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Description of this part for the microscope.

and darkfield, but it is also suitable for transmitted-light brightfield observation.

Nikon TU Plan Fluor objectives are frequently used for macrophotography and ultra-macrophotography. When combined with a Nikon tube lens (Nikon tube lens) or a Rodenstock Apo-Gerogon 210 mm lens, they provide outstanding image quality when connected to a camera.

Basic specifications of the Nikon CFI TU Plan Fluor 5x/0.15 microscope objective:
Manufacturer: Nikon
Manufacturer catalogue number: MUE42050
Marking on the objective: TU Plan Fluor 5x/0.15 A ∞/0 EPI D OFN25 WD18 MUE42050
Magnification: 5x
Numerical aperture N.A.: 0.15
Colour correction: Plan Fluor
Optical correction: infinity
Infinity system: CFI 60
Field number OFN: OFN25
Working distance WD: 18 mm
Cover glass: not required
Immersion: no - this is a dry objective
Operating mode in reflected-light EPI microscopy: brightfield (BF) or darkfield (DF)
Thread: M32
Compatibility with microscopes equipped with an M25-thread nosepiece: yes, by using the MXA23017 adapter or by replacing the nosepiece with the Nikon L-NBD5 or LV-NBD5 model

This objective is compatible with microscopes designed for reflected-light EPI microscopy and equipped with an M32-thread nosepiece, for example:
- Nikon Eclipse L
- Nikon Eclipse LV
- Nikon MM-400 and MM-800 toolmakers microscopes

This objective is also compatible with microscopes in which the M25-thread nosepiece can be replaced with an M32-thread objective nosepiece, namely the Nikon L-NBD5 or LV-NBD5. These include, for example:
- Nikon Eclipse E600
- Nikon Eclipse E800
- Nikon Eclipse 50i / 55i
- Nikon Eclipse 80i / 90i
- Nikon Eclipse Ci
- Nikon Eclipse Ni
A suitable reflected-light EPI illuminator can also be installed on these microscopes to take full advantage of the capabilities of this objective.

For other Nikon CFI60 microscope models, for example:
- Eclipse E200
- Eclipse E400
- Eclipse Si
- Eclipse TI / TE / TS series inverted microscopes
the MXA23017 adapter can be used, allowing M32-thread objectives to be mounted in an M25-thread nosepiece - therefore the objective presented here can also be used.

Description of this part for the microscope.

and darkfield, but it is also suitable for transmitted-light brightfield observation.

Nikon TU Plan Fluor objectives are frequently used for macrophotography and ultra-macrophotography. When combined with a Nikon tube lens (Nikon tube lens) or a Rodenstock Apo-Gerogon 210 mm lens, they provide outstanding image quality when connected to a camera.

Basic specifications of the Nikon CFI TU Plan Fluor 20x/0.45 microscope objective:
Manufacturer: Nikon
Manufacturer catalogue number: MUE42200
Marking on the objective: TU Plan Fluor 20x/0.45 A ∞/0 EPI D OFN25 WD4,5 MUE42200
Magnification: 20x
Numerical aperture N.A.: 0.45
Colour correction: Plan Fluor
Optical correction: infinity
Infinity system: CFI 60
Field number OFN: OFN25
Working distance WD: 4,5 mm
Cover glass: not required
Immersion: no - this is a dry objective
Operating mode in reflected-light EPI microscopy: brightfield (BF) or darkfield (DF)
Thread: M32
Compatibility with microscopes equipped with an M25-thread nosepiece: yes, by using the MXA23017 adapter or by replacing the nosepiece with the Nikon L-NBD5 or LV-NBD5 model

This objective is compatible with microscopes designed for reflected-light EPI microscopy and equipped with an M32-thread nosepiece, for example:
- Nikon Eclipse L
- Nikon Eclipse LV
- Nikon MM-400 and MM-800 toolmakers microscopes

This objective is also compatible with microscopes in which the M25-thread nosepiece can be replaced with an M32-thread objective nosepiece, namely the Nikon L-NBD5 or LV-NBD5. These include, for example:
- Nikon Eclipse E600
- Nikon Eclipse E800
- Nikon Eclipse 50i / 55i
- Nikon Eclipse 80i / 90i
- Nikon Eclipse Ci
- Nikon Eclipse Ni
A suitable reflected-light EPI illuminator can also be installed on these microscopes to take full advantage of the capabilities of this objective.

For other Nikon CFI60 microscope models, for example:
- Eclipse E200
- Eclipse E400
- Eclipse Si
- Eclipse TI / TE / TS series inverted microscopes
the MXA23017 adapter can be used, allowing M32-thread objectives to be mounted in an M25-thread nosepiece - therefore the objective presented here can also be used.

Description of this part for the microscope.

and darkfield, but it is also suitable for transmitted-light brightfield observation.

Nikon TU Plan Fluor objectives are frequently used for macrophotography and ultra-macrophotography. When combined with a Nikon tube lens (Nikon tube lens) or a Rodenstock Apo-Gerogon 210 mm lens, they provide outstanding image quality when connected to a camera.

Basic specifications of the Nikon CFI TU Plan Fluor 50x/0.80 microscope objective:
Manufacturer: Nikon
Manufacturer catalogue number: MUE42500
Marking on the objective: TU Plan Fluor 50x/0.80 A ∞/0 EPI D OFN25 WD1,0 MUE42500
Magnification: 50x
Numerical aperture N.A.: 0.80
Colour correction: Plan Fluor
Optical correction: infinity
Infinity system: CFI 60
Field number OFN: OFN25
Working distance WD: 1,0 mm
Cover glass: not required
Immersion: no - this is a dry objective
Operating mode in reflected-light EPI microscopy: brightfield (BF) or darkfield (DF)
Thread: M32
Compatibility with microscopes equipped with an M25-thread nosepiece: yes, by using the MXA23017 adapter or by replacing the nosepiece with the Nikon L-NBD5 or LV-NBD5 model

This objective is compatible with microscopes designed for reflected-light EPI microscopy and equipped with an M32-thread nosepiece, for example:
- Nikon Eclipse L
- Nikon Eclipse LV
- Nikon MM-400 and MM-800 toolmakers microscopes

This objective is also compatible with microscopes in which the M25-thread nosepiece can be replaced with an M32-thread objective nosepiece, namely the Nikon L-NBD5 or LV-NBD5. These include, for example:
- Nikon Eclipse E600
- Nikon Eclipse E800
- Nikon Eclipse 50i / 55i
- Nikon Eclipse 80i / 90i
- Nikon Eclipse Ci
- Nikon Eclipse Ni
A suitable reflected-light EPI illuminator can also be installed on these microscopes to take full advantage of the capabilities of this objective.

For other Nikon CFI60 microscope models, for example:
- Eclipse E200
- Eclipse E400
- Eclipse Si
- Eclipse TI / TE / TS series inverted microscopes
the MXA23017 adapter can be used, allowing M32-thread objectives to be mounted in an M25-thread nosepiece - therefore the objective presented here can also be used.

Description of this part for the microscope.

and darkfield, but it is also suitable for transmitted-light brightfield observation.

Nikon TU Plan Fluor objectives are frequently used for macrophotography and ultra-macrophotography. When combined with a Nikon tube lens (Nikon tube lens) or a Rodenstock Apo-Gerogon 210 mm lens, they provide outstanding image quality when connected to a camera.

Basic specifications of the Nikon CFI TU Plan Fluor 100x/0.90 microscope objective:
Manufacturer: Nikon
Manufacturer catalogue number: MUE42900
Marking on the objective: TU Plan Fluor 100x/0.90 A ∞/0 EPI D OFN25 WD1,0 MUE42900
Magnification: 100x
Numerical aperture N.A.: 0.90
Colour correction: Plan Fluor
Optical correction: infinity
Infinity system: CFI 60
Field number OFN: OFN25
Working distance WD: 1,0 mm
Cover glass: not required
Immersion: no - this is a dry objective
Operating mode in reflected-light EPI microscopy: brightfield (BF) or darkfield (DF)
Thread: M32
Compatibility with microscopes equipped with an M25-thread nosepiece: yes, by using the MXA23017 adapter or by replacing the nosepiece with the Nikon L-NBD5 or LV-NBD5 model

This objective is compatible with microscopes designed for reflected-light EPI microscopy and equipped with an M32-thread nosepiece, for example:
- Nikon Eclipse L
- Nikon Eclipse LV
- Nikon MM-400 and MM-800 toolmakers microscopes

This objective is also compatible with microscopes in which the M25-thread nosepiece can be replaced with an M32-thread objective nosepiece, namely the Nikon L-NBD5 or LV-NBD5. These include, for example:
- Nikon Eclipse E600
- Nikon Eclipse E800
- Nikon Eclipse 50i / 55i
- Nikon Eclipse 80i / 90i
- Nikon Eclipse Ci
- Nikon Eclipse Ni
A suitable reflected-light EPI illuminator can also be installed on these microscopes to take full advantage of the capabilities of this objective.

For other Nikon CFI60 microscope models, for example:
- Eclipse E200
- Eclipse E400
- Eclipse Si
- Eclipse TI / TE / TS series inverted microscopes
the MXA23017 adapter can be used, allowing M32-thread objectives to be mounted in an M25-thread nosepiece - therefore the objective presented here can also be used.

Description of this part for the microscope.

for inverted and upright biological microscopes, made in Japan.

Basic objective parameters:
Manufacturer: Nikon, Japan
Model: Ph3 40 DL 0.55 LWD 160/0-2
Magnification: 40x
Numerical aperture (N.A.): 0.55
Optical correction: achromat
Phase contrast: Ph3 DL - Ph3 indicates the size of the phase ring (requires a matching Ph3 annulus in the condenser), DL (Dark Low) means dark, i.e. positive phase contrast - the object appears darker than the background
Working distance (WD): ~2.1 - 2.5 mm - LWD (Long Working Distance)
Correction collar: compensates for cover glass or vessel bottom thickness in the range 0 - 2.0 mm
Cover glass: the objective can be used without a cover glass, with a standard cover glass approx. 0.17 mm thick, and also with specimens observed through the bottom of a Petri dish up to 2.0 mm thick
Immersion: no (dry objective)
Tube length: 160 mm
Parfocal length: 45 mm
Thread: RMS
Optics: CF (Nikon CF-160) - requires no compensating eyepiece and allows photography by direct projection

This objective is suitable for:
- inverted microscopes - observation through the bottom of a Petri dish or cell culture flask
- upright biological microscopes - observation of specimens with or without a cover glass

Phase contrast work requires a compatible phase contrast condenser or a slider with a phase contrast annulus.

Commonly used, inexpensive cover glasses are 0.17 mm thick mainly in theory. Even the standard for class #1.5 allows a range of 0.16 - 0.19 mm, and in cheap Chinese cover glasses you will find in practice pieces from approx. 0.13 to 0.25 mm, often not matching the thickness stated on the box. Only #1.5H (high precision) cover glasses have a guaranteed thickness of 0.170 mm with a tolerance of +/- 0.005 mm. Variation in cover glass thickness clearly degrades image contrast and resolution (spherical aberration). The correction collar fitted to the objective offered here lets you adjust the optics to the combined thickness of the cover glass and the medium layer beneath it. This compensates for most of the variation found in practice.

In addition, the correction collar of this objective can be set to "0" for work without a cover glass, or to large values (up to 2.0 mm) to adapt the optics to observation through a microscope slide (0.8 - 1.2 mm), a glass Petri dish (usually 1.5 - 2.0 mm - thicker dishes are outside the collar range) or a plastic Petri dish (usually approx. 1 mm, in the range 0.8 - 1.2 mm).

This objective fits practically any microscope from any manufacturer, provided it has an RMS thread and uses objectives for 160 mm tube length with a parfocal length of 33, 37 or 45 mm. Some example microscopes are listed in the "compatibility" section, but the list is not complete.

Description of this part for the microscope.

100x
Colour correction: achromatic
Model: E. 100 1.25 oil 160/0.17
Numerical aperture N.A.: 1.25
Cover glass: yes, 0.17 mm thick
Thread: RMS
Tube length: 160 mm
Parfocal length: 45 mm (long barrel objective)
Immersion: yes, oil
Operating mode: objectives marked "E." are already CF objectives (CFN to be precise) and need no additional correction

This objective needs no additional correction from a tube lens or from the eyepieces. That makes it possible to fit it to microscopes of other makes, and in photomicrography it works in direct projection mode (it throws the image straight onto the camera sensor, with no relay optics needed).

This is the so-called black series of objectives, named after the black plastic barrel. They were used mainly in the Nikon Alphaphot YS2 microscope. That was the period when manufacturers were experimenting with a new look for microscope parts, and exactly the same black series appeared for instance in the Leitz / Leica HM-LUX 3 microscope or in the Will Wetzlar V300 / V355 / V365 microscopes.

This objective fits practically any microscope of any make, provided it has an RMS thread and supports objectives with TL = 160 mm and parfocal lengths of 33 / 37 and 45 mm. A few such microscopes are listed in the "compatibility" section, but that list is not complete.

Description of this part for the microscope.

correction: achromatic
Model: E 40 0.65 160/0.17
Numerical aperture N.A.: 0.65
Cover glass: yes, 0.17 mm thick
Thread: RMS
Tube length: 160 mm
Parfocal length: 45 mm (long barrel objective)
Immersion: no
Operating mode: objectives marked "E." are already CF objectives (CFN to be precise) and need no additional correction

This objective needs no additional correction from a tube lens or from the eyepieces. That makes it possible to fit it to microscopes of other makes, and in photomicrography it works in direct projection mode (it throws the image straight onto the camera sensor, with no relay optics needed).

This is the so-called black series of objectives, named after the black plastic barrel. They were used mainly in the Nikon Alphaphot YS2 microscope. That was the period when manufacturers were experimenting with a new look for microscope parts, and exactly the same black series appeared for instance in the Leitz / Leica HM-LUX 3 microscope or in the Will Wetzlar V300 / V355 / V365 microscopes.

This objective fits practically any microscope of any make, provided it has an RMS thread and supports objectives with TL = 160 mm and parfocal lengths of 33 / 37 and 45 mm. A few such microscopes are listed in the "compatibility" section, but that list is not complete.