46 05 10-9906 (alternative notation: 460510 - 9906)
Color correction: Planachromat
Numerical aperture N.A.: 0.35
Tube length: 160 mm
Focal length: 45 mm
Cover glass: yes, 0.17 mm thickness
Immersion: no (dry objective)
Thread: RMS

Description of this part for the microscope.

65 160/0.17
Manufacturer part number: 46 07 10-9904 (alternative notation: 460710 - 9904)
Color correction: Planachromat
Numerical aperture N.A.: 0.65
Tube length: 160 mm
Focal length: 45 mm
Cover glass: yes, 0.17 mm thickness
Immersion: no (dry objective)
Thread: RMS

Description of this part for the microscope.

Wetzlar
Model: 160/0,17 EF 100/1.25 OEL PHACO 3
Magnification: 100x
Correction: semi-plan achromat
Numerical aperture: 1.25
Tube length: 160 mm
Focal length: 45 mm
Cover glass: yes, 0.17 mm
Immersion: yes, oil
Thread: RMS
Specimen protection: yes - spring-loaded
Info: PHACO 3 = phase contrast

Some important information about this objective:
- the designation "EF" means "Extended Field" - this is a semi-plan achromatic objective with an 18 mm field of view, which should be taken into account when selecting suitable eyepieces
- the semi-plan achromatic correction is "almost flat" - it is no longer an achromat, but not yet a plan achromat
- this objective can be used in brightfield, but phase contrast operation requires a compatible phase contrast condenser or a slide (slider) with a phase ring diaphragm
- the designation PHACO 3 means that for phase contrast operation a phase contrast condenser set to ring "3" should be used (the equivalent setting used by Zeiss is called "Ph3")
- this objective can be used with microscopes designed for objectives with a 170 mm tube length, but in such a case the optical path must be extended by 10 mm - most commonly by pulling the eyepieces out of the tubes by 10 mm

This objective can be used with any microscope from any manufacturer together with a compatible phase contrast condenser. Naturally, the microscope must be compatible, i.e. it must support RMS objectives, TL=160 mm and FL=45 mm - or have adapters that make such adaptation possible (for example thread adapters).

Description of this part for the microscope.

ameters:
Manufacturer: Lomo
Model: PLAN 25x0.50 P
Original designation: ЛОМО ПЛАН 25х0,50 П
Magnification: 25x
Numerical aperture N.A.: 0.50
Tube length: 160 mm
Cover glass: yes, 0.17 mm thickness
Immersion oil: no - this is a dry objective
Focal length: 45 mm
Thread type: RMS
Additional designation: P (Cyrillic: П) indicates that this objective is designed for use with polarized light
Special features: built-in iris diaphragm
Year of manufacture: 1976

This objective has a standard RMS thread and TL=160 mm, which means it can be used with the vast majority of microscopes from virtually any manufacturer, including Olympus, Zeiss, Nikon, PZO, Carl Zeiss Jena, Reichert, Wild and many others.

The built-in iris diaphragm allows, among other things, precise centering of the objective, because in a polarizing microscope the following diaphragms may operate simultaneously:
- field diaphragm in the illuminator
- aperture diaphragm (or two aperture diaphragms) in the condenser
- iris diaphragm in the microscope objective
- iris diaphragm in the eyepiece head
and accurate centering of all of them relative to each other is very important in polarized-light observations.

Objectives marked "P" / "Pol" feature a special construction in which the lenses are mounted and cemented in a way that prevents mechanical stress. They can be used both for conventional brightfield microscopy and for observations in polarized light.

Description of this part for the microscope.

3x
Model: Semiplan 6.3/0.16 160/-
Serial number: 039 562 (039562)
Color correction: Semi-Planachromat
Numerical aperture: 0.16
Tube length: 160 mm
Focal length: 45 mm
Cover glass: no
Immersion: no
Thread: RMS

This is one of the most commonly used microscope objectives for macro photography and focus stacking.

Important - this objective IS SUITABLE for taking images using the "direct-projection" technique, as it does not require plan-compensating optics.

It can be used with any microscope from any manufacturer. Of course, the microscope must be compatible, meaning it supports RMS objectives, TL=160 mm, FL=45 mm.

Description of this part for the microscope.

for inverted and upright biological microscopes, made in Japan.

Basic objective parameters:
Manufacturer: Nikon, Japan
Model: Ph3 40 DL 0.55 LWD 160/0-2
Magnification: 40x
Numerical aperture (N.A.): 0.55
Optical correction: achromat
Phase contrast: Ph3 DL - Ph3 indicates the size of the phase ring (requires a matching Ph3 annulus in the condenser), DL (Dark Low) means dark, i.e. positive phase contrast - the object appears darker than the background
Working distance (WD): ~2.1 - 2.5 mm - LWD (Long Working Distance)
Correction collar: compensates for cover glass or vessel bottom thickness in the range 0 - 2.0 mm
Cover glass: the objective can be used without a cover glass, with a standard cover glass approx. 0.17 mm thick, and also with specimens observed through the bottom of a Petri dish up to 2.0 mm thick
Immersion: no (dry objective)
Tube length: 160 mm
Parfocal length: 45 mm
Thread: RMS
Optics: CF (Nikon CF-160) - requires no compensating eyepiece and allows photography by direct projection

This objective is suitable for:
- inverted microscopes - observation through the bottom of a Petri dish or cell culture flask
- upright biological microscopes - observation of specimens with or without a cover glass

Phase contrast work requires a compatible phase contrast condenser or a slider with a phase contrast annulus.

Commonly used, inexpensive cover glasses are 0.17 mm thick mainly in theory. Even the standard for class #1.5 allows a range of 0.16 - 0.19 mm, and in cheap Chinese cover glasses you will find in practice pieces from approx. 0.13 to 0.25 mm, often not matching the thickness stated on the box. Only #1.5H (high precision) cover glasses have a guaranteed thickness of 0.170 mm with a tolerance of +/- 0.005 mm. Variation in cover glass thickness clearly degrades image contrast and resolution (spherical aberration). The correction collar fitted to the objective offered here lets you adjust the optics to the combined thickness of the cover glass and the medium layer beneath it. This compensates for most of the variation found in practice.

In addition, the correction collar of this objective can be set to "0" for work without a cover glass, or to large values (up to 2.0 mm) to adapt the optics to observation through a microscope slide (0.8 - 1.2 mm), a glass Petri dish (usually 1.5 - 2.0 mm - thicker dishes are outside the collar range) or a plastic Petri dish (usually approx. 1 mm, in the range 0.8 - 1.2 mm).

This objective fits practically any microscope from any manufacturer, provided it has an RMS thread and uses objectives for 160 mm tube length with a parfocal length of 33, 37 or 45 mm. Some example microscopes are listed in the "compatibility" section, but the list is not complete.

Description of this part for the microscope.

objective:
Manufacturer: Zeiss West Germany
Engraving on the objective: Planapo 63/1,4 Oel 160/-
Manufacturer catalogue number: 46 18 40 - 9901 (also written as 461840-9901)
Magnification: 63x
Numerical aperture (N.A.): 1.4
Optical correction: plan-apochromatic
Immersion: yes, oil
Coverslip: according to the engraving on the barrel of this objective, it can be used both without a coverslip and with a standard coverslip of 0.17 mm thickness
Tube length: 160 mm
Parfocal distance: 45 mm
Thread: RMS
Specimen protection: yes, the objective has a spring-loaded mechanism preventing the slide with the specimen from being broken
Phase contrast: no - the objective has no phase rings inside
DIC: prisms allowing work in DIC mode are available on the market for this objective (sold separately)

This objective fits practically any microscope of any manufacturer, provided it has an RMS thread and accepts objectives for a 160 mm tube length with a parfocal distance of 33, 37 or 45 mm. A few such microscopes are listed as examples in the "compatibility" section, but this is not a complete list.