Home page of the microscope service parts, repair and spare parts online catalog - Sermik-Oplatek Mikroskopservice Dresden f.n.n.Z

Description of this part for the microscope.

mm
Focal Length: 45 mm
Cover Glass: No
Immersion: No
Thread: RMS

It can be used with any microscope from any manufacturer. Of course, the microscope must be compatible, meaning it supports RMS objectives, TL=160 mm, FL=45 mm.

Description of this part for the microscope.

Length: 45 mm
Cover Glass: yes, 0,17 mm
Immersion: yes, oil
Thread: RMS

It can be used with any microscope from any manufacturer. Of course, the microscope must be compatible, meaning it supports RMS objectives, TL=160 mm, FL=45 mm.

Description of this part for the microscope.

Length: 45 mm
Cover Glass: yes, 0,17 mm
Immersion: yes, oil
Thread: RMS

This is an oil-immersion objective with a high numerical aperture. It is recommended for working with fluorescent light or for those whose primary magnification is around 40x, 45x, 50x, 55x, 60x, or 63x and who want to achieve the highest possible quality and detail visibility.

It can be used with any microscope from any manufacturer. Of course, the microscope must be compatible, meaning it supports RMS objectives, TL=160 mm, FL=45 mm.

Description of this part for the microscope.

compatible with microscopes designed for a 45 mm parfocal standard It is mounted between the objective and the nosepiece/revolver so that optical focus is maintained despite the additional mechanical extension.

Leitz PLEZY differs from extension adapters available on the market in that it features internal anti-reflective optics, ensuring that despite the mechanical extension of the focal length the image quality is not degraded.

Description of this part for the microscope.

immersion operation and with a phase contrast ring.

Manufacturer: Lomo
Model: APO-WI-F 70x1,23
Magnification: 70x
Numerical aperture: 1,23
Correction: Apochromat
Tube length: 160 mm
Focal length: 33 mm
Immersion: yes - water
Cover glass: yes - thickness from 0.10 to 0.20 mm
Markings: APO = Apochromat, WI = water immersion, F = phase contrast
Thread: RMS
Year of production: 1972

Note: to work in phase contrast technology you need a Lomo KF-4 condenser (you can see it in the database under number 00653).

This is a professional microscope objective with a very wide range of applications. The main features that make this objective exceptional:
- water immersion is preferred by many users over oil immersion
- very high numerical aperture, allowing the objective to produce a high resolution image
- cover glass thickness correction ring

Standard biological objectives are designed to achieve the best image quality when the specimen is located under a cover glass with a thickness of 0.17 mm (hence the marking on objectives such as 160/0.17). Unfortunately inexpensive cover glasses often do not undergo strict production control and a single package may contain cover glasses with thicknesses of 0.14 mm or 0.19 mm, which reduces observation sharpness.
Thanks to the built-in cover glass thickness correction ring (abbreviated CGTCR, CGCC or CGCR) the objective can be adjusted for every specimen so that it operates optimally. This works even when the actual thickness of the cover glass is unknown, because the effect is simply visible and the adjustment can be done "by eye".

Although this objective is a so-called "short" objective, meaning with a parfocal length of 33 mm, after adding a 12 mm adapter (you can see it in the database under number 99140) it can be used with newer microscopes designed for objectives with a parfocal length of 45 mm.

The Lomo company produced adapters specifically for these objectives (converters) from RMS thread to M27 thread, which makes them a very attractive option for installation in modern Zeiss Axio series microscopes.

Description of this part for the microscope.

160 mm
Focal Length: 45 mm
Cover Glass: no
Immersion: no
Thread: RMS
Info: Phv = phase contrast variable

It can be used with any microscope from any manufacturer, along with a compatible Phv phase contrast condenser. The microscope must be compatible, meaning it supports RMS objectives, TL = 160 mm, FL = 45 mm.

Description of this part for the microscope.

08 160/-
Color correction: Planachromat
Numerical aperture: 0.08
Tube length: 160 mm
Focal length: 45 mm
Cover glass: no
Immersion: no
Thread: RMS

This lens provides an excellent, wide image, and because it doesn't require a cover glass, it's often used for macro photography and stacking.

Important - this lens is not suitable for taking photos using the "direct-projection" technique, as it requires plan-compensation optics.

A microscope objective with such low magnification is used, among others, in the following fields:
- tissue overview examination in pathomorphology
- observation of insects
- observation of minerals
- observation of inclusions in amber
- handwriting analysis
- observation of micromollusks
- and many others

Description of this part for the microscope.

160 mm
Focal Length: 45 mm
Cover Glass: yes, 0,17 mm
Immersion: no
Thread: RMS
Info: Phv = phase contrast variable

It can be used with any microscope from any manufacturer, along with a compatible Phv phase contrast condenser. The microscope must be compatible, meaning it supports RMS objectives, TL = 160 mm, FL = 45 mm.

Description of this part for the microscope.

10 160/-
Color correction: Planachromat
Numerical aperture: 0.10
Tube length: 160 mm
Focal length: 45 mm
Cover glass: no
Immersion: no
Thread: RMS

This lens provides an excellent, wide image, and because it doesn't require a cover glass, it's often used for macro photography and stacking.

Important - this lens is not suitable for taking photos using the "direct-projection" technique, as it requires plan-compensation optics.

A microscope objective with such low magnification is used, among others, in the following fields:
- tissue overview examination in pathomorphology
- observation of insects
- observation of minerals
- observation of inclusions in amber
- handwriting analysis
- observation of micromollusks
- and many others

It can be used with any microscope from any manufacturer. Of course, the microscope must be compatible, i.e. support RMS objectives, TL=160 mm, FL=45 mm.

Description of this part for the microscope.

Color correction: Planachromat
Numerical aperture: 0.25
Tube length: 160 mm
Focal length: 45 mm
Cover glass: no
Immersion: no
Thread: RMS

It can be used with any microscope from any manufacturer. Of course, the microscope must be compatible, i.e. support RMS objectives, TL=160 mm, FL=45 mm.

Description of this part for the microscope.

17
Color correction: Planachromat
Numerical aperture: 0.32
Tube length: 160 mm
Focal length: 45 mm
Cover glass: yes, 0,17 mm
Immersion: no
Thread: RMS

It can be used with any microscope from any manufacturer. Of course, the microscope must be compatible, i.e. support RMS objectives, TL=160 mm, FL=45 mm.

Description of this part for the microscope.

17
Color correction: Planachromat
Numerical aperture: 0.65
Tube length: 160 mm
Focal length: 45 mm
Cover glass: yes, 0,17 mm
Immersion: no
Thread: RMS

It can be used with any microscope from any manufacturer. Of course, the microscope must be compatible, i.e. support RMS objectives, TL=160 mm, FL=45 mm.

Description of this part for the microscope.

17
Color correction: Planachromat
Numerical aperture: 1,25
Tube length: 160 mm
Focal length: 45 mm
Cover glass: yes, 0,17 mm
Immersion: yes, oil
Thread: RMS
Info: HI = Homogene Immersion, also oil immersion

It can be used with any microscope from any manufacturer. Of course, the microscope must be compatible, i.e. support RMS objectives, TL=160 mm, FL=45 mm.

Description of this part for the microscope.

3x
Model: Semiplan 6.3/0.16 160/-
Color correction: Semi-Planachromat
Numerical aperture: 0.16
Tube length: 160 mm
Focal length: 45 mm
Cover glass: no
Immersion: no
Thread: RMS

This is one of the most commonly used microscope objectives for macro photography and focus stacking.

Important - this objective IS SUITABLE for taking images using the "direct-projection" technique, as it does not require plan-compensating optics.

It can be used with any microscope from any manufacturer. Of course, the microscope must be compatible, meaning it supports RMS objectives, TL=160 mm, FL=45 mm.

Description of this part for the microscope.

Manufacturer: Lomo
Magnification: 20x
Model: 20 0.40 P
Numerical aperture: 0.40
Tube length: 160 mm
Focal length: 33 mm
Cover glass: yes, 0.17 mm
Thread: RMS + Pol adapter
Info: P = Polarization

This is an objective designed for transmitted light work with a cover glass.

The RMS thread objective is mounted on a dedicated slide-in adapter that allows it to be used in Lomo MIN series and Lomo Polam series microscopes. Of course, the objective can be unscrewed and another objective can be mounted in the adapter.

The objective itself, once removed from the adapter, is a standard objective that can be used in virtually any microscope with an RMS thread. It is a so-called "short objective" (focal length 33 mm). When a 12 mm adapter is added, the total length becomes 45 mm, allowing it to be used with newer microscopes from any manufacturer.

Lomo polarization objectives with RMS thread can be installed in an RMS-M27 adapter and then used in microscopes such as Lomo Biolam M, Lomo Biolam I, Lomo Lumam, or Lomo ML-2. I have also frequently seen such objectives used in Zeiss Axio series microscopes with M27 thread.

The Pol adapter also allows precise centering of the objective relative to the entire optical path - this requires tools (keys / tools).

Info: I have repeatedly seen information stating that this RMS-Pol adapter is compatible with Leitz Wetzlar polarization microscopes - however, I have not personally verified this.

In summary, this objective is dedicated to Lomo MIN-1, MIN-4, MIN-5, MIN-6, MIN-8, Lomo Polam R-113, Polam L-211, Polam L-213, Polam R-311, Polam LMPR microscopes - but it is practically compatible with any microscope from any manufacturer that supports 160 mm objectives.

Description of this part for the microscope.

Note - this model of microscope objective is very often used for macro photography and focus stacking. More information on this can be found at the end of this description.

Manufacturer: Lomo
Magnification: 9x
Model: PLANACHR 9x0.20 P
Numerical aperture: 0.20
Optical correction: planachromatic (flat field)
Tube length: 160 mm
Focal length: 33 mm
Cover glass: optional, can be used but is not required
Thread: RMS + Pol adapter
Info: P = Polarization

The RMS thread objective is mounted on a dedicated slide-in adapter that allows it to be used in Lomo MIN series and Lomo Polam series microscopes. Of course, the objective can be unscrewed and another objective can be mounted into the adapter.

The objective itself, once removed from the adapter, is a standard objective that can be used in virtually any microscope with an RMS thread. It is a so-called "short objective" (focal length 33 mm). When a 12 mm adapter is added, the total length becomes 45 mm, allowing it to be used with newer microscopes from any manufacturer.

Lomo polarization objectives with RMS thread can be installed in an RMS-M27 adapter and then used in microscopes such as Lomo Biolam M, Lomo Biolam I, Lomo Lumam, or Lomo ML-2. I have also frequently seen such objectives used in Zeiss Axio series microscopes with M27 thread.

The Pol adapter also allows precise centering of the objective relative to the entire optical path - this requires tools (keys / tools).

Info: I have repeatedly seen information stating that this RMS-Pol adapter is compatible with Leitz Wetzlar polarization microscopes - however, I have not personally verified this.

In summary, this objective is dedicated to Lomo MIN-1, MIN-4, MIN-5, MIN-6, MIN-8, Lomo Polam R-113, Polam L-211, Polam L-213, Polam R-311, Polam LMPR microscopes - but it is practically compatible with any microscope from any manufacturer that supports 160 mm objectives.

Additionally, this objective is very often used for macro photography even without a microscope. This objective does not require a cover glass and works in the "direct-projection" technique, meaning it can be connected directly to a camera without additional optics. This allows achieving a very high magnification scale and great depth of field across the entire image when using focus stacking. The fact that this is a polarization objective (i.e. stress-free optics without internal glass tensions) further improves image quality at high magnifications.

Description of this part for the microscope.

Note - this model of microscope objective is very often used for macro photography and focus stacking. More information on this can be found at the end of this description.

Manufacturer: Lomo
Magnification: 3,7x
Model: 3,7 0,11
Numerical aperture: 0.11
Tube length: 160 mm
Focal length: 33 mm
Cover glass: optional, can be used but is not required
Thread: RMS + Pol adapter
Info: P = Polarization

The RMS thread objective is mounted on a dedicated slide-in adapter that allows it to be used in Lomo MIN series and Lomo Polam series microscopes. Of course, the objective can be unscrewed and another objective can be mounted into the adapter.

The objective itself, once removed from the adapter, is a standard objective that can be used in virtually any microscope with an RMS thread. It is a so-called "short objective" (focal length 33 mm). When a 12 mm adapter is added, the total length becomes 45 mm, allowing it to be used with newer microscopes from any manufacturer.

Lomo polarization objectives with RMS thread can be installed in an RMS-M27 adapter and then used in microscopes such as Lomo Biolam M, Lomo Biolam I, Lomo Lumam, or Lomo ML-2. I have also frequently seen such objectives used in Zeiss Axio series microscopes with M27 thread.

The Pol adapter also allows precise centering of the objective relative to the entire optical path - this requires tools (keys / tools).

Info: I have repeatedly seen information stating that this RMS-Pol adapter is compatible with Leitz Wetzlar polarization microscopes - however, I have not personally verified this.

In summary, this objective is dedicated to Lomo MIN-1, MIN-4, MIN-5, MIN-6, MIN-8, Lomo Polam R-113, Polam L-211, Polam L-213, Polam R-311, Polam LMPR microscopes - but it is practically compatible with any microscope from any manufacturer that supports 160 mm objectives.

Additionally, this objective is very often used for macro photography even without a microscope. This objective does not require a cover glass and works in the "direct-projection" technique, meaning it can be connected directly to a camera without additional optics. This allows achieving a very high magnification scale and great depth of field across the entire image when using focus stacking. The fact that this is a polarization objective (i.e. stress-free optics without internal glass tensions) further improves image quality at high magnifications. Info: https://www.closeuphotography.com/lomo-3-7x-objective/2017/12/24/lomo-37x-011-microscope-objective

Description of this part for the microscope.

Length: 45 mm
Cover Glass: yes, 0,17 mm
Immersion: no
Thread: RMS
Info: Phv = phase contrast variable

It can be used with any microscope from any manufacturer, along with a compatible Phv phase contrast condenser. The microscope must be compatible, meaning it supports RMS objectives, TL = 160 mm, FL = 45 mm.

Description of this part for the microscope.

microscope objective
- Manufacturer: Carl Zeiss Jena
- Designation: Planachromat 25x/0,50 oo/0,17
- Magnification: 25x
- Numerical aperture N.A.: 0,50
- Optical correction: infinity-corrected tube length
- Optical correction class: planachromat
- Cover glass: yes, 0.17 mm
- Thread: M19

Some facts about Carl Zeiss Jena objectives with M19 thread (CZJ M19) and their compatibility with various microscopes from this manufacturer:
- these objectives are most commonly used for technical applications rather than biological applications, therefore most objectives available on the secondary market are intended for use without a cover glass (designation oo/0)
- CZJ M19 objectives intended for biological applications (designation oo/0,17) are most commonly used on Carl Zeiss Jena NU / NU2 microscopes
- in Carl Zeiss Jena Nf and Lumipan microscopes, CZJ M19 objectives are used together with an EPI illuminator
- CZJ M19 objectives can be used in Carl Zeiss Jena Polmi-A microscopes, but they provide a different magnification than the nominal value
- Carl Zeiss Jena Amplival / Amplival Pol / Amplival Interphako / Peraval microscopes feature interchangeable nosepieces, and a dedicated nosepiece for M19-thread objectives was also produced for these microscopes
- Carl Zeiss Jena Metaval and Neophot microscopes use newer versions of CZJ M19 objectives (with a coarse-knurled edge and shiny housing), although many users still experiment with older M19 objectives on these microscopes
- Carl Zeiss Jena Epignost and BK 70x50 microscopes were produced in various versions with completely different configurations - and some of them operate with CZJ M19 objectives
- Carl Zeiss Jena Vertival / Epival / Nf / BK 70x50 microscopes often allow the use of CZJ M19 objectives in EPI dark-field mode
- on the Carl Zeiss Jena Epityp-2 inverted metallurgical microscope, many users employ biological objectives designed for use with a cover glass, placing the microscope slide "upside down", with the cover glass facing downward toward the objective.

Description of this part for the microscope.

35 160/0.17
Manufacturer part number: 46 05 10-9906 (alternative notation: 460510 - 9906)
Color correction: Planachromat
Numerical aperture N.A.: 0.35
Tube length: 160 mm
Focal length: 45 mm
Cover glass: yes, 0.17 mm thickness
Immersion: no (dry objective)
Thread: RMS

Description of this part for the microscope.

65 160/0.17
Manufacturer part number: 46 07 10-9904 (alternative notation: 460710 - 9904)
Color correction: Planachromat
Numerical aperture N.A.: 0.65
Tube length: 160 mm
Focal length: 45 mm
Cover glass: yes, 0.17 mm thickness
Immersion: no (dry objective)
Thread: RMS

Description of this part for the microscope.

yes, 0.17 mm
Thread: RMS
Tube length: 160 mm
Focal length: 45 mm
Immersion: yes, oil immersion
Thread: RMS

Only towards the end of its operations did Hertel & Reuss begin producing microscopes with objectives having a 45 mm parfocal length. These were the Hertel & Reuss Labopan and Superpan models. Previously, microscopes from this company used objectives with a 37 mm parfocal length.

This objective fits practically any microscope from any manufacturer, provided that it has an RMS thread and supports objectives with TL=160 mm. Several examples of such microscopes are listed in the "Compatibility" section, but this is not a complete list.

Description of this part for the microscope.

10
Cover glass: not required
Thread: RMS
Tube length: 160 mm
Focal length: 45 mm
Immersion: no (dry objective)
Thread: RMS

This objective can be used both:
- with biological microscopes using transmitted-light illumination
- with inverted microscopes
- with microscopes with reflected-light illumination
- for macro photography by directly connecting it to a camera

This objective fits practically any microscope from any manufacturer, provided that it has an RMS thread and supports objectives for TL=160 mm. Several examples of such microscopes are listed in the "compatibility" section, but this is not a complete list.

Several facts are worth emphasizing:
- many Hund Wetzlar microscope models are identical to models sold by Will Wetzlar due to their joint production. For example, the BX300 and Wilovert models even have the same name. Therefore, Hund Wetzlar microscope objectives can also be used with Will Wetzlar microscopes.
- the companies Eschenbach, Kremp Wetzlar, CBS Beck Kassel and Kosmos Stuttgart also used Will Wetzlar objectives, so Hund Wetzlar microscope objectives can also be used with microscopes from these manufacturers - but it is worth checking the parfocal length first (37 mm or 45 mm)
- objectives with magnifications below 16x (e.g. 4x / 5x or 10x) fit both classic biological microscopes, microscopes with reflected-light illuminators and inverted microscopes.

Description of this part for the microscope.

25
Cover glass: not required
Thread: RMS
Tube length: 160 mm
Focal length: 45 mm
Immersion: no (dry objective)
Thread: RMS

This objective can be used both:
- with biological microscopes using transmitted-light illumination
- with inverted microscopes
- with microscopes with reflected-light illumination
- for macro photography by directly connecting it to a camera

This objective fits practically any microscope from any manufacturer, provided that it has an RMS thread and supports objectives for TL=160 mm. Several examples of such microscopes are listed in the "compatibility" section, but this is not a complete list.

Several facts are worth emphasizing:
- many Hund Wetzlar microscope models are identical to models sold by Will Wetzlar due to their joint production. For example, the BX300 and Wilovert models even have the same name. Therefore, Hund Wetzlar microscope objectives can also be used with Will Wetzlar microscopes.
- the companies Eschenbach, Kremp Wetzlar, CBS Beck Kassel and Kosmos Stuttgart also used Will Wetzlar objectives, so Hund Wetzlar microscope objectives can also be used with microscopes from these manufacturers - but it is worth checking the parfocal length first (37 mm or 45 mm)
- objectives with magnifications below 16x (e.g. 4x / 5x or 10x) fit both classic biological microscopes, microscopes with reflected-light illuminators and inverted microscopes.

Description of this part for the microscope.

65
Cover glass: yes, 0,17 mm
Thread: RMS
Tube length: 160 mm
Focal length: 45 mm
Immersion: no (dry objective)
Thread: RMS

This objective fits practically any microscope from any manufacturer, provided that it has an RMS thread and supports objectives for TL=160 mm. Several examples of such microscopes are listed in the "compatibility" section, but this is not a complete list.

- many Hund Wetzlar microscope models are identical to models sold by Will Wetzlar due to their joint production. For example, the BX300 and Wilovert models even have the same name. Therefore, Hund Wetzlar microscope objectives can also be used with Will Wetzlar microscopes.
- the companies Eschenbach, Kremp Wetzlar, CBS Beck Kassel and Kosmos Stuttgart also used Will Wetzlar objectives, so Hund Wetzlar microscope objectives can also be used with microscopes from these manufacturers - but it is worth checking the parfocal length first (37 mm or 45 mm)

Description of this part for the microscope.

25
Cover glass: yes, 0.17 mm
Thread: RMS
Tube length: 160 mm
Focal length: 45 mm
Immersion: yes, oil immersion
Thread: RMS

This objective fits practically any microscope from any manufacturer, provided that it has an RMS thread and supports objectives for TL=160 mm. Several examples of such microscopes are listed in the "compatibility" section, but this is not a complete list.

- many Hund Wetzlar microscope models are identical to models sold by Will Wetzlar due to their joint production. For example, the BX300 and Wilovert models even have the same name. Therefore, Hund Wetzlar microscope objectives can also be used with Will Wetzlar microscopes.
- the companies Eschenbach, Kremp Wetzlar, CBS Beck Kassel and Kosmos Stuttgart also used Will Wetzlar objectives, so Hund Wetzlar microscope objectives can also be used with microscopes from these manufacturers - but it is worth checking the parfocal length first (37 mm or 45 mm)

Description of this part for the microscope.

3x
Model: Semiplan 6.3/0.16 160/-
Serial number: 039 562 (039562)
Color correction: Semi-Planachromat
Numerical aperture: 0.16
Tube length: 160 mm
Focal length: 45 mm
Cover glass: no
Immersion: no
Thread: RMS

This is one of the most commonly used microscope objectives for macro photography and focus stacking.

Important - this objective IS SUITABLE for taking images using the "direct-projection" technique, as it does not require plan-compensating optics.

It can be used with any microscope from any manufacturer. Of course, the microscope must be compatible, meaning it supports RMS objectives, TL=160 mm, FL=45 mm.

Description of this part for the microscope.

for inverted and upright biological microscopes, made in Japan.

Basic objective parameters:
Manufacturer: Nikon, Japan
Model: Ph3 40 DL 0.55 LWD 160/0-2
Magnification: 40x
Numerical aperture (N.A.): 0.55
Optical correction: achromat
Phase contrast: Ph3 DL - Ph3 indicates the size of the phase ring (requires a matching Ph3 annulus in the condenser), DL (Dark Low) means dark, i.e. positive phase contrast - the object appears darker than the background
Working distance (WD): ~2.1 - 2.5 mm - LWD (Long Working Distance)
Correction collar: compensates for cover glass or vessel bottom thickness in the range 0 - 2.0 mm
Cover glass: the objective can be used without a cover glass, with a standard cover glass approx. 0.17 mm thick, and also with specimens observed through the bottom of a Petri dish up to 2.0 mm thick
Immersion: no (dry objective)
Tube length: 160 mm
Parfocal length: 45 mm
Thread: RMS
Optics: CF (Nikon CF-160) - requires no compensating eyepiece and allows photography by direct projection

This objective is suitable for:
- inverted microscopes - observation through the bottom of a Petri dish or cell culture flask
- upright biological microscopes - observation of specimens with or without a cover glass

Phase contrast work requires a compatible phase contrast condenser or a slider with a phase contrast annulus.

Commonly used, inexpensive cover glasses are 0.17 mm thick mainly in theory. Even the standard for class #1.5 allows a range of 0.16 - 0.19 mm, and in cheap Chinese cover glasses you will find in practice pieces from approx. 0.13 to 0.25 mm, often not matching the thickness stated on the box. Only #1.5H (high precision) cover glasses have a guaranteed thickness of 0.170 mm with a tolerance of +/- 0.005 mm. Variation in cover glass thickness clearly degrades image contrast and resolution (spherical aberration). The correction collar fitted to the objective offered here lets you adjust the optics to the combined thickness of the cover glass and the medium layer beneath it. This compensates for most of the variation found in practice.

In addition, the correction collar of this objective can be set to "0" for work without a cover glass, or to large values (up to 2.0 mm) to adapt the optics to observation through a microscope slide (0.8 - 1.2 mm), a glass Petri dish (usually 1.5 - 2.0 mm - thicker dishes are outside the collar range) or a plastic Petri dish (usually approx. 1 mm, in the range 0.8 - 1.2 mm).

This objective fits practically any microscope from any manufacturer, provided it has an RMS thread and uses objectives for 160 mm tube length with a parfocal length of 33, 37 or 45 mm. Some example microscopes are listed in the "compatibility" section, but the list is not complete.

Description of this part for the microscope.

100x
Colour correction: achromatic
Model: E. 100 1.25 oil 160/0.17
Numerical aperture N.A.: 1.25
Cover glass: yes, 0.17 mm thick
Thread: RMS
Tube length: 160 mm
Parfocal length: 45 mm (long barrel objective)
Immersion: yes, oil
Operating mode: objectives marked "E." are already CF objectives (CFN to be precise) and need no additional correction

This objective needs no additional correction from a tube lens or from the eyepieces. That makes it possible to fit it to microscopes of other makes, and in photomicrography it works in direct projection mode (it throws the image straight onto the camera sensor, with no relay optics needed).

This is the so-called black series of objectives, named after the black plastic barrel. They were used mainly in the Nikon Alphaphot YS2 microscope. That was the period when manufacturers were experimenting with a new look for microscope parts, and exactly the same black series appeared for instance in the Leitz / Leica HM-LUX 3 microscope or in the Will Wetzlar V300 / V355 / V365 microscopes.

This objective fits practically any microscope of any make, provided it has an RMS thread and supports objectives with TL = 160 mm and parfocal lengths of 33 / 37 and 45 mm. A few such microscopes are listed in the "compatibility" section, but that list is not complete.

Description of this part for the microscope.

correction: achromatic
Model: E 40 0.65 160/0.17
Numerical aperture N.A.: 0.65
Cover glass: yes, 0.17 mm thick
Thread: RMS
Tube length: 160 mm
Parfocal length: 45 mm (long barrel objective)
Immersion: no
Operating mode: objectives marked "E." are already CF objectives (CFN to be precise) and need no additional correction

This objective needs no additional correction from a tube lens or from the eyepieces. That makes it possible to fit it to microscopes of other makes, and in photomicrography it works in direct projection mode (it throws the image straight onto the camera sensor, with no relay optics needed).

This is the so-called black series of objectives, named after the black plastic barrel. They were used mainly in the Nikon Alphaphot YS2 microscope. That was the period when manufacturers were experimenting with a new look for microscope parts, and exactly the same black series appeared for instance in the Leitz / Leica HM-LUX 3 microscope or in the Will Wetzlar V300 / V355 / V365 microscopes.

This objective fits practically any microscope of any make, provided it has an RMS thread and supports objectives with TL = 160 mm and parfocal lengths of 33 / 37 and 45 mm. A few such microscopes are listed in the "compatibility" section, but that list is not complete.

Description of this part for the microscope.

Carl Zeiss Jena
Engraving on the objective: Planachromat 25x/0,50 ∞/0
Magnification: 25x
Numerical aperture N.A.: 0,50
Free working distance: 3,1 mm
Tube length: infinity (more precisely: 250 mm)
Optical correction: planachromatic
Cover glass: no - this objective is intended for work without a cover glass
Immersion: no - this is a so-called dry objective
Phase contrast: no - this objective has no phase ring inside
Polarisation: no - this is not a polarising objective
Thread: M19
Plan compensating eyepieces: yes, required - this objective calls for eyepieces with additional correction (designated "PK" by Carl Zeiss Jena)
Direct projection and macro photography: no - this objective does not work in direct projection mode and is not suitable for macro photography by connecting the objective directly to a camera

This objective can be used as:
- an objective for work in reflected light (EPI), for biological as well as, among others, metallurgical or mineralogical applications
- an objective for work in transmitted light, among others with biological specimens or, for example, with mineralogical thin sections

A few facts about Carl Zeiss Jena objectives with M19 thread (CZJ M19) and their compatibility with various microscopes of this company:
- these objectives serve mostly technical applications and are intended for work without a cover glass (marking ∞/0)
- CZJ M19 objectives intended for biological applications (that is, marked ∞/0,17) are most often used on Carl Zeiss Jena NU / NU2 microscopes
- in Carl Zeiss Jena Nf and Lumipan microscopes, CZJ M19 objectives are used together with the EPI illuminator and can then work both in bright field and in dark field
- in Carl Zeiss Jena Polmi-A microscopes, CZJ M19 objectives can be used, but they give a magnification other than the nominal one
- Carl Zeiss Jena Amplival / Amplival Pol / Amplival Interphako / Peraval microscopes have an interchangeable nosepiece, and there is also a nosepiece intended for these microscopes that accepts objectives with M19 thread
- Carl Zeiss Jena Metaval microscopes use newer versions of CZJ M19 objectives (those with a coarsely knurled rim and a glossy barrel), yet many people experiment with older M19 objectives on these microscopes
- Carl Zeiss Jena Epignost and BK 70x50 microscopes were produced in various configurations - and some work with CZJ M19 objectives
- Carl Zeiss Jena Vertival / Epival / Nf / BK 70x50 microscopes often allow work with CZJ M19 objectives in EPI dark field mode
- on inverted metallographic Carl Zeiss Jena Epityp-2 / Neophot-21 microscopes, many people use biological objectives intended for work with a cover glass alongside metallurgical ones, and place the slide with the specimen on the stage "upside down", that is, with the cover glass facing downwards

Description of this part for the microscope.

objective:
Manufacturer: Zeiss West Germany
Engraving on the objective: Planapo 63/1,4 Oel 160/-
Manufacturer catalogue number: 46 18 40 - 9901 (also written as 461840-9901)
Magnification: 63x
Numerical aperture (N.A.): 1.4
Optical correction: plan-apochromatic
Immersion: yes, oil
Coverslip: according to the engraving on the barrel of this objective, it can be used both without a coverslip and with a standard coverslip of 0.17 mm thickness
Tube length: 160 mm
Parfocal distance: 45 mm
Thread: RMS
Specimen protection: yes, the objective has a spring-loaded mechanism preventing the slide with the specimen from being broken
Phase contrast: no - the objective has no phase rings inside
DIC: prisms allowing work in DIC mode are available on the market for this objective (sold separately)

This objective fits practically any microscope of any manufacturer, provided it has an RMS thread and accepts objectives for a 160 mm tube length with a parfocal distance of 33, 37 or 45 mm. A few such microscopes are listed as examples in the "compatibility" section, but this is not a complete list.

Description of this part for the microscope.

21 microscope.

Basic parameters:
Manufacturer: Carl Zeiss Jena
Engraving on the objective: Planachromat 25x/0,50 ∞/0 Ph
Magnification: 25x
Numerical aperture N.A.: 0,50
Tube length: infinity (more precisely: 250 mm)
Optical correction: planachromatic
Cover glass: no - this objective is intended for work without a cover glass
Immersion: no - this is a so-called dry objective
Phase contrast: yes, a special phase contrast for work in reflected light
Polarisation: no - this is not a polarising objective
Thread: M19
Plan compensating eyepieces: yes, required - this objective needs eyepieces with additional correction (marked "PK" at Carl Zeiss Jena)
Direct projection and macro photography: no - this objective does not work in direct projection mode and is not suitable for macro photography with the objective attached directly to a camera

Phase contrast objectives for the Neophot microscopes have a phase ring built differently from the other Ph objectives made by Carl Zeiss Jena - instead of a continuous ring there is a three-segment pattern, somewhat reminiscent of a Wankel engine rotor. This is worth knowing, because it means that a phase contrast objective from a biological microscope will not replace this one in a Neophot, and the other way round.

The reason is a matter of design. In a metallurgical microscope the objective also acts as the condenser, so the illuminating light passes through the same pupil as the light forming the image. With a continuous ring the whole illumination would pass through the phase coating, which lets only part of the light through and reflects a fair amount of it - and those reflections would swamp the already faint phase contrast image. The solution turned out to be an odd number of segments: the image of the diaphragm formed after reflection off the specimen is rotated by 180 degrees, so with three segments it falls into the gaps between them. The illuminating light therefore enters beside the phase coating and returns through it. The solution is described in patent AT 295883 B.

The objective is offered together with its matching slider (Objektivwechselschlitten) with an M19 thread - and that is no coincidence. The position in which the phase sectors come to rest is decided by how far the objective screws into the thread, and every specimen stops slightly differently, which is why the factory selected objective and slider in pairs and wrote both numbers on the card in the case. Buying such a pair together avoids the whole problem - the objective already sits in relation to its slider exactly as it left the works.

Setting it up in the microscope needs neither a service technician nor a phase telescope - the Neophot has a built-in, swing-out Bertrand lens. The procedure is basically this: switch in the Bertrand lens, focus on the phase sectors and use the adjusting screws to bring the image of the sector diaphragm into coincidence with the image of the sectors in the objective; sometimes a slight rotation of the aperture diaphragm helps as well. Should the position of the glass in the sector diaphragm itself ever need correcting - it is held down by an ordinary threaded ring, so it can be turned. The whole thing resembles centring the phase rings in the condenser of a biological microscope.

Two more things are needed on the microscope side for work in phase contrast: the sector diaphragm (Sektorenblende), pushed in front of the open aperture diaphragm, and the auxiliary lens turret (Hilfslinsenrevolver) on the dovetail of the illuminator. The latter images the diaphragm exactly in the plane of the phase coating, which lies slightly differently in each objective - hence a separate auxiliary lens for every magnification. In bright field the objective of course works perfectly normally.

A note for the DIY-minded: an objective like this can be adapted for transmitted light, except that the diaphragm in the condenser is then set not opposite but in line with the segments of the objective, because there is no second pass through the pupil. Phase contrast should appear, but with stronger halo and a directional relief effect, since the gaps in the ring mean that part of the illumination azimuths is missing. On top of that comes the infinity design with no cover glass correction, so a tube lens will be needed. More of an experiment than a working solution - but doable in one evening.

The last photos in the gallery show:

* a photo of the original table indicating which serial numbers of the objective sliders are factory-paired with which serial numbers of the objectives
* two pages of errata to the instruction manual of the Carl Zeiss Jena Neophot 2 microscope.

The "compatibility" section lists, besides the Carl Zeiss Jena Neophot 21 microscope, those microscopes that this objective fits mechanically and optically. What result you get on them, however, can sometimes be a surprise.

Here (https://www.microscopeparts.eu/part/91056) is the same objective in the version without phase rings. Its description tells you more about using Carl Zeiss Jena objectives with an M19 thread.

Description of this part for the microscope.

21 microscope.

Basic parameters:
Manufacturer: Carl Zeiss Jena
Engraving on the objective: Planachromat 12.5x/0.25 ∞/0 Ph
Magnification: 12.5x
Numerical aperture N.A.: 0.25
Tube length: infinity (more precisely: 250 mm)
Optical correction: planachromatic
Cover glass: no - this objective is intended for work without a cover glass
Immersion: no - this is a so-called dry objective
Phase contrast: yes, a special phase contrast for work in reflected light
Polarisation: no - this is not a polarising objective
Thread: M19
Plan compensating eyepieces: yes, required - this objective needs eyepieces with additional correction (marked "PK" at Carl Zeiss Jena)
Direct projection and macro photography: no - this objective does not work in direct projection mode and is not suitable for macro photography with the objective attached directly to a camera

Phase contrast objectives for the Neophot microscopes have a phase ring built differently from the other Ph objectives made by Carl Zeiss Jena - instead of a continuous ring there is a three-segment pattern, somewhat reminiscent of a Wankel engine rotor. This is worth knowing, because it means that a phase contrast objective from a biological microscope will not replace this one in a Neophot, and the other way round.

The reason is a matter of design. In a metallurgical microscope the objective also acts as the condenser, so the illuminating light passes through the same pupil as the light forming the image. With a continuous ring the whole illumination would pass through the phase coating, which lets only part of the light through and reflects a fair amount of it - and those reflections would swamp the already faint phase contrast image. The solution turned out to be an odd number of segments: the image of the diaphragm formed after reflection off the specimen is rotated by 180 degrees, so with three segments it falls into the gaps between them. The illuminating light therefore enters beside the phase coating and returns through it. The solution is described in patent AT 295883 B.

The objective is offered together with its matching slider (Objektivwechselschlitten) with an M19 thread - and that is no coincidence. The position in which the phase sectors come to rest is decided by how far the objective screws into the thread, and every specimen stops slightly differently, which is why the factory selected objective and slider in pairs and wrote both numbers on the card in the case. Buying such a pair together avoids the whole problem - the objective already sits in relation to its slider exactly as it left the works.

Setting it up in the microscope needs neither a service technician nor a phase telescope - the Neophot has a built-in, swing-out Bertrand lens. The procedure is basically this: switch in the Bertrand lens, focus on the phase sectors and use the adjusting screws to bring the image of the sector diaphragm into coincidence with the image of the sectors in the objective; sometimes a slight rotation of the aperture diaphragm helps as well. Should the position of the glass in the sector diaphragm itself ever need correcting - it is held down by an ordinary threaded ring, so it can be turned. The whole thing resembles centring the phase rings in the condenser of a biological microscope.

Two more things are needed on the microscope side for work in phase contrast: the sector diaphragm (Sektorenblende), pushed in front of the open aperture diaphragm, and the auxiliary lens turret (Hilfslinsenrevolver) on the dovetail of the illuminator. The latter images the diaphragm exactly in the plane of the phase coating, which lies slightly differently in each objective - hence a separate auxiliary lens for every magnification. In bright field the objective of course works perfectly normally.

A note for the DIY-minded: an objective like this can be adapted for transmitted light, except that the diaphragm in the condenser is then set not opposite but in line with the segments of the objective, because there is no second pass through the pupil. Phase contrast should appear, but with stronger halo and a directional relief effect, since the gaps in the ring mean that part of the illumination azimuths is missing. On top of that comes the infinity design with no cover glass correction, so a tube lens will be needed. More of an experiment than a working solution - but doable in one evening.

The last photos in the gallery show:

* a photo of the original table indicating which serial numbers of the objective sliders are factory-paired with which serial numbers of the objectives
* two pages of errata to the instruction manual of the Carl Zeiss Jena Neophot 2 microscope.

The "compatibility" section lists, besides the Carl Zeiss Jena Neophot 21 microscope, those microscopes that this objective fits mechanically and optically. What result you get on them, however, can sometimes be a surprise.

Here (https://www.microscopeparts.eu/part/91056) you can read more about using Carl Zeiss Jena objectives with an M19 thread.

Description of this part for the microscope.

21 microscope.

Basic parameters:
Manufacturer: Carl Zeiss Jena
Engraving on the objective: Planachromat 6.3x/0.12 ∞/0 Ph
Magnification: 6.3x
Numerical aperture N.A.: 0.12
Tube length: infinity (more precisely: 250 mm)
Optical correction: planachromatic
Cover glass: no - this objective is intended for work without a cover glass
Immersion: no - this is a so-called dry objective
Phase contrast: yes, a special phase contrast for work in reflected light
Polarisation: no - this is not a polarising objective
Thread: M19
Plan compensating eyepieces: yes, required - this objective needs eyepieces with additional correction (marked "PK" at Carl Zeiss Jena)
Direct projection and macro photography: no - this objective does not work in direct projection mode and is not suitable for macro photography with the objective attached directly to a camera

Phase contrast objectives for the Neophot microscopes have a phase ring built differently from the other Ph objectives made by Carl Zeiss Jena - instead of a continuous ring there is a three-segment pattern, somewhat reminiscent of a Wankel engine rotor. This is worth knowing, because it means that a phase contrast objective from a biological microscope will not replace this one in a Neophot, and the other way round.

The reason is a matter of design. In a metallurgical microscope the objective also acts as the condenser, so the illuminating light passes through the same pupil as the light forming the image. With a continuous ring the whole illumination would pass through the phase coating, which lets only part of the light through and reflects a fair amount of it - and those reflections would swamp the already faint phase contrast image. The solution turned out to be an odd number of segments: the image of the diaphragm formed after reflection off the specimen is rotated by 180 degrees, so with three segments it falls into the gaps between them. The illuminating light therefore enters beside the phase coating and returns through it. The solution is described in patent AT 295883 B.

The objective is offered together with its matching slider (Objektivwechselschlitten) with an M19 thread - and that is no coincidence. The position in which the phase sectors come to rest is decided by how far the objective screws into the thread, and every specimen stops slightly differently, which is why the factory selected objective and slider in pairs and wrote both numbers on the card in the case. Buying such a pair together avoids the whole problem - the objective already sits in relation to its slider exactly as it left the works.

Setting it up in the microscope needs neither a service technician nor a phase telescope - the Neophot has a built-in, swing-out Bertrand lens. The procedure is basically this: switch in the Bertrand lens, focus on the phase sectors and use the adjusting screws to bring the image of the sector diaphragm into coincidence with the image of the sectors in the objective; sometimes a slight rotation of the aperture diaphragm helps as well. Should the position of the glass in the sector diaphragm itself ever need correcting - it is held down by an ordinary threaded ring, so it can be turned. The whole thing resembles centring the phase rings in the condenser of a biological microscope.

Two more things are needed on the microscope side for work in phase contrast: the sector diaphragm (Sektorenblende), pushed in front of the open aperture diaphragm, and the auxiliary lens turret (Hilfslinsenrevolver) on the dovetail of the illuminator. The latter images the diaphragm exactly in the plane of the phase coating, which lies slightly differently in each objective - hence a separate auxiliary lens for every magnification. In bright field the objective of course works perfectly normally.

A note for the DIY-minded: an objective like this can be adapted for transmitted light, except that the diaphragm in the condenser is then set not opposite but in line with the segments of the objective, because there is no second pass through the pupil. Phase contrast should appear, but with stronger halo and a directional relief effect, since the gaps in the ring mean that part of the illumination azimuths is missing. On top of that comes the infinity design with no cover glass correction, so a tube lens will be needed. More of an experiment than a working solution - but doable in one evening.

The last photos in the gallery show:

* a photo of the original table indicating which serial numbers of the objective sliders are factory-paired with which serial numbers of the objectives
* two pages of errata to the instruction manual of the Carl Zeiss Jena Neophot 2 microscope.

The "compatibility" section lists, besides the Carl Zeiss Jena Neophot 21 microscope, those microscopes that this objective fits mechanically and optically. What result you get on them, however, can sometimes be a surprise.

Here (https://www.microscopeparts.eu/part/91056) you can read more about using Carl Zeiss Jena objectives with an M19 thread.