Home page of the microscope service parts, repair and spare parts online catalog - Sermik-Oplatek Mikroskopservice Dresden f.n.n.Z

Description of this part for the microscope.

Description of this part for the microscope.

and the scale can be focused or removed from the optical path. The "regular" eyepiece is an IOR 8x. The measuring eyepiece is designated IOR (+) (crosshair logo) M8x (M = measuring).

Description of this part for the microscope.

37 mm)
- four phase contrast objectives
- MIR-4 auxiliary microscope (23,2 mm eyepiece)
- wooden case

Four phase contrast objectives:
- Lomo 10x 0.30 F
- Lomo 20x 0.40 F
- Lomo 40x 0.65 F
- Lomo MI 90x 1.25 F
- thread: RMS
- tube length: 160 mm
- focal length: 33 mm

Can be used with, among others: With Lomo microscopes:
- Biolam (Bresser Biolam)
- Biolam I
- MBB 1A
- MBR
- MBI
- MBI-11
- and others

This set is often used with Carl Zeiss Jena microscopes. To use it with microscopes from dozens of other brands, a condenser adapter is used, changing the condenser mounting diameter to 39.5 mm, and adapters changing the focal length of the objectives to 45 mm. It can then be used with PZO, Olympus, Nikon, Hund Wetzlar, Will Wetzlar, and most other microscopes with a TL of 160 mm.

Description of this part for the microscope.

08 160/-
Color correction: Planachromat
Numerical aperture: 0.08
Tube length: 160 mm
Focal length: 45 mm
Cover glass: no
Immersion: no
Thread: RMS

This lens provides an excellent, wide image, and because it doesn't require a cover glass, it's often used for macro photography and stacking.

Important - this lens is not suitable for taking photos using the "direct-projection" technique, as it requires plan-compensation optics.

A microscope objective with such low magnification is used, among others, in the following fields:
- tissue overview examination in pathomorphology
- observation of insects
- observation of minerals
- observation of inclusions in amber
- handwriting analysis
- observation of micromollusks
- and many others

Description of this part for the microscope.

Length: 33 mm
Cover Glass: 0.17 mm
Immersion: no
Thread: RMS

It can be used with any microscope from any manufacturer. The microscope must be compatible, meaning it supports RMS objectives, TL = 160 mm, FL = 33 mm.

After installing the 12 mm adapter, this objective lens will have FL = 45 mm.

Description of this part for the microscope.

Length: 45 mm
Cover Glass: yes, 0,17 mm
Immersion: no
Thread: RMS
Info: Ph = phase contrast

It can be used with any microscope from any manufacturer, along with a compatible phase contrast condenser. The microscope must be compatible, meaning it supports RMS objectives, TL = 160 mm, FL = 45 mm.

Description of this part for the microscope.

mm
Focal Length: 45 mm
Cover Glass: yes, 0,17 mm
Immersion: yes, oil
Thread: RMS
Info: Ph3 = phase contrast

It can be used with any microscope from any manufacturer, along with a compatible phase contrast condenser. The microscope must be compatible, meaning it supports RMS objectives, TL = 160 mm, FL = 45 mm.

Description of this part for the microscope.

10x
Correction: Achromat
Numerical aperture: 0.24
Tube length: 160 mm
Focal length: 45 mm
Cover glass: no
Immersion: no
Thread: RMS
Info: PI = polarization-interference objective (red ring).

Compatible with the following PZO condensers:
- PZO KPI 2
- slit condenser
PI objectives are among the components of the PZO UPI set (i.e. the so-called DIC).

VERY IMPORTANT: the marking on the objective is "PI" (in lowercase: "pi"), meaning [P]-polarization [I]-interference.
In many descriptions available on the Internet, you can find information that this is a PLAN objective. This is an ERROR caused by misinterpreting the marking as PL.

Note: another INCORRECT piece of information often found online is that these are DIC objectives. This is not true.
DIC according to Pluta (niem: DIC nach Pluta), sold by PZO, achieves the DIC effect (i.e. a 3D relief image) using standard achromatic PZO objectives (and not only those). Objectives marked PI with a red ring are not required for this.

It can be used with any microscope from any manufacturer that supports RMS objectives, TL=160 mm, FL=45 mm.

Description of this part for the microscope.

10 160/-
Color correction: Planachromat
Numerical aperture: 0.10
Tube length: 160 mm
Focal length: 45 mm
Cover glass: no
Immersion: no
Thread: RMS

This lens provides an excellent, wide image, and because it doesn't require a cover glass, it's often used for macro photography and stacking.

Important - this lens is not suitable for taking photos using the "direct-projection" technique, as it requires plan-compensation optics.

A microscope objective with such low magnification is used, among others, in the following fields:
- tissue overview examination in pathomorphology
- observation of insects
- observation of minerals
- observation of inclusions in amber
- handwriting analysis
- observation of micromollusks
- and many others

It can be used with any microscope from any manufacturer. Of course, the microscope must be compatible, i.e. support RMS objectives, TL=160 mm, FL=45 mm.

Description of this part for the microscope.

Color correction: Planachromat
Numerical aperture: 0.25
Tube length: 160 mm
Focal length: 45 mm
Cover glass: no
Immersion: no
Thread: RMS

It can be used with any microscope from any manufacturer. Of course, the microscope must be compatible, i.e. support RMS objectives, TL=160 mm, FL=45 mm.

Description of this part for the microscope.

17
Color correction: Planachromat
Numerical aperture: 0.32
Tube length: 160 mm
Focal length: 45 mm
Cover glass: yes, 0,17 mm
Immersion: no
Thread: RMS

It can be used with any microscope from any manufacturer. Of course, the microscope must be compatible, i.e. support RMS objectives, TL=160 mm, FL=45 mm.

Description of this part for the microscope.

17
Color correction: Planachromat
Numerical aperture: 0.65
Tube length: 160 mm
Focal length: 45 mm
Cover glass: yes, 0,17 mm
Immersion: no
Thread: RMS

It can be used with any microscope from any manufacturer. Of course, the microscope must be compatible, i.e. support RMS objectives, TL=160 mm, FL=45 mm.

Description of this part for the microscope.

17
Color correction: Planachromat
Numerical aperture: 1,25
Tube length: 160 mm
Focal length: 45 mm
Cover glass: yes, 0,17 mm
Immersion: yes, oil
Thread: RMS
Info: HI = Homogene Immersion, also oil immersion

It can be used with any microscope from any manufacturer. Of course, the microscope must be compatible, i.e. support RMS objectives, TL=160 mm, FL=45 mm.

Description of this part for the microscope.

eyepieces can be used with microscopes from any manufacturer, as long as the microscope supports eyepieces with a mounting diameter of 23.2 mm.

Description of this part for the microscope.

microscopes from any manufacturer, as long as the microscope supports eyepieces with a mounting diameter of 23.2 mm.

Description of this part for the microscope.

3x
Model: Semiplan 6.3/0.16 160/-
Color correction: Semi-Planachromat
Numerical aperture: 0.16
Tube length: 160 mm
Focal length: 45 mm
Cover glass: no
Immersion: no
Thread: RMS

This is one of the most commonly used microscope objectives for macro photography and focus stacking.

Important - this objective IS SUITABLE for taking images using the "direct-projection" technique, as it does not require plan-compensating optics.

It can be used with any microscope from any manufacturer. Of course, the microscope must be compatible, meaning it supports RMS objectives, TL=160 mm, FL=45 mm.

Description of this part for the microscope.

Manufacturer: Lomo
Magnification: 20x
Model: 20 0.40 P
Numerical aperture: 0.40
Tube length: 160 mm
Focal length: 33 mm
Cover glass: yes, 0.17 mm
Thread: RMS + Pol adapter
Info: P = Polarization

This is an objective designed for transmitted light work with a cover glass.

The RMS thread objective is mounted on a dedicated slide-in adapter that allows it to be used in Lomo MIN series and Lomo Polam series microscopes. Of course, the objective can be unscrewed and another objective can be mounted in the adapter.

The objective itself, once removed from the adapter, is a standard objective that can be used in virtually any microscope with an RMS thread. It is a so-called "short objective" (focal length 33 mm). When a 12 mm adapter is added, the total length becomes 45 mm, allowing it to be used with newer microscopes from any manufacturer.

Lomo polarization objectives with RMS thread can be installed in an RMS-M27 adapter and then used in microscopes such as Lomo Biolam M, Lomo Biolam I, Lomo Lumam, or Lomo ML-2. I have also frequently seen such objectives used in Zeiss Axio series microscopes with M27 thread.

The Pol adapter also allows precise centering of the objective relative to the entire optical path - this requires tools (keys / tools).

Info: I have repeatedly seen information stating that this RMS-Pol adapter is compatible with Leitz Wetzlar polarization microscopes - however, I have not personally verified this.

In summary, this objective is dedicated to Lomo MIN-1, MIN-4, MIN-5, MIN-6, MIN-8, Lomo Polam R-113, Polam L-211, Polam L-213, Polam R-311, Polam LMPR microscopes - but it is practically compatible with any microscope from any manufacturer that supports 160 mm objectives.

Description of this part for the microscope.

Note - this model of microscope objective is very often used for macro photography and focus stacking. More information on this can be found at the end of this description.

Manufacturer: Lomo
Magnification: 9x
Model: PLANACHR 9x0.20 P
Numerical aperture: 0.20
Optical correction: planachromatic (flat field)
Tube length: 160 mm
Focal length: 33 mm
Cover glass: optional, can be used but is not required
Thread: RMS + Pol adapter
Info: P = Polarization

The RMS thread objective is mounted on a dedicated slide-in adapter that allows it to be used in Lomo MIN series and Lomo Polam series microscopes. Of course, the objective can be unscrewed and another objective can be mounted into the adapter.

The objective itself, once removed from the adapter, is a standard objective that can be used in virtually any microscope with an RMS thread. It is a so-called "short objective" (focal length 33 mm). When a 12 mm adapter is added, the total length becomes 45 mm, allowing it to be used with newer microscopes from any manufacturer.

Lomo polarization objectives with RMS thread can be installed in an RMS-M27 adapter and then used in microscopes such as Lomo Biolam M, Lomo Biolam I, Lomo Lumam, or Lomo ML-2. I have also frequently seen such objectives used in Zeiss Axio series microscopes with M27 thread.

The Pol adapter also allows precise centering of the objective relative to the entire optical path - this requires tools (keys / tools).

Info: I have repeatedly seen information stating that this RMS-Pol adapter is compatible with Leitz Wetzlar polarization microscopes - however, I have not personally verified this.

In summary, this objective is dedicated to Lomo MIN-1, MIN-4, MIN-5, MIN-6, MIN-8, Lomo Polam R-113, Polam L-211, Polam L-213, Polam R-311, Polam LMPR microscopes - but it is practically compatible with any microscope from any manufacturer that supports 160 mm objectives.

Additionally, this objective is very often used for macro photography even without a microscope. This objective does not require a cover glass and works in the "direct-projection" technique, meaning it can be connected directly to a camera without additional optics. This allows achieving a very high magnification scale and great depth of field across the entire image when using focus stacking. The fact that this is a polarization objective (i.e. stress-free optics without internal glass tensions) further improves image quality at high magnifications.

Description of this part for the microscope.

Note - this model of microscope objective is very often used for macro photography and focus stacking. More information on this can be found at the end of this description.

Manufacturer: Lomo
Magnification: 3,7x
Model: 3,7 0,11
Numerical aperture: 0.11
Tube length: 160 mm
Focal length: 33 mm
Cover glass: optional, can be used but is not required
Thread: RMS + Pol adapter
Info: P = Polarization

The RMS thread objective is mounted on a dedicated slide-in adapter that allows it to be used in Lomo MIN series and Lomo Polam series microscopes. Of course, the objective can be unscrewed and another objective can be mounted into the adapter.

The objective itself, once removed from the adapter, is a standard objective that can be used in virtually any microscope with an RMS thread. It is a so-called "short objective" (focal length 33 mm). When a 12 mm adapter is added, the total length becomes 45 mm, allowing it to be used with newer microscopes from any manufacturer.

Lomo polarization objectives with RMS thread can be installed in an RMS-M27 adapter and then used in microscopes such as Lomo Biolam M, Lomo Biolam I, Lomo Lumam, or Lomo ML-2. I have also frequently seen such objectives used in Zeiss Axio series microscopes with M27 thread.

The Pol adapter also allows precise centering of the objective relative to the entire optical path - this requires tools (keys / tools).

Info: I have repeatedly seen information stating that this RMS-Pol adapter is compatible with Leitz Wetzlar polarization microscopes - however, I have not personally verified this.

In summary, this objective is dedicated to Lomo MIN-1, MIN-4, MIN-5, MIN-6, MIN-8, Lomo Polam R-113, Polam L-211, Polam L-213, Polam R-311, Polam LMPR microscopes - but it is practically compatible with any microscope from any manufacturer that supports 160 mm objectives.

Additionally, this objective is very often used for macro photography even without a microscope. This objective does not require a cover glass and works in the "direct-projection" technique, meaning it can be connected directly to a camera without additional optics. This allows achieving a very high magnification scale and great depth of field across the entire image when using focus stacking. The fact that this is a polarization objective (i.e. stress-free optics without internal glass tensions) further improves image quality at high magnifications. Info: https://www.closeuphotography.com/lomo-3-7x-objective/2017/12/24/lomo-37x-011-microscope-objective

Description of this part for the microscope.

for taking microscope photographs or recording microscope videos. This is a gray photographic adapter.

Basic technical parameters:
- mounting connection to microscope stand: 41 mm dovetail
- output connection type on the photo port: 49 mm internal thread
- connection for binocular head mounting: Jena standard 41 mm dovetail
- magnification directed to the binocular head: 1.6x
- magnification on the photo output: none (that is 1.0x)

Because this photo head has a 49 mm threaded output, virtually any device, camera, adapter, tube or other accessory can be connected after using an appropriate 49 mm thread adapter. Direct projection technique may be used (because there is no additional optics inside), or any projection eyepieces may be installed in the optical path.

This photographic attachment is intended mainly (but not exclusively) for the following microscope models:
- Carl Zeiss Jena Eduval
- Carl Zeiss Jena Laboval
- Carl Zeiss Jena Ergaval
- Carl Zeiss Jena Amplival
- Carl Zeiss Jena Vertival
- Carl Zeiss Jena Epival
- Carl Zeiss Jena Metaval
- Carl Zeiss Jena Telaval
- Carl Zeiss Jena Pictoval
- Carl Zeiss Jena Epignost

The list above contains Mikroval series microscopes with gray housings - just like the tube shown and offered here. However, it also works with older black Carl Zeiss Jena microscopes:
- Carl Zeiss Jena L-Stativ (models Lg, Lu and Lr)
- Carl Zeiss Jena Lumipan
- Carl Zeiss Jena Ng
- Carl Zeiss Jena Nf (and NfPk)

Please note that microscopes from other manufacturers also use the 41 / 41.5 mm dovetail connection standard. Because of this, this photographic head can also be used with microscopes made by:
- Lomo (for example Lomo Biolam)
- Meopta
- IOR
- selected Reichert microscopes (for example Reichert Neozet)
- Will Wetzlar
- Hund Wetzlar
- Leitz Wetzlar

This Carl Zeiss Jena tube is also very often used with microscopes having a larger head mounting diameter. In such cases users apply additional spacer or thickening rings. Because of this, this photo adapter also fits microscopes from manufacturers such as:
- PZO (for example the PZO Biolar model with 43 mm connection)
- Olympus (for example the Olympus CH model with 42 mm connection)

The element that allows a photographic camera or imaging camera to be connected to Carl Zeiss Jena Mikroval series microscopes may be referred to by different names. Commonly used names include:
- trino head
- trinocular head
- photo head
- photographic head
- photo attachment
- photographic attachment
- photo adapter
- photographic adapter
- photo tube
- photographic tube
- phototube
- photographic angle tube
- and various combinations of the words head, attachment, adapter or tube with terms such as photo, photographic, for camera and similar

Description of this part for the microscope.

Poland
- Model: 5/0.15 160/-
- Alternative model marking: 5/0.15 160/0
- Magnification: 5x
- Numerical aperture N.A.: 0.15
- Correction: achromatic
- Focal length FL: 33 mm
- Tube length: 160 mm
- Cover glass: not required, but may be used
- Immersion: no (dry objective)
- Thread: RMS

This model has a metal housing with no lens at its end. Additionally, this housing can be unscrewed without affecting the operation of the objective - resulting in a relatively large working distance of more than 20 mm.

Three features:
- large working distance exceeding 20 mm
- exceptionally high numerical aperture N.A. of 0.15
- achromatic correction allowing direct projection use
make this relatively uncommon model very frequently chosen for macro photography using a microscope objective connected to a camera (focus stacking).

The fact that this objective has FL = 33 mm allows it to be used with microscopes from other manufacturers, including:
- Lomo Biolam
- Lomo MBB-1A
- Carl Zeiss Jena LG / Lu / Lumipan
- Carl Zeiss Jena Nf
- Olympus CH

Note! Please note that the FL = 33 mm focal length means that this objective DOES NOT FIT the following microscopes:
- PZO Biolar
- PZO Studar

Early versions of this objective (with low serial numbers) have the engraved marking 5/0.15 160/0 suggesting that the objective is intended for use without a cover glass. This is true, but more precisely this objective can work both without a cover glass and with one. In newer versions the engraving already reads 5/0.15 160/- where this "minus" already means "indifferent".

Description of this part for the microscope.

mm
Cover Glass: 0.17 mm
Immersion: no
Thread: RMS

It can be used with any microscope from any manufacturer. The microscope must be compatible, meaning it supports RMS objectives, TL = 160 mm, FL = 33 mm.

After installing the 12 mm adapter, this objective lens will have FL = 45 mm.

The fact that this objective has FL = 33 mm allows it to be used with microscopes from other manufacturers, including:
- Lomo Biolam
- Lomo MBB-1A
- PZO MB-10
- Olympus CH

Description of this part for the microscope.

Length: 33 mm
Cover Glass: 0.17 mm
Immersion: no
Thread: RMS

It can be used with any microscope from any manufacturer. The microscope must be compatible, meaning it supports RMS objectives, TL = 160 mm, FL = 33 mm.

After installing the 12 mm adapter, this objective lens will have FL = 45 mm.

The fact that this objective has FL = 33 mm allows it to be used with microscopes from other manufacturers, including:
- Carl Zeiss Jena Lg
- Carl Zeiss Jena Lumipan
- Lomo Biolam
- Lomo MBB-1A
- PZO MB-10
- Nikon S
- Olympus KHC

Description of this part for the microscope.

35 160/0.17
Manufacturer part number: 46 05 10-9906 (alternative notation: 460510 - 9906)
Color correction: Planachromat
Numerical aperture N.A.: 0.35
Tube length: 160 mm
Focal length: 45 mm
Cover glass: yes, 0.17 mm thickness
Immersion: no (dry objective)
Thread: RMS

Description of this part for the microscope.

65 160/0.17
Manufacturer part number: 46 07 10-9904 (alternative notation: 460710 - 9904)
Color correction: Planachromat
Numerical aperture N.A.: 0.65
Tube length: 160 mm
Focal length: 45 mm
Cover glass: yes, 0.17 mm thickness
Immersion: no (dry objective)
Thread: RMS

Description of this part for the microscope.

17
Magnification: 40x
Correction: Achromat
Numerical aperture: 0.65
Tube length: 160 mm
Focal length: 33 mm
Cover glass: yes, 0.17 mm thickness
Immersion: no (dry objective)
Thread: RMS

It can be used with any microscope from any manufacturer. Of course, the microscope must be compatible, meaning it must support RMS objectives with TL=160 mm and FL=33 mm. After installing a 12 mm adapter, this objective can of course also be used in microscopes designed for FL=45 mm.

The fact that this objective has FL = 33 mm (meaning it is a so-called short objective) allows it to be used with microscopes from other manufacturers, including:
- Carl Zeiss Jena Lg
- Carl Zeiss Jena Lumipan
- Lomo Biolam
- Lomo MBB-1A
- PZO MB-10
- Nikon S
- Olympus KHC

Description of this part for the microscope.

although incorrectly, referred to as "Lomo")
Model: 20 0,40
Magnification: 20x
Correction: Achromat
Numerical aperture: 0.40
Tube length: 160 mm
Focal length: 33 mm
Cover glass: 0.17 mm
Immersion: no
Thread: RMS
Serial number: 01234

It can be used with any microscope from any manufacturer. Of course, the microscope must be compatible, i.e. support RMS objectives, TL=160 mm and FL=33 mm.

After installing a 12 mm adapter, this combination of adapter and objective will function as an objective with FL=45 mm.

This objective is attractive mainly to collectors because:
- there are very few microscope objectives with the LZOS logo on the secondary market
- the objective has a so-called "golden serial number": 01234

Description of this part for the microscope.

Wetzlar
Model: 160/0,17 EF 100/1.25 OEL PHACO 3
Magnification: 100x
Correction: semi-plan achromat
Numerical aperture: 1.25
Tube length: 160 mm
Focal length: 45 mm
Cover glass: yes, 0.17 mm
Immersion: yes, oil
Thread: RMS
Specimen protection: yes - spring-loaded
Info: PHACO 3 = phase contrast

Some important information about this objective:
- the designation "EF" means "Extended Field" - this is a semi-plan achromatic objective with an 18 mm field of view, which should be taken into account when selecting suitable eyepieces
- the semi-plan achromatic correction is "almost flat" - it is no longer an achromat, but not yet a plan achromat
- this objective can be used in brightfield, but phase contrast operation requires a compatible phase contrast condenser or a slide (slider) with a phase ring diaphragm
- the designation PHACO 3 means that for phase contrast operation a phase contrast condenser set to ring "3" should be used (the equivalent setting used by Zeiss is called "Ph3")
- this objective can be used with microscopes designed for objectives with a 170 mm tube length, but in such a case the optical path must be extended by 10 mm - most commonly by pulling the eyepieces out of the tubes by 10 mm

This objective can be used with any microscope from any manufacturer together with a compatible phase contrast condenser. Naturally, the microscope must be compatible, i.e. it must support RMS objectives, TL=160 mm and FL=45 mm - or have adapters that make such adaptation possible (for example thread adapters).

Description of this part for the microscope.

ameters:
Manufacturer: Lomo
Model: PLAN 25x0.50 P
Original designation: ЛОМО ПЛАН 25х0,50 П
Magnification: 25x
Numerical aperture N.A.: 0.50
Tube length: 160 mm
Cover glass: yes, 0.17 mm thickness
Immersion oil: no - this is a dry objective
Focal length: 45 mm
Thread type: RMS
Additional designation: P (Cyrillic: П) indicates that this objective is designed for use with polarized light
Special features: built-in iris diaphragm
Year of manufacture: 1976

This objective has a standard RMS thread and TL=160 mm, which means it can be used with the vast majority of microscopes from virtually any manufacturer, including Olympus, Zeiss, Nikon, PZO, Carl Zeiss Jena, Reichert, Wild and many others.

The built-in iris diaphragm allows, among other things, precise centering of the objective, because in a polarizing microscope the following diaphragms may operate simultaneously:
- field diaphragm in the illuminator
- aperture diaphragm (or two aperture diaphragms) in the condenser
- iris diaphragm in the microscope objective
- iris diaphragm in the eyepiece head
and accurate centering of all of them relative to each other is very important in polarized-light observations.

Objectives marked "P" / "Pol" feature a special construction in which the lenses are mounted and cemented in a way that prevents mechanical stress. They can be used both for conventional brightfield microscopy and for observations in polarized light.

Description of this part for the microscope.

3x
Model: Semiplan 6.3/0.16 160/-
Serial number: 039 562 (039562)
Color correction: Semi-Planachromat
Numerical aperture: 0.16
Tube length: 160 mm
Focal length: 45 mm
Cover glass: no
Immersion: no
Thread: RMS

This is one of the most commonly used microscope objectives for macro photography and focus stacking.

Important - this objective IS SUITABLE for taking images using the "direct-projection" technique, as it does not require plan-compensating optics.

It can be used with any microscope from any manufacturer. Of course, the microscope must be compatible, meaning it supports RMS objectives, TL=160 mm, FL=45 mm.

Description of this part for the microscope.

for inverted and upright biological microscopes, made in Japan.

Basic objective parameters:
Manufacturer: Nikon, Japan
Model: Ph3 40 DL 0.55 LWD 160/0-2
Magnification: 40x
Numerical aperture (N.A.): 0.55
Optical correction: achromat
Phase contrast: Ph3 DL - Ph3 indicates the size of the phase ring (requires a matching Ph3 annulus in the condenser), DL (Dark Low) means dark, i.e. positive phase contrast - the object appears darker than the background
Working distance (WD): ~2.1 - 2.5 mm - LWD (Long Working Distance)
Correction collar: compensates for cover glass or vessel bottom thickness in the range 0 - 2.0 mm
Cover glass: the objective can be used without a cover glass, with a standard cover glass approx. 0.17 mm thick, and also with specimens observed through the bottom of a Petri dish up to 2.0 mm thick
Immersion: no (dry objective)
Tube length: 160 mm
Parfocal length: 45 mm
Thread: RMS
Optics: CF (Nikon CF-160) - requires no compensating eyepiece and allows photography by direct projection

This objective is suitable for:
- inverted microscopes - observation through the bottom of a Petri dish or cell culture flask
- upright biological microscopes - observation of specimens with or without a cover glass

Phase contrast work requires a compatible phase contrast condenser or a slider with a phase contrast annulus.

Commonly used, inexpensive cover glasses are 0.17 mm thick mainly in theory. Even the standard for class #1.5 allows a range of 0.16 - 0.19 mm, and in cheap Chinese cover glasses you will find in practice pieces from approx. 0.13 to 0.25 mm, often not matching the thickness stated on the box. Only #1.5H (high precision) cover glasses have a guaranteed thickness of 0.170 mm with a tolerance of +/- 0.005 mm. Variation in cover glass thickness clearly degrades image contrast and resolution (spherical aberration). The correction collar fitted to the objective offered here lets you adjust the optics to the combined thickness of the cover glass and the medium layer beneath it. This compensates for most of the variation found in practice.

In addition, the correction collar of this objective can be set to "0" for work without a cover glass, or to large values (up to 2.0 mm) to adapt the optics to observation through a microscope slide (0.8 - 1.2 mm), a glass Petri dish (usually 1.5 - 2.0 mm - thicker dishes are outside the collar range) or a plastic Petri dish (usually approx. 1 mm, in the range 0.8 - 1.2 mm).

This objective fits practically any microscope from any manufacturer, provided it has an RMS thread and uses objectives for 160 mm tube length with a parfocal length of 33, 37 or 45 mm. Some example microscopes are listed in the "compatibility" section, but the list is not complete.

Description of this part for the microscope.

20x
Correction: Fluotar (other names: Fluor and fluorite)
Numerical aperture N.A.: 0.60
Tube length: 160 mm
Parfocal length: 33 mm
Cover glass: yes, 0.17 mm thick
Immersion: no (dry objective)
Thread: RMS

The objective comes in its original protective case marked "Wild Heerbrugg Switzerland".

Fluotar is the name Wild Heerbrugg used for its fluorite objectives, i.e. semi-apochromats - their lenses contain fluorite or glass with similar optical properties. A standard achromat corrects chromatic errors for only two colors (red and blue), so colored fringes may appear along the edges of objects. An apochromat corrects for three colors (red, green and blue) and represents the highest class of correction. The Fluotar sits between them - colored fringes are clearly smaller than with an achromat, although the correction is not as complete as with an apochromat. Fluorite also transmits ultraviolet light well, which is why objectives of this type were used in fluorescence microscopy.

This is a so-called "short barrel" objective with a parfocal length of 33 mm. It is very common to mount this objective on a 12 mm extension, which allows it to be used in microscopes that require 45 mm parfocal objectives. Professional adapters with protective optics, metal adapters and popular DIY 3D-printed adapters all work well for this.

This objective fits practically any microscope from any manufacturer, as long as it has an RMS thread and accepts objectives with TL=160 mm and a parfocal length of 33 mm (or 37 / 45 mm with a suitable adapter). Several example microscopes are listed in the "compatibility" section, but the list is not complete.